The interaction of thrombomodulin with Ca2+

European Journal of Biochemistry

Volumn 262, Issue 2, 1999, Pages 522-533

  Light, David R ^a   Glaser, Charles B ^a   Betts, Melissa ^a   Blasko, Eric ^a   Campbell, Elizabeth ^a   Clarke, Jeffrey H ^a   McCaman, Michael ^a   McLean, Kirk ^a   Nagashima, Mariko ^a   Parkinson, John F ^a   Rumennik, Galina ^a   Young, Tish ^a   Morser, John ^a  

^a BAYER AG   (Germany)

Author keywords

Calcium; Epidermal growth factor domain; Protein C; Thrombin; Thrombomodulin

Indexed keywords

CALCIUM ION; PROTEIN C; THROMBIN; THROMBOMODULIN; CALCIUM; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN; TRYPSIN;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CALCIUM BINDING; CONFORMATIONAL TRANSITION; ENZYME ACTIVATION; EPITOPE MAPPING; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; ANIMAL; ARMYWORM; BINDING SITE; CHEMISTRY; CHO CELL; HAMSTER; HUMAN; HYDROLYSIS; METABOLISM;

ANIMALS; ANTIBODIES, MONOCLONAL; BINDING SITES; CALCIUM; CHO CELLS; CRICETINAE; HUMANS; HYDROLYSIS; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPODOPTERA; THROMBOMODULIN; TRYPSIN;

EID: 0033146801     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00398.x     Document Type: Article

References (40)

1. 1. Sadler, J.E. (1997) Thrombomodulin structure and function. Thromb. Haemost. 78, 392-395.
2. 2. Kurosawa, S., Galvin, J.B., Esmon, N.L. & Esmon, C.T. (1987) Proteolytic formation and properties of functional domains of thrombomodulin. J. Biol. Chem. 262, 2206-2212.
3. 3. Kurosawa, S., Stearns, D.J., Jackson, K.W. & Esmon, C.T. (1988) A 10-kDa cyanogen bromide fragment from the epidermal growth factor homology domain of rabbit thrombomodulin contains the primary thrombin binding site. J. Biol. Chem. 263, 5993-5996.
4. 4. Zushi, M., Gomi, K., Yamamoto, S., Maruyama, I., Hayashi, T. & Suzuki, K. (1989) The last three consecutive epidermal growth factor-like structures of human thrombomodulin comprise the minimum functional domain for protein C-activating cofactor activity and anticoagulant activity. J. Biol. Chem. 264, 10351-10353.
5. 5. Hayashi, T., Zushi, M., Yamamoto, S. & Suzuki, K. (1989) Further localization of binding sites for thrombin and protein C in human thrombomodulin. J. Biol. Chem. 265, 20156-20159.
6. 6. Parkinson, J.F., Nagashima, M., Kuhn, I., Leonard, J. & Morser, J. (1992) Structure-function studies of the epidermal growth factor domains of human thrombomodulin. Biochem. Biophys. Res. Commun. 185, 567-576.
7. 7. Clarke, J.H., Light, D.R., Blasko, E., Parkinson, J.F., Nagashima, M., McLean, K., Vilander, L., Andrews, W.H., Morser, J. & Glaser, C.B. (1993) The short loop between EGF-like domains four and five is critical for human thrombomodulin function. J. Biol. Chem. 268, 6309-6315.
8. 8. Handford, P.A., Baron, M., Mayhew, M., Willis. A., Beesley, T., Brownlee, G.G. & Campbell, I.D. (1990) The first EGF-like domain from human factor IX contains a high-affinity calcium binding site. EMBO J. 9, 475-480.
9. 9. Davis, C.G. (1990) The many faces of epidermal growth factor repeats. New Biologist 2, 410-419.
10. 10. Stenflo, J., Öhlin, A.-K., Owen, W.G. & Schneider, W.J. (1988) β-Hydroxyaspartic acid or β-hydroxyasparagine in bovine low density lipoprotein receptor and in bovine thrombomodulin. J. Biol. Chem. 263, 21-24.
11. 2+ binding and biological activity. J. Biol. Chem. 263, 19240-19248.
12. 12. Dahlback, B., Bergisa, H. & Linse, S. (1990) Novel type of very high affinity calcium-binding sites in β-hydroxy-asparagine-containing epidermal growth factor-like domains in vitamin K-dependent protein S. J. Biol Chem. 265, 18481-18489.
13. 13. Rees, D.J.G., Jones, I.M., Handford, P.A., Walter, S.J., Esnouf, M.P., Smith, K.J. & Brownlee, G.G. (1988) The role of β-hydroxyaspartate and adjacent carboxylate residues in the first EGF domain of human factor IX. EMBO J. 7, 2053-2061.
14. 14. Sunnerhagen, M.S., Persson, E., Dahlqvist, I., Drakenberg, T., Stenflo, J., Mayhew, M., Robin, M., Handford, P., Tilley, J.W., Campbell, I.D. & Brownlee, G.G. (1993) The effect of aspartate hydroxylation on calcium binding to epidermal growth factor-like modules in coagulation factors IX and X. J. Biol. Chem. 268, 23339-23344.
15. 15. Glaser, C.B., Morser, J., Clarke, J.H., Blasko, E., McLean, K., Kuhn, I., Chang, R.-J., Lin, J.-H., Vilander, V., Andrews, W., H. & Light, D.R. (1992). Oxidation of a specific methionine in thrombomodulin by activated neutrophil products blocks cofactor activity - potential rapid mechanism for modulation of coagulation. J. Clin. Invest. 90, 2565-2573.
16. 16. Weisel, J.W., Nagaswami, C., Young, T.A. & Light, D.R. (1996) The shape of thrombomodulin and interactions with thrombin as determined by electron microscopy. J. Biol. Chem. 271, 31485-31490.
17. 17. Nagashima, M., Lundh, E., Leonard, J.C., Morser, J. & Parkinson, J.F. (1993) Alanine-scanning mutagenesis of the epidermal growth factor-like domains of human thrombomodulin identified critical residues for its cofactor activity. J. Biol. Chem. 268, 2888-2892.
18. 18. Chang, C.T., Wu, C.-S.C. & Yang, J.T. (1978) Circular dichroic analysis of protein conformation: inclusion of the beta-turns. Anal. Biochem. 91, 13-31.
19. 19. Lin, J.-H., McLean, K., Morser, J., Young, T.A., Wydro, R.M., Andrews, W.H. & Light, D.R. (1994) Modulation of glycosaminoglycan addition in naturally expressed and recombinant human thrombomodulin. J. Biol. Chem. 269, 25021-25030.
20. 20. Kumazaki, T., Fujitani, A., Terasawa, K., Shimura, K., Kasai, K. & Ishii, S. (1988) Immobilized anhydrochymotrypsin as a biospecific affinity adsorbent for the peptides produced by chymotryptic hydrolysis. J. Biochem. 103, 297-301.
21. 21. Parkinson. J.F., Grinnell, B.W., Moore, R., Hoskins, J., Vlahos, C.J. & Bang, N.U. (1990) Stable expression of a secretable deletion mutant of recombinant human thrombomodulin in mammalian cells. J. Biol. Chem. 265, 12602-12610.
22. 22. Nelson, R.M. & Long, G.L. (1991) Solution-phase equilibrium binding interaction of human protein S with C4b-binding protein. Biochemistry 30, 2384-2390.
23. 23. Sasaki, T., Göhring, W., Pan, T.-C., Chu, M.-L. & Timpl, T. (1995) Binding of mouse and human fibulin-2 to extracellular matrix ligands. J. Mol. Biol. 254, 892-899.
24. 24. Harris, R.J. & Spellman, M.W. (1993) O-linked fucose and other post-translational modifications unique to EGF modules. Glycobiology 3. 219-224.
25. 25. Wang, Q.P., VanDusen, W.J., Petroski, C.J., Garsky, V.M., Stern, A.M. & Friedman, P.A. (1991) Bovine liver aspartyl β-hydroxylase. Purification and characterization. J. Biol. Chem. 266, 14004-14010.
26. 26. Kimura, S., Nagoya, T. & Aoki, N. (1989) Monoclonal antibodies to human thrombomodulin whose binding is calcium dependent. J. Biochem. 105, 478-483.
27. 27. Tolkatchev, D. & Ni, F. (1998) Calcium binding properties of an epidermal growth factor-like domain from human thrombomodulin. Biochemistry 37, 9091-9100.
28. 28. Adler, M., Seto, M.H., Nitecki, D.E., Lin, J.-H., Light, D.R. & Morser, J. (1995) The structure of a 19-residue fragment from the C-loop of the fourth epidermal growth factor-like domain of thrombomodulin. J. Biol. Chem. 270, 23366-23372.
29. 29. Hunter, M.J. & Komives, E.A. (1995) Thrombin-binding affinities of different disulfide-bonded isomers of the fifth EGF-like domain of thrombomodulin. Protein Sci. 4, 2129-2137.
30. 30. White, C.E., Hunter, M.J., Meininger, D.P., Garrod, S. & Komives, E. (1996) The fifth epidermal growth factor-like domain of thrombomodulin does not have an epidermal growth factor-like disulfide bonding pattern. Proc. Natl Acad. Sci. USA 93, 10177-10182.
31. 31. Dittman, W.A., Kumada, T., Sadler, J.E. & Majerus, P.W. (1988) The structure and function of mouse thrombomodulin. Phorbol myristate acetate stimulates degradation and synthesis of thrombomodulin without affecting mRNA levels in hemangioma cells. J. Biol. Chem. 263, 15815-15822.
32. 32. Kunihiro, Y., Tanaka, R., Ichimura, M., Uemura, A., Ohzawa, N. & Mochida, E. (1995) Purified glycoprotein having a high affinity for thrombin stimulates thrombin-catalyzed protein C activation - useful as an anticoagulant for treating and preventing disseminated intravascular coagulation, thrombosis peripheral blood vessel obstruction etc. European Patent EP-632055-AI.
33. 33. Esmon, N.L., Owen, W.G. & Esmon, C.T. (1982) Isolation of a membrane-bound cofactor for thrombin-catalyzed activation of protein C. J. Biol. Chem. 257, 859-864.
34. 34. Esmon, N.L., Carroll, R.C. & Esmon, C.T. (1983) Thrombomodulin blocks the ability of thrombin to activate platelets. J. Biol. Chem. 258, 12238-12242.
35. 2+ binding to a high affinity site that does not contain γ-carboxyglutamic acid. J. Biol. Chem. 258, 5554-5560.
36. 2+ for rapid activation by the thrombin-thrombomodulin complex. J. Biol. Chem. 269, 3151-3154.
37. 2+-mediated binding to protein C. J. Biol. Chem. 266, 19886-19889.
38. 38. Sugo, T., Björk, I., Holmgren, A. & Stenflo, J. (1984) Calcium-binding properties of bovine factor X lacking the γ-carboxylate acid-containing region. J. Biol. Chem. 259, 5705-5710.
39. 2+ binding site yet retains function. J. Biol. Chem. 269, 21495-21499.
40. 40. Villoutreix, B.O. & Dahlbäck, B. (1998) Molecular model for the C-type lectin domain of human thrombomodulin. J. Mol. Model. 4, 310-322.