Cloning and expression in escherichia coli of a gene coding for a secondary alcohol dehydrogenase from candid

Bioscience, Biotechnology and Biochemistry

Volumn 63, Issue 6, 1999, Pages 1051-1055

  Yamamoto, Hiroaki ^a   Kawada, Naoki ^a   Matsuyama, Akinobu ^a   Kobayashi, Yoshinori ^a  

^a DAICEL CORPORATION   (Japan)

Author keywords

(R) 1,3 butanediol; (S) 1,3 butanediol dehydrogenase; Candida parapsilosis; NAD+ regeneration; Secondary alcohol dehydrogenase

Indexed keywords

CANDIDA PARAPSILOSIS; ESCHERICHIA COLI;

ALCOHOL DEHYDROGENASE; FUNGAL DNA; PRIMER DNA; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CANDIDA; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ALCOHOL DEHYDROGENASE; AMINO ACID SEQUENCE; BASE SEQUENCE; CANDIDA; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DNA PRIMERS; DNA, FUNGAL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, ENZYMOLOGIC; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 0033144136     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.63.1051     Document Type: Article

References (12)

1. Nakatsuka, T., Iwata, H., Tanaka, R., Imajo, S., and Ishiguro, M., A facile conversion of the phenylthio group to acetoxy by copper reagents for a practical synthesis of 4-acetoxyazetidin-2-one derivatives from (i?)-butane-l,3-diol. J. Chern. Soc., Chem. Commun., 662-664 (1991).
2. Matsuyama, A., Kobayashi, Y., and Ohnishi, H., Microbial production of optically active 1,3-butanediol from the racemate. Biosci. Biotechnol. Bio chem., 57, 685-585 (1993).
3. Yamamoto, H., Matsuyama, A., Kobayashi, Y., and Kawada, N., Purification and characterization of (S)-l,3-butanediol dehydrogenase from Candida parapsilosis. Biosci. Biotechnol. Biochem., 59, 1769-1770 (1995).
4. Maniatis, T., Fritsch, E. F., and Sambrook, J., “Molecular Cloning: A Laboratory Manual,” 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, (1989).
5. Cryer, D. R., Eccleshall, R., and Marmur, J., Isolation of yeast DNA. Meth. Cell Biol., 33, 39-44 (1975).
6. Ochman, H., Gerber, A. S., and Hartl, D. L., Genetic applications of an inverse polymerase chain reaction. Genetics, 120, 621-623 (1988).
7. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).
8. Ohama, T., Suzuki, T., Mori, M., Osawa, S., Ueda, T., Watanabe, K., and Nakase, T., Non-universal decoding of the leucine codon CUG in several Candida species. Nucleic Acids Res., 21, 4039-4045 (1993).
9. Reid, M. F. And Fewson, C. A., Molecular characterization of microbial alcohol dehydrogenases. Crit. Rev. Microbiol., 20, 13-56 (1994).
10. Pearson, W. R. And Lipman, D. J., Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. U.S.A., 85, 2444-2448 (1988).
11. Lutstorf, U. And Megnet, R., Multiple forms of alcohol dehydrogenases in Saccharomyces cerevisiae. Arch. Biochem. Biophys., 126, 933-944 (1968).
12. Kataoka, M., Rohani, L. P. S., Yamamoto, K., Wada, M., Kawabata, H., Kita, K., Yanase, H., and Shimizu, S., Enzymatic production of ethyl (J?)-4-chloro-3-hydroxybutanoate: asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by an Escherichia coli transformant expressing the aldehyde reductase gene from yeast. Appl. Microbiol. Biotechnol., 48, 699-703 (1997).