Cloning and expression of ecto 5'-nucleotidase from the cattle tick Boophilus microplus

Insect Molecular Biology

Volumn 8, Issue 2, 1999, Pages 257-266

  Liyou, N ^a   Hamilton, S ^b   Elvin, C ^a   Willadsen, P ^a  

^a CSIRO   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

5' nucleotidase; Boophilus microplus

Indexed keywords

CDNA LIBRARY; CLONING; ECTO 5' NUCLEOTIDASE; ENZYME ACTIVITY; GENE EXPRESSION; HYDROLYSIS; INTRON; MAMMALIAN GENETICS; SOUTHERN BLOTTING;

ACARI; ANIMALIA; ARTHROPODA; BOOPHILUS MICROPLUS; BOS TAURUS; ESCHERICHIA COLI; FELIS CATUS; INSECTA; MAMMALIA; PROTOZOA; UNIDENTIFIED BACULOVIRUS; VERTEBRATA;

EID: 0033136411     PISSN: 09621075     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2583.1999.820257.x     Document Type: Article

References (26)

1. Becker, J.L. (1978) Regulation of purine biosynthesis in cultured Drosophila melanogaster cells: I. Conditional activity of hypoxanthine-guanine-phosphoribosyltransferase and 5-nucleotidase. Biochimie 60: 619-625.
2. Burns, D.M. and Beacham, I.R. (1986) Nucleotide sequence and transcriptional analysis of the E coli ushA gene, encoding periplasmic UDP-sugar hydrolase (5′-nucleotidase): regulation of the ushA gene, and the signal sequence of its encoded protein product. Nucl Acids Res 14: 4325-4341.
3. Champagne, D.E., Smartt, C.T., Ribeiro, J.M.C. and James, A.A. (1995) The salivary gland-specific apyrase of the mosquito Aedes aegypti is a member of the 5′-nucleotidase family. Proc Natl Acad Sci USA 92: 694-698.
4. Chou, Q., Russell, M., Birch, D.E., Raymond, J. and Bloch, W. (1992) Prevention of pre-PCR mis-priming dimerization improves low-copy-number amplification. Nucl Acids Res 20: 1717-1723.
5. Dvorak, H.F. and Heppel, L.A. (1968) Métallo-enzymes released from Escherichia coli by osmotic shock. J Biol Chem 213: 2647-2653.
6. Fini, C., Palmerini, C.A., Damiani, P., Stochaj, U., Mannherz, H.G. and Floridi, A. (1990) 5′-Nucleotidase from bull seminal plasma, chicken gizzard and snake venom is a zinc metalloprotein. Biochim Biophys Acta 1038:18-22.
7. Glaser, L., Melo, A. and Paul, R. (1966) Uridine diphosphate sugar hydrolase: purification of enzyme and protein inhibitor. J Biol Chem 242: 1944-1954.
8. Groppe, J.C. and Morse, D.E. (1993) Isolation of full-length RNA templates for reverse transcription from tissues rich in RNase and proteoglycans. Anal Biochem 210: 337-343.
9. Henry, J.M., Raina, A.K. and Ridgway, R.L. (1990) Isolation of high molecular weight DNA from insects. Anal Biochem 185: 147-150.
10. Itami, H., Sakai, Y., Shimamoto, T., Hama, H., Tsuda, M. and Tsuchiya, T. (1989) Purification and characterisation of membrane-bound 5′-nucleotidase of Vibrio parahaemolyticus. J Biochem 105: 785-789.
11. Klabunde, T., Strater, N., Frolich, R., Witzel, H. and Krebs, B (1996) Mechanism of Fe(III)-Zn(II) purple acid phosphatase based on crystal structures. J Mol Biol 259: 737-748.
12. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature 227: 680-685.
13. Murray, A.W. (1971) The biological significance of purine salvage. Annu Rev Biochem 40: 811-826.
14. Neu, H.C. (1967) The 5′-nucleotidase of Escherichia coli. J Biol Chem 242: 3896-3904.
15. Rabilloud, T., Carpentier, G. and Tarroux, P. (1988) Improvement and simplification of low-background silver staining of proteins by using sodium dithionite. Electrophoresis 9: 288-291.
16. Sambrook, J., Fritsch, E.F. and Maniatis, T., eds. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Schneppenheim, R. and Rautenberg, P. (1987) A luminescence western blot with enhanced sensitivity for antibodies to human immunodeficiency virus. Eur J Clin Microbiol 6: 49-51.
18. Stanley, K.K., Newby, A.C. and Luzio, J.P. (1982) What do ectoenzymes do? TIBS T. 145-147.
19. Stone, T.W. (1981) Physiological roles for adenosine and adenosine 5′-triphosphate in the nervous system. Neuroscience 6:523-555.
20. Stuber, D., Matile, H. and Garotta, G. (1990) System for high-level production in E coli and rapid purification of recombinant proteins: application to epitope mapping, preparation of antibodies, and structure-function analysis, Immunological Methods (Lefkovits, I. and Pernis, B. eds.), pp. 121-152. Academic Press, New York.
21. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
22. West, S., Schroder, J. & Kunz, W. (1990) A multiple-staining procedure for the detection of different DNA fragments on a single blot. Anal Biochem 190: 254-258.
23. Willadsen, P. and McKenna, R.V. (1991) Vaccination with 'concealed' antigens: myth or reality? Parasite Immunol 13: 605-616.
24. Willadsen, P., Nielsen, J.M. and Riding, G.A. (1989) Purification and properties of a novel nucleotide-hydrolysing enzyme (5′-nucleotidase) from Boophilus microplus. Biochem J 258: 79-85.
25. Willadsen, P., Riding, G.A., Jarmey, J. and Atkins, A. (1993) The nucleotidase of Boophilus microplus and its relationship to enzymes from the rat and Escherichia coli. Insect Biochem Mol Biol 23: 291-295.
26. Zimmermann, H. (1992) 5′-nucleotidase: molecular structure and functional aspects. Biochem J 285: 345-365.