Human high-K(m) 5'-nucleotidase. Effects of overexpression of the cloned cDNA in cultured human cells

European Journal of Biochemistry

Volumn 261, Issue 3, 1999, Pages 689-697

  Rampazzo, Chiara ^c   Gazziola, Cinzia ^c   Ferraro, Paola ^c   Gallinaro, Lisa ^c   Johansson, Magnus ^a   Reichard, Peter ^c   Bianchi, Vera ^b  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b UNIVERSITY OF PADOVA   (Italy)

^c NONE

Author keywords

GFP protein; Nucleotidase; Nucleotide pools; Substrate cycle

Indexed keywords

5' NUCLEOTIDASE; COMPLEMENTARY DNA; DEOXYADENOSINE; DEOXYGUANOSINE; DEOXYRIBONUCLEOTIDE; DIDANOSINE; HYBRID PROTEIN; MESSENGER RNA; GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN; PRIMER DNA; RECOMBINANT PROTEIN; RIBONUCLEOSIDE;

ALLOSTERISM; ARTICLE; CELL INCLUSION; CELL MEMBRANE; CITRIC ACID CYCLE; CONTROLLED STUDY; CYTOSOL; ENZYME ACTIVITY; ENZYME KINETICS; ESCHERICHIA COLI; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; MICHAELIS CONSTANT; MOLECULAR CLONING; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; CELL CULTURE; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; WESTERN BLOTTING;

5'-NUCLEOTIDASE; BASE SEQUENCE; BLOTTING, WESTERN; CELLS, CULTURED; CLONING, MOLECULAR; DIDANOSINE; DNA PRIMERS; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HUMANS; JURKAT CELLS; LUMINESCENT PROTEINS; PHOSPHORYLATION; RECOMBINANT PROTEINS; RIBONUCLEOSIDES; TRANSFECTION;

EID: 0033125735     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00320.x     Document Type: Article

References (41)

1. 1. Zimmermann, H. (1992) 5′-Nucleotidase: molecular structure and functional aspects. Biochem. J. 285, 345-365.
2. 2. Itoh, R. (1992) IMP-GMP 5′-nucleotidase. Comp. Biochem. Physiol. 105B, 13-19.
3. 3. Fritzson, P. (1978) Regulation of nucleotidase activities in animal tissues. Adv. Ezyme Regul. 16, 43-61.
4. 4. Arnèr, E.S.J. & Eriksson, S. (1995) Mammalian deoxyribonucleoside kinases. Pharmac. Ther. 67, 155-186.
5. 5. Kauffman, M.G. & Kelly, T.J. (1991) Cell cycle regulation of thymidine kinase: residues near the carboxyl terminus are essential for the specific degradation of the enzyme at mitosis. Mol. Cell. Biol. 11, 2538-2546.
6. 6. Wintersberger, E., Rotheneder, H., Grabner, M., Beck, G. & Seiser, C. (1992) Regulation of thymidine kinase during growth, cell cycle and differentiation. Adv. Enzyme Regul. 32, 241-254.
7. 7. Dou, Q.-P. & Pardee, A.B. (1996) Transcriptional activation of thymidine kinase, a marker for cell cycle control. Progr. Nucleic Acid Res. Mol. Biol. 53, 197-217.
8. 8. Bohman, C. & Eriksson, S. (1988) Deoxycytidine kinase from human leukemic spleen: preparation and characterization of the homogeneous enzyme. Biochemistry 27, 4258-4265.
9. 9. Mitchell, B.S., Song, J.J., Johnson-II, E.E., Chen, E.& Dayton J.S., (1993) Regulation of human deoxycytidine kinase expression. Adv. Enzyme Regul. 33, 61-68.
10. 10. Johansson, M., Brismar, S. & Karlsson, A. (1997) Human deoxycytidine kinase is located in the cell nucleous. Proc. Natl Acad. Sci. USA 94, 11941-11945.
11. 11. Hatzis, P., Al-Madhoon, A.S., Jüllig, M., Petrakis, T.G., Eriksson, S. & Talianidis, I. (1998) The intracellular localization of deoxycytidine kinase. J. Biol. Chem. 273, 30239-30243.
12. 12. Kornberg, A. & Baker, T.A. (1992) DNA Replication, 2nd edn. W.H. Freeman and Co., New York, USA.
13. 13. Bianchi, V., Pontis, E. & Reichard, P. (1986) Interrelations between substrate cycles and de novo synthesis of pyrimidine deoxyribonucleoside triphosphates in 3T6 cells. Proc. Natl Acad. Sci. USA 83, 986-990.
14. 14. Reichard, P. (1988) Interactions between deoxyribonucleotide and DNA synthesis. Annu. Rev. Biochem. 57, 349-374.
15. 15. Bianchi, V., Ferraro, P., Borella, S., Bonvini, P. & Reichard, P. (1994) Effects of mutational loss of nucleoside kinases on deoxyadenosine 5′-phosphate/deoxyadenosine substrate cycle in cultured CEM and V79 cells. J. Biol. Chem. 269, 16677-16683.
16. 16. Griffith, D.A. & Jarvis, S.M. (1996) Nucleoside and nucleobase transport systems of mammalian cells. Biochim. Biophys. Acta 1286, 153-181.
17. 17. Martin Jr, D.W. & Gelfand, E.W. (1981) Biochemistry of diseases of immuno-developement. Annu. Rev. Biochem. 50, 845-877.
18. 18. Page, T., Yu, A., Fontanesi, J. & Nyhan, W.L. (1997) Developmental disorder associated with increased cellular nucleotidasse activity. Proc. Natl Acad. Sci. USA 94, 11601-11606.
19. 19. Höglund, L., Pontis, E. & Reichard, P. (1988) Effects of deoxycytidine and thymidine kinase deficiency on substrate cycles between deoxyribonucleosides and their 5′-phosphates. Cancer Res. 48, 3681-3687.
20. 20. Truong, V.L., Collinson, A.R. & Lowenstein, J.M. (1988) 5′-nucleotidases in rat heart. Evidence for the occurrence of two soluble enzymes with different substrates. Biochem. J. 253, 117-121.
21. 21. Yamazaki, Y., Truong, V. & Lowenstein, J.M. (1991) 5′-nucleotidase I from rabbit heart. Biochemstry 30, 1503-1509.
22. 22. Resta, R., Yamashita, Y. & Thompson, L. (1998) Ecto-enzyme and signaling functions of lymphocyte CD73. Immunol. Rev. 161, 95-109.
23. 23. Madrid-Marina, V. & Fox, I.H. (1986) Human placental cytoplasmic 5′-nucleotidase. J. Biol. Chem. 261, 444-452.
24. m' 5′-nucleotidase of rat kidney represents solubilized ecto-5′-nucleotidase. Biochem. J. 273, 409-413.
25. 25. Resta, R., Hooker, S.W., Laurent, A.B., Shuck, J.K., Misumi, Y., Ikehara, Y., Koretzky, G.A. & Thompson, L.F. (1994) Glycosyl phosphatidylinositol membrane anchor is not required for T cell activation through CD73. J. Immunol. 153, 1046-1053.
26. 26. Gutensohn, W., Resta, R., Misumi, Y., Ikehara, Y. & Thompson, L.F. (1995) Ecto-5′-nucleotidase activity is not required for T cell activation through CD73. Cell. Immunol. 161, 213-217.
27. m soluble 5′-nucleotidase from human placenta. Properties and allosteric regulation by IMP and ATP. J. Biol. Chem. 263, 18759-18765.
28. 28. Johnson, M.A. & Fridland, A. (1989) Phosphorylation of 2′,3′-dideoxyinosine by cytosolic 5′-nucleotidase of human lymphoid cells. Mol. Pharmacol. 36, 291-295.
29. 29. Pesi, R., Turriani, M., Allegrini, S., Scolozzi, C., Camici, M., Ipata, P.L. & Tozzi, M.G. (1994) The bifunctional cytosolic 5′-nucleotidase: regulation of the phosphotransferase and nucleotidase activities. Arch. Biochem. Biophys. 312, 75-80.
30. 30. Fritzson, P. & Smith, I. (1971) A new nucleotidase of rat liver with activity toward 3′- and 5′-nucleotidase. Biochem. Biophys. Acta 235, 128-141.
31. 31. Magnusson, G. (1971) Deoxyribonucleotide phosphatase from rat liver and cultured mouse fibroblasts. Eur. J. Biochem. 20, 225-230.
32. 32. Höglund, L. & Reichard, P. (1990) Cytoplasmic 5′ (3′)-nucleotidase from human placenta. J. Biol. Chem. 265, 6589-6595.
33. 33. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning, a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
34. 34. Oka, J., Matsumoto, A., Hosokawa, Y. & Inoue, S. (1994) Molecular cloning of human cytosolic purine 5′-nucleotidase. Biochem. Biophys. Res. Comm. 205, 917-922.
35. 35. Höglund, L. & Reichard, P. (1990) Nucleotidase activities in soluble and membrane fractions of three different mammalian cell lines. Exp. Cell Res. 190, 204-208.
36. 36. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72, 248-254.
37. 37. Fortunati, E. & Bianchi, V. (1991) Spectrophotometric determination of total macromolecules for the evaluation of cellular density in cytotoxicity tests. ATLA 19, 119-125.
38. 38. Sherman, P.A. & Fyfe, J.A. (1989) Enzymatic assay for deoxyribonucleoside triphophates using synthetic oligonucleotides as template primers. Analyt. Biochem. 180, 222-226.
39. 39. Itoh, R. & Yamada, K. (1991) Determination of cytoplasmic 5′-nucleotidase which preferentially hydrolyses 6-hydroxypurine nucleotides in pig, rat and human tissues by immunotitration. Int. J. Biochem. 23, 461-465.
40. 40. Allegrini, S., Pesi, R., Tozzi, M.G., Fiol, C.J., Johnson, R.B. & Eriksson, S. (1997) Bovine cytosolic IMP/GMP-specific 5′-nucleotidase: cloning and expression of active enzyme in Escherichia coli. Biochem. J. 328, 483-487.
41. 41. Oka, J., Ozasa, H. & Itoh, R. (1988) A reappraisal of the subunit molecular mass of chicken liver cytosol 5′-nucleotidase. Biochim. Biophys. Acta 953, 114-118.