Structure and hormone responsiveness of the gene encoding the α-subunit of the rat amiloride-sensitive epithelial sodium channel

American Journal of Respiratory Cell and Molecular Biology

Volumn 20, Issue 5, 1999, Pages 1028-1040

  Otulakowski, Gail ^a   Rafii, Bijan ^a   Bremner, Harry Robert ^a   O'Brodovich, Hugh ^a  

^a HOSPITAL FOR SICK CHILDREN RESEARCH INSTITUTE   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; EPITHELIAL SODIUM CHANNEL; MESSENGER RNA; SODIUM CHANNEL;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL LINE; CELL STRAIN COS1; EXON; GENETIC TRANSCRIPTION; GENETICS; HUMAN; INTRON; METABOLISM; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PROMOTER REGION; RAT; REPORTER GENE; SEQUENCE HOMOLOGY; WISTAR RAT;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; COS CELLS; DNA, COMPLEMENTARY; EPITHELIAL SODIUM CHANNEL; EXONS; GENES, REPORTER; HUMANS; INTRONS; PROMOTER REGIONS (GENETICS); RATS; RATS, WISTAR; RNA, MESSENGER; SEQUENCE HOMOLOGY, AMINO ACID; SODIUM CHANNELS; TRANSCRIPTION, GENETIC;

EID: 0033124960     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/ajrcmb.20.5.3382     Document Type: Article

References (59)

1. Canessa, C. M., J. D. Horisherger, and B. C. Rossier. 1993. Epithelial sodium channel related to proteins involved in neurodegeneration. Nature 361:467-470.
2. Canessa, C. M., L. Schild, G. Buell, R. Thorens, I. Gautschi, J. D. Horisberger, and B. C. Rossier. 1994. Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits. Nature 367:463-467.
3. Voilley, N., E. Lingueglia, G. Champigny, M.-G. Mattei, R. Waldmann, M. Lazdunski, and P. Barbry. 1994. The lung amiloride-sensitive Na+ channel: biophysical properties, pharmacology, ontogenesis, and molecular cloning. Proc. Natl. Acad. Sci. USA 91:247-251.
4. McDonald, F. J., P. M. Snyder, P. B. McCray, Jr., and M. J. Welsh. 1994. Cloning, expression and tissue distribution of a human amiloride-sensitive Na+ channel. Am. J. Physiol. 266:L728-L734.
5. McDonald, F. J., M. P. Price, P. M. Snyder, and M. J. Welsh. 1995. Cloning and expression of the beta- and gamma-subunits of the human epithelial sodium channel. Am. J. Physiol. 268:C1157-C1163.
6. Schild, L., E. Schneeberger, I. Gautschi, and D. Firsov. 1997. Identification of amino acid residues in the α, β, and gamma subunits of the epithelial sodium channel (ENaC) involved in amiloride block and ion permeation. J. Gen. Physiol. 109:15-26.
7. Firsov, D., I. Gaulschi, A.-M. Merillat, B. C. Rossier, and L. Schild. 1998. The heterotetrameric architecture of the epithelial sodium channel (ENaC). EMBO J. 17:344-352.
8. Snyder, P. M., C. Cheng, L. S. Prince, J. C. Rogers, and M. J. Welsh. 1998. Electrophysiological and biochemical evidence that DEG/ENaC cation channels are composed of nine subunits. J. Biol. Chem. 273:681-684.
9. Canessa, C. M., A.-M. Merillat, and B. C. Rossier. 1994. Membrane topology of the epithelial sodium channel in intact cells. Am. J. Physiol. 267: C1682-C1690.
10. + channel. J. Biol. Chem. 269:12981-12986.
11. + absorption. Am. J. Physiol. 273:G571-G585.
12. + channel (ENaC) by ubiquitination. EMBO J. 16:6325-6336.
13. Matsushita, K., P. B. McCray, Jr., R. D. Sigmund, M. J. Welsh, and J. B. Stokes. 1996. Localization of epithelial sodium channel subunit mRNAs in adult rat lung by in situ hybridization. Am. J. Physiol. 271:L332-L339.
14. Duc, C., N. Farman, C. M. Canessa, J.-P. Bonvalet, and B. C. Rossier. 1994. Cell-specific expression of epithelial sodium channel α, β, and gamma subunits in aldosterone-responsive epithelia from the rat: localization by in situ hybridization and immunocytochemistry. J. Cell Biol. 127:1907-1921.
15. + channel along rat respiratory tract. Am. J. Physiol. 272:C131-C141.
16. Burch, L. H., C. R. Talbot, M. R. Knowles, C. M. Canessa, B. C. Rossier, and R. C. Boucher. 1995. Relative expression of the human epithelial Na+ channel subunits in normal and cystic fibrosis airways. Am. J. Physiol. 269: C511-C518.
17. Shimkets, R. A., D. G. Warnock, C. M. Bositis, C. Nelson-Williams, J. H. Hansson, M. Schambelan, J. R. J. Gill, S. Ulick, R. V. Milora, J. W. Findling, C. M. Canessa, B. C. Rossier, and R. P. Lifton. 1994, Liddle's syndrome: heritable human hypertension caused by mutations in the beta subunit of the epithelial Na channel. Cell 79:404-414.
18. Chang, S. S., S. Grunder, A. Hanukoglu, A. Rosler, P. M. Mathew, I. Hanukoglu, L. Schild, Y. Lu, R. A. Shimkets, C. Nelson-Williams, B. C. Rossier, and R. P. Lifton. 1996. Mutations in subunits of the epithelial sodium channel cause salt wasting with hyperkalaemic acidosis, pseudohypoaldos-teronism type 1. Nat. Genet. 12:248-253.
19. O'Brodovich, H., V. Hannam, M. Seear, and J. B. Mullen. 1990. Amiloride impairs lung water clearance in newborn guinea pigs. J. Appl. Physiol. 68:1758-1762.
20. Hummler, E., P. Barker, J. Gatzy, F. Beermann, C. Verdumo, A. Schmidt, K. Boucher, and B. C. Rossier. 1996. Early death due to defective neonatal lung liquid clearance in αENaC-deficient mice. Nat. Genet. 12:325-328.
21. Vehaskari, V. M. 1994. Ontogeny of cortical collecting duet sodium transport. Am. J. Physiol. 267:F49-F54.
22. O'Brodovich, H., C. Canessa, J. Ueda, B. Rafii, B. C. Rossier, and J. Edelson. 1993. Expression of the epithelial Na+ channel in the developing rat lung. Am. J. Physiol 265:C491-C496.
23. Dagenais, A., R. Kothary, and Y. Berthiaume. 1997. The α subunit of the epithelial sodium channel of the mouse: developmental regulation of its expression. Pediatr. Res. 42:327-334.
24. Tehepichev, S., J. Ueda, C. Canessa, B. C. Rossier, and H O'Brodovich. 1995. Lung epithelial Na channel subunits are differentially regulated during development and by steroids. Am. J. Physiol. 269:C805-C812.
25. Champigny, G., N, Voilley, H. Lingueglia, V. Friend, P. Barbry, and M. Lazdunski. 1994. Regulation of expression of the lung amiloride-sensitive Na+ channel by steroid hormones. EMBO J. 13:2177-2181.
26. Venkatesh, V. C., and H. D. Katzberg. 1997. Glucocorticoid regulation of epithelial sodium channel genes in human fetal lung. Am. J. Physiol. 273: L227-L233.
27. Sweezey, N., S. Tehepichev, S. Gagnon, K. Fertuck, and H. O'Brodovich. 1998. Female gender hormones regulate mRNA levels and function of the rat lung epithelial Na channel. Am. J. Physiol. 274:C379-C386.
28. Escoubel, B., C. Coureau, J. P. Bonvalet, and N. Farman. 1997. Noncoordinate regulation of epithelial Na channel and Na pump subunit mRNAs in kidney and colon by aldosterone. Am. J. Physiol. 272:C1482-C1491.
29. + channel subunits. Am. J. Physiol. 271: C605-C611.
30. Djelidi, S., M. Fay, F. Cluzeaud, B. Escoubet, E. Eugene, C. Capurro, J. P. Bonvalet, N. Farman, and M. Blot-Chabaud. 1997. Transcriptional regulation of sodium transport by vasopressin in renal cells. J. Biol. Chem. 272: 32919-32924.
31. Yue, G., W. J. Russell, D. J. Benos, R. M. Jackson, M. A. Olman, and S. Matalon. 1995. Increased expression and activity of sodium channels in alveolar type II cells of hyperoxic rats. Proc. Natl. Acad. Sci. USA 92:8418-8422.
32. 2 alters the bioelectric properties of fetal distal lung epithelium. Am. J. Physiol. 270:L1060-L1066.
33. Planès, C., B. Escoubet, M. Blot-Chabaud, G. Friedlander, N. Farman, and C. Clerici. 1997. Hypoxia downregulates expression and activity of epithelial sodium channels in rat alveolar epithelial cells. Am. J. Respir. Cell Mol. Biol. 17:508-518.
34. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1995. Current Protocols in Molecular Biology. John Wiley & Sons, Inc., New York.
35. Tartof, K. D., and C. A. Hobbs. 1988. New cloning vectors and techniques for easy and rapid restriction mapping. Gene 67:169-182.
36. Giguère, V., S. M. Hollenberg, M. G. Rosenfeld, and R. M. Evans. 1986. Functional domains of the human glucocorticoid receptor. Cell 46:645-652.
37. Tini, M., L. C. Tsui, and V. Giguère. 1994. Heterodimeric interaction of the retinoic acid and thyroid hormone receptors in transcriptional regulation on the gamma F-crystallin everted retinoic acid response element. Mol. Endocrinol. 8:1494-1506.
38. Giguère, V., M. Shago, R. Zirngibl, P. Tate, J. Rossant, and S. Varmuza. 1990. Identification of a novel isoform of the retinoic acid receptor gamma expressed in the mouse embryo. Mol. Cell. Biol. 10:2335-2340.
39. Venkatesh, V. C., D. M. Iannuzzi, R. Ertsey, and P. L. Ballard. 1993. Differential glucocorticoid regulation of the pulmonary hydrophobic surfactant proteins SP-B and SP-C. Am. J. Respir. Cell Mol. Biol. 8:222-228.
40. Chomezynski, P., and N. Sacchi. 1987. Single step method of RNA isolation by acid guanidinium-thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
41. Padgett, R. A., P. J. Grabowski, M. M. Konarska, S. Seiler, and P. A. Sharp. 1986. Splicing of messenger RNa precursors. Annu. Rev. Biochem. 55: 1119-1150.
42. Thomas, C. P., N. A. Doggett, R. Fisher, and J. R. Stokes. 1996. Genomic organization and the 5′ flanking region of the gamma subunit of the human amiloride-sensitive epithelial sodium channel. J. Biol. Chem. 271: 26062-26066.
43. Mairbäurl, H., R. Wodopia, S. Eckes, S. Schulz, and P. Bärtsch. 1997. Impairment of cation transport in A549 cells and rat alveolar epithelial cells by hypoxia. Am. J. Physiol. (Lung Cell. Mol. Physiol.) 273:L797-L806.
44. Wodopia, R., P. Bartsch, and H. Mairbäurl. 1997. Hypoxia mediated decrease of sodium transport and sodium channel protein in A549 cells. Pflügers Arch. 433: R44/O-124. (Abstr.)
45. Tsai, M. J., and R. W. O'Malley. 1994. Molecular mechanisms of action of steroid/thyroid receptor superfamily members. Ann. Rev. Biochem. 63: 451-486.
46. Li, X.-J., R.-H. Xu, W. B. Guggino, and S. H. Snyder. 1995. Alternatively spliced forms of the α subunit of the epithelial sodium channel: distinct sites for amiloride binding and channel pore. Mol. Pharmacol. 47:1133-1140.
47. O'Hagan, R. C., R. G. Tozer, M. Symons, F. McCormick, and J. A. Hassell. 1996. The activity of the Ets transcription factor PEA3 is regulated by two distinct MAPK cascades. Oncogene 13:1323-1333.
48. Barker, P. M., M. J. Brown, C. A. Ramsden, L. B. Strang, and D. V. Walters. 1988. The effect of thyroidectomy in the fetal sheep on lung liquid reabsorption induced by adrenaline or cyclic AMP. J. Physiol. (Lond.) 407:373-383.
49. Barker, P. M., M. Markiewicz, A. Parker, D. V. Walters, and L. B. Strang. 1990. Synergistic action of triiodothyronine and hydrocortisone on epinephrine-induced reabsorption of fetal lung liquid. Pediatr. Res. 27:588-591.
50. Pácha, J., I. Pohlová, and Z. Zemanová. 1996. Hypothyroidism affects the expression of electrogenic amiloride-sensitive sodium transport in rat colon. Gastroenterology 111:1551-1557.
51. + channels in A6 cells. Am. J. Physiol. 266:F506-F511.
52. Haskell, J. F., G. Yue, D. J. Benos, and S. Matalon. 1994. Upregulation of sodium conductive pathways in alveolar type II cells in sublethal hyperoxia. Am. J. Physiol. 266:L30-L37.
53. Rafii, B., A. K. Tanswell, G. Otulakowski, O, Pitkanen, R. Belcastro-Taylor, and H. O'Brodovich. 1998. O2-induced ENaC expression is associated with NF-kB activation and blocked by superoxide scavenger. Am. J. Physiol. 275:L764-L770.
54. Otulakowski, G., S. F. Staub, L. Ellis, K. Ramlall, O. Staub, D. Smith, P. Duric, and H. O'Brodovich. 1998. Relation between α, β, gamma hENaC mRNA levels and nasal epithelial potential difference in healthy men. Am. J. Respir. Crit. Care Med. 158:1213-1220.
55. Curtis, D., R. Lehmann, and P. D. Zamore. 1995. Translational regulation in development. Cell 81:171-178.
56. Gu, W., C. Morales, and N. B. Hecht. 1995. In male mouse germ cells, copper-zinc superoxide dismutase utilizes alternative promoters that produce multiple transcripts with different translation potential. J. Biol. Chem. 270: 236-243.
57. Hake, L. E., and N. B. Hecht. 1993. Utilization of an alternative transcription initiation site of somatic cytochrome c in the mouse produces a testisspecific cytochrome c mRNA. J. Biol. Chem. 268:4788-4797.
58. Rao, S. M., and R. D. Howells. 1993. cis-acting elements in the 5′-untranslated region of rat testis proenkephalin mRNA regulate translation of the precursor protein. J. Biol. Chem. 268:22164-22169.
59. Samuels, H. H., F. Stanley, and J. Casanova. 1979. Depletion of L-3,4,3′-triiodothyronine and L-thyroxine in euthyroid calf serum for use in cell culture studies of the action of thyroid hormone. Endocrinology 105:80-85.