Pseudomonas aeruginosa internalization by human epithelial respiratory cells depends on cell differentiation, polarity, and junctional complex integrity

American Journal of Respiratory Cell and Molecular Biology

Volumn 20, Issue 5, 1999, Pages 880-890

  Plotkowski, Maria Cristina ^a   De Bentzmann, Sophie ^b   Pereira, Silvia Helena M ^b   Zahm, Jean Marie ^b   Bajolet Laudinat, Odile ^b   Roger, Patricia ^b   Puchelle, Edith ^b  

^a STATE UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

CFTR PROTEIN, HUMAN; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ARTICLE; BRONCHUS; CELL CULTURE; CELL DIFFERENTIATION; CELL POLARITY; CYTOLOGY; ELECTRON MICROSCOPY; EPITHELIUM CELL; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; METABOLISM; MICROBIOLOGY; PHYSIOLOGY; PSEUDOMONAS AERUGINOSA; ULTRASTRUCTURE;

BRONCHI; CELL DIFFERENTIATION; CELL POLARITY; CELLS, CULTURED; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; EPITHELIAL CELLS; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; MICROSCOPY, ELECTRON; PSEUDOMONAS AERUGINOSA;

EID: 0033124228     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/ajrcmb.20.5.3408     Document Type: Article

References (38)

1. de Bentzmann, S., M. C. Plotkowski, and E. Puehelle. 1996. Receptors in the Pseudomonas aeruginosa adherence to injured and repairing airway epithelium. Am. J. Respir. Crit. Care Med. 154:S155-S162.
2. Puchelle, E., D. Gaillard, D. Ploton, J. Hinnrasky, C. Fuchey, M. C. Bout terin, J. Jacquol, and D. Dreyer. 1992. Differential localization of the cystic fibrosis transmembrane conductance regulator in normal and cystic fibrosis airway epithelium. Am. J. Respir. Cell Mol. Biol. 7:485-491.
3. Gaillard, D. A., S. Ruocco, A. Lallemand, W. Dalemans, J. Hinnrasky, and E. Puchelle. 1994. Immunohistochemical localization of cystic fibrosis transmembrane conductance regulator in human fetal airway and in digestive tissue. Pediatr. Res. 36:137-143.
4. Cheng, S. H., R. J. Gregory, J. Marshal, S. Paul, D. Souza, G. A. White, C. R. O'Riordan, and A. E. Smith. 1990. Defective intracellular transport and processing of CFTK is the molecular basis of most cystic fibrosis. Cell 63:827-834.
5. Dupuit, F., N. Kalin, S. Brézillon, J. Hinnrasky, B. Tummler, and E. Puchelle. 1995. CFTR and differentiation markers expression in non-CF and ΔF 508 homozygous CF nasal epithelium. J. Clin. Invest. 96:1601-1611.
6. Pier, G. B., M. Grout, T. S. Zaidi, J. C. Olsen, J. R. Yankaskas, and J. B. Goldberg. 1996. Role of mutant CFTR in hypersusceplibility of cystic fibrosis patients to lung infection. Science 271:64-67.
7. Pier, G. B., M. Gout, and T.S. Zaidi. 1997. Cystic fibrosis Transmembrane conductance is an epithelial cell receptor for clearance of Pseudomonas earuginosa from the lung. Proc. Natl Acad. Sci. USA 94:12088-12093.
8. Tang, H., M. Kays, and A. Prince. 1995. Role of Pseudomonas aeruginosa pili in acute pulmonary infection. Infect. Immun. 63:1278-1285.
9. DiMango, E., R. Bryan, and A. Prince. 1996. Epithelial ingestion of Pseudmnonas aeruginosa: a comparison of CF and normal cells in primary culture and transformed cell lines. Pediatr. Pulmonol. 13:287. (Abstr.)
10. Ko, Y. H., M. Delannoy, and P. L. Pedersen. 1997. Cystic fibrosis, lung infection and a human tracheal antimicrobial peptide (hTAP). FEBS Lett. 405:200-208.
11. Plotkowski, M. C., O. Bajolet-Laudinat, and E. Puchelle. 1993. Cellular and molecular mechanisms of bacterial adhesion to respiratory mucosa. Eur. Respir. J. 6:903-916.
12. Pereira, S. H. M., M. P. Cervante, S. De Bentzmann, and M. C. Plotkowski. 1997. Pseudomonas aeruginosa entry into Caco-2 cells is enhanced in repairing wounded monolayers. Microb. Pathog. 23:249-255.
13. Molitoris, B. A., and W. J. Nelson. 1990. Alterations in the establishment and maintenance of epithelial cell polarity as a basis for disease processes. J. Clin. Invest. 85:3-9.
14. Gordon, R. E., and B. P. Lane. 1976. Regeneration of rat tracheal epithelium afer mechanical injury: restoration of surface integrity during the early hours after injury. Am. Rev. Respir. Dis. 113:799-807.
15. Herard, A. L., J. M. Zahm, D. Pierrot, H. Hinnrasky, C. Fuchey, and E. Puchelle. 1996. Epithelial barrier integrity during in vitro wound repair of the airway epithelium. Am. J. Respir. Cell Mol. Biol. 15:624-632.
16. Herard, A. L., D. Pierrot, J. Hinnrasky, H. Kaplan, D. Sheppard, E. Puchelle, and J. M. Zahm. 1996. Fibronectin and α5β1 receptor are involved in the wound repair process of the airway epithelium. Am. J. Physiol. (Lung Cell. Mol. Physiol.) 271:L726-L733.
17. Fleizig, S. M. J., D. J. Evans, N. Do, V. Vallas, S. Shin, and K. E. Mostov. 1997. Epithelial cell polarity affects susceptibility to Pseudomonas aeruginosa invasion and eytotoxicity. Infect. Immun. 65:2861-2867.
18. Cozzens, A. L., M. J. Yezzi, M. Yamada, D. Steiger, J. A. Wagner, S. S. Garber, L. Chin. E. M. Simon, G. R. Cutting, P. Gardner, D. S. Friend, C. B. Basbaum, and D. C. Gruenert. 1992. Transformed human epithelial cell line that retains light junctions post crisis. In Vitro Cell Dev. Biol. (Anim.) 28A:735-744.
19. Chevillard, M., J. Hinnrasky, D. Pierrot, J. M. Zahm, J. M. Klossek, and E. Puchelle. 1993. Differentiation of human surface upper airway epithelial cells in primary culture on a floating collagen gel. Epithelial Cell Biol. 2:17-25.
20. Chambard, M., J. Gabrion, and J. Mauchamp. 1981. Influence of collagen gel on the orientation of epithelial cell polarity: follicule formation from isolated thyroid cells and preformed monolayers. J. Cell Biol. 91:157-166.
21. Shaklai, M., and M. Tavassoli. 1982. Lanthanum as an electron microscopic stain. J. Histochem. Cytochem. 30:1325-1330.
22. Revel, J. P., and M. J. Karnovsky. 1967. Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol. 33:C7-C12.
23. Ishimoto, K. S., and S. Lory. 1989. Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (Rpon) of RNA polymerase. Proc. Natl. Acad. Sci. USA 86:1954-1957.
24. Denizot, E., and R. Lang. 1986. Rapid colorimetric assay for cell growth and survival: modification to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods. 89:271-275.
25. Mitchell, D. B., K. S. Santone, and D. Acosta. 1980. Evaluation of cytotoxicity in cultured cells by enzyme leakage. J. Tissue Culture Methods 6:113-121.
26. Falk, P., T. Boren, D. Haslam, and M. Caparon. 1994. Bacterial adhesion and colonization assays. In Microbes As Tools for Cell Biology. D. G. Russel, editor. Academic Press, San Diego. 165-192.
27. Gruenerl, D. C., W. E. Finkbeiner, and J. H. Widdicombe. 1995. Culture and transformation of human airway epithelial cells. Am. J. Physiol. 268: L347-L360.
28. Plotkowski, M. C., M. Chevillard, D. Pierrot, D. Altemuyer, J. M. Zahm, G. Colliot, and E. Puehelle. 1991. Differential adhesion of Pseudomonas aeruginosa to human respiratory epithelial cells in primary culture. J. Clin. Invest. 87:2018-2028.
29. Plotkowski, M. C., J. M. Zahm, and E. Puehelle. 1992. Pseudomonas aeruginosa adhesion to normal and injured respiratory mueosa. Mem. Inst. Oswaldo Cruz 87(Suppl.):61-68.
30. Girod de Bentzmann, S., O. Bajolet-Laudinat, F. Dupuit, D. Pierrot, C. Fuchey, M. C. Plutkowski, and E. Puehelle. 1994. Protection of human respiratory epithelium from Pseudomonas aeruginosa adherenee by disteroyl phosphatidylglycerol liposomes. Infect. Immun 62:70.1-708.
31. Stern, G. A., A. Lubniewski, and C. Allen. 1985. The interaction between Pseudomonas aeruginosa and the corrneal epithelium: an electron microscopic study. Arch. Ophthatmol. 103:1221-1225.
32. Mounier, J., T. Vasselon, R. Hellio, M. Lesouird, and P. J. Sansonetti. 1992. Shigella flexneri enters human colonie Caco-2 epithelial cells through the basolateral pole. Infect. Immun. 60:237-248.
33. Perdomo, J. J., P. Gounon, and P. J. Sansonetti. 1994. Polymorphonuclear leukocyte transmigration promotes invasion of colonic epithelial monolayer by Shigella flexneri. J. Clin. Invest. 93:633-643.
34. Gaillard, J. L., and B. B. Finlay. 1996. Effect of cell polarization and differentiation on entry of Listeria monocytigenes into the enterocyte-like Caco-2 cell line. Infect. Immun. 64:1299-1308.
35. McCormiek, B. A., A. Nusrat, C. A. Parkos, Lisa D'Andrea, P. M. Hofman, D. Carnes, T. W. Liang. and J. L. Madara. 1997. Unmasking of intestinal epithelial lateral membrane β1 integrin consequent to transepithelial neuirophil migration in vitro facilitates inv-mediated invasion by Versinia pseudotuberculosis. Infect. Immun. 65:1414-1421.
36. Plotkowski, M. C., and M. N. Meirelles. 1997. Concomitant endosome-phagosome fusion and lysis of endosomal membranes account for Pseudomonas. aeruginosa survival in human endothelial cells. J. Submicrosc. Cytol. Pathol. 29:229-237.
37. Horwitz, M. A. 1983. Formation of a novel phagosome by the Legionnaire's disease bacterium (Legionella pneuntophila) in human monocytes. J. Exp. Med. 158:1319-1331.
38. Melo, E. J. T., T. U. De Carvalho, and W. De Souza. 1992. Penetration of Toxoplasma gondii into host cells induces changes in the distribution of the mitochondria and the endoplasmic reticulum. Cell Stract. Funct. 17: 311-117.