Restriction fragment-length polymorphism analysis of 16S ribosomal RNA genes amplified by polymerase chain reaction for rapid identification of cultivable oral treponemes

Oral Microbiology and Immunology

Volumn 14, Issue 2, 1999, Pages 117-121

  Sato, T ^a   Kuramitsu, Howard K ^a  

^a UNIVERSITY AT BUFFALO   (United States)

Author keywords

16S rRNA; Oral treponeme; PCR RFLP

Indexed keywords

BACTERIUM IDENTIFICATION; CHROMOSOME BANDING; ENDONUCLEASE; GENE SEQUENCING; POLYMERASE CHAIN REACTION; PRIMER DNA; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RIBOSOMAL RNA;

BACTERIAL TYPING TECHNIQUES; GENES, BACTERIAL; MOUTH; PERIODONTAL POCKET; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RNA, RIBOSOMAL, 16S; TREPONEMA;

EID: 0033120416     PISSN: 09020055     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-302X.1999.140207.x     Document Type: Article

References (37)

1. Barron SL, Riviere GR, Simonson LG, Lukehart SA, Tira DE, O'Neil DW. Use of monoclonal antibodies to enumerate spirochetes and identify Treponema denticola in dental plaque of children, adolescents and young adults. Oral Microbiol Immunol 1991: 6: 97-101.
2. Chan ECS, Siboo R, Keng T, Psarra N, Hurley R, Cheng SL, Iugovaz I. Treponema denticola (ex Brumpt 1925) sp. nov., nom. rev., and identification of new spirochete isolates from periodontal pockets. Int J Syst Bacteriol 1993: 43: 196-203.
3. Chan ECS, Klitorinos A, Gharbia S, Caudry SD, Rahal MD, Siboo R. Characterization of a 4.2-kb plasmid isolated from periodontopathic spirochetes. Oral Microbiol Immunol 1996: 11: 365-368.
4. Choi BK, Paster BJ, Dewhirst FE, Göbel UB. Diversity of cultivable and uncultivable oral spirochetes from a patient with severe destructive periodontitis. Infect Immun 1994: 62: 1889-1895.
5. Dahle UR, Tronstad L, Olsen I. Spirochaetes in oral infections. Endod Dent Traumatol 1993: 9: 87-94.
6. Dahle UR, Olsen I, Tronstad L, Caugant DA. Population genetic analysis of oral treponemes by multilocus enzyme electrophoresis. Oral Microbiol Immunol 1995: 10: 265-270.
7. Dahle UR, Tronstad L, Olsen I. Characterization of new periodontal and endodontic isolates of spirochetes. Eur J Oral Sci 1996: 104: 41-47.
8. DiRienzo JM, Cornell S, Boehringer H. Use of randomly cloned DNA fragments for the identification of oral spirochetes. Oral Microbiol Immunol 1991: 6: 88-96.
9. Ericson RL, Lau CN, Levanos VA, Paster BJ, Dewhirst FE. There are at least forty species of human periodontal spirochetes. J Dent Res 1998: 77: 139 (abstr 267).
10. Fiehn N-E, Bangsborg JM, Colding H. Ribotyping on small-sized spirochetes isolated from subgingival plaque. Oral Microbiol Immunol 1995: 10: 13-18.
11. Fukumoto Y, Okuda K, Kato T, Ohta K, Takazoe I. Taxonomic study of spirochetes isolated from periodontal lesions. Oral Microbiol Immunol 1987: 2: 82-87.
12. Fukumoto Y, Kato T, Ishihara K, Seida K, Takazoe I, Okuda K. A common antigen of Treponema denticola and other Treponema species detected by monoclonal antibody. Oral Microbiol Immunol 1989: 4: 112-116.
13. Kearns EA, Simonson LG, Schutt RW, Johnson MJ, Neil LC. Characterization of monoclonal antibodies to two Treponema denticola serotypes by the indirect fluorescent-antibody assay. Microbios 1991: 65: 147-153.
14. Koseki T, Ishikawa I, Umeda M, Benno Y. Characterization of oral treponemes isolated from human periodontal pockets. Oral Microbiol Immunol 1995: 10: 271-277.
15. Laughon BE, Syed SA, Loesche WJ. API ZYM system for identification of Bacteroides spp., Capnocytophaga spp., and spirochetes of oral origin. J Clin Microbiol 1982: 15: 97-102.
16. Loesche WJ. The role of spirochetes in periodontal disease. Adv Dent Res 1988: 2: 275-283.
17. Miller JN, Smibert RM, Norris SJ. The genus Treponema. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H, ed. The prokaryotes. 2nd edn. New York: Springer-Verlag, 1992: 3537-3559.
18. Moore WEC, Moore LVH. The bacteria of periodontal diseases. Periodontol 2000 1994: 5: 66-77.
19. Nakamura Y, Umemoto T, Nakatani Y, Namikawa I, Wadood A. Common and specific antigens of several treponemes detected by polyclonal antisera against major cellular proteins. Oral Microbiol Immunol 1993: 8: 288-294.
20. Olsen I, Fiehn N-E, Johnson JL. Identity of 1:2:1 and 2:4:2 subgingival spirochetes by DNA hybridization. Oral Microbiol Immunol 1995: 10: 378-381.
21. Parrish KD, Greenberg EP. A rapid method for extraction and purification of DNA from dental plaque. Appl Environ Microbiol 1995: 61: 4120-4123.
22. Paster BJ, Dewhirst FE, Coleman BC, Lau CN, Ericson RL. Phylogenetic analysis of cultivable oral treponemes from the Smibert collection. Int J Syst Bacteriol (in press).
23. Paster BJ, Dewhirst FE, Cooke SM, Fussing V, Poulsen LK, Breznak JA. Phylogeny of not-yet-cultured spirochetes from termite guts. Appl Environ Microbiol 1996: 62: 347-352.
24. Riviere GR, Smith KS, Carranza N Jr, Tzagaroulaki E, Kay SL, Dock M. Subgingival distribution of Treponema denticola, Treponema socranskii, and pathogen-related oral spirochetes: prevalence and relationship to periodontal status of sampled sites. J Periodontol 1995: 66: 829-837.
25. Sato T, Matsuyama J, Sato M, Hoshino E. Differentiation of Veillonella atypica, Veillonella dispar and Veillonella parvula using restricted fragment-length polymorphism analysis of 16S rDNA amplified by polymerase chain reaction. Oral Microbiol Immunol 1997: 12: 350-353.
26. Sato T, Matsuyama J, Takahashi N, Sato M, Johnson J, Schachtele C, Hoshino E. Differentiation of oral Actinomyces species by 16S rDNA polymerase chain reaction-restriction fragment length polymorphism. Arch Oral Biol 1998: 43: 247-252.
27. Sato T, Sato M, Matsuyama J, Kalfas S, Sundqvist G, Hoshino E. Restriction fragment-length polymorphism analysis of 16S rDNA from oral asaccharolytic Eubacterium species amplified by polymerase chain reaction. Oral Microbiol Immunol 1998: 13: 23-29.
28. Sela MN, Kornman KS, Ebersole JL, Holt SC. Characterization of treponemes isolated from human and non-human primate periodontal pockets. Oral Microbiol Immunol 1987: 2: 21-29.
29. Smibert RM. Burmeister JA. Treponema pectinororum sp. nov. isolated from humans with periodontitis. Int J Syst Bacteriol 1983: 33: 852-856.
30. Smibert RM, Johnson JJ, Ranney RR. Treponema socranskii sp. nov., Treponema socranskii subsp. socranskii subsp. nov., Treponema socranskii subsp. buccale subsp. nov., and Treponema socranskii subsp. paredis subsp. nov. isolated from the human periodontia. Int J Syst Bacteriol 1984: 34: 457-462.
31. Smibert RM. Genus Treponema. In: Krieg NR, Holt JG, ed. Bergey's manual of systematic bacteriology. Baltimore: Williams & Wilkins, 1984: 49-57.
32. Tanner A, Lai C-H, Maiden M. Characteristics of oral gram-negative species. In: Slots J, Taubman MA, ed. Contemporary oral microbiology and immunology. St. Louis: Mosby-Year Book. 1992: 299-341.
33. Tanner A, Maiden MFJ, Paster BJ, Dewhirst FE. The impact of 16S ribosomal RNA-based phylogeny on the taxonomy of oral bacteria. Periodontol 2000 1994: 5: 26-51.
34. Umemoto T, Nakazawa F, Hoshino E, Okada K, Fukunaga M, Namikawa I. Treponema medium sp. nov. isolated from subgingival dental plaque. Int J Syst Bacteriol 1997: 47: 67-72.
35. Westergaard, Fiehn N-E. Morphological distribution of spirochetes in subgingival plaque from advanced marginal periodontitis in humans. APMIS 1987: 95: 49-55.
36. Wyss C, Choi BK, Schüpbach P, Guggenheim B, Göbel UB. Treponema maltophilum sp. nov. a small oral spirochete isolated from human periodontal lesions. Int J Syst Bacteriol 1996: 46: 745-752.
37. Wyss C, Choi BK, Schüpbach P, Guggenheim B, Gobel UB. Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int J Syst Bacteriol 1997: 47: 842-845.