Towards elucidation of microbial community metabolic pathways: Unravelling the network of carbon sharing in a pollutant-degrading bacterial consortium by immunocapture and isotopic ratio mass spectrometry

Environmental Microbiology

Volumn 1, Issue 2, 1999, Pages 167-174

  Pelz, Oliver ^a,b   Tesar, Michael ^a   Wittich, Rolf Michael ^a   Moore, Edward R B ^a   Timmis, Kenneth N ^a   Abraham, Wolf Rainer ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS);

CARBON; FURAN DERIVATIVE; PROTOANEMONIN; SALICYLIC ACID DERIVATIVE;

ALCALIGENES; ARTICLE; BACTERIUM; CLASSIFICATION; CULTURE MEDIUM; ECOSYSTEM; ENZYME LINKED IMMUNOSORBENT ASSAY; GROWTH, DEVELOPMENT AND AGING; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; POLLUTANT; PSEUDOMONAS;

ALCALIGENES; BACTERIA; CARBON; CARBON ISOTOPES; CULTURE MEDIA; ECOSYSTEM; ENVIRONMENTAL POLLUTANTS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FURANS; MASS SPECTROMETRY; PSEUDOMONAS; SALICYLATES;

EID: 0033108071     PISSN: 14622912     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-2920.1999.00023.x     Document Type: Article

References (18)

1. Abraham, W.R., Meyer, H., Lindholst, S., Vancanneyt, M., and Smit, J. (1997) Phospho- and sulfolipids as biomarkers of Caulobacter, Brevundimonas and Hyphomonas. Syst Appl Microbiol 20: 522-539.
2. Abraham, W.R., Hesse, C., and Pelz, O. (1998) Isotopic ratios of carbon in microbial lipids as indicator of substrate usage. Appl Environ Microbiol 64: 4202-4209.
3. 12C ratios in individual fatty acids of marine mytilids with and without bacterial symbionts. Org Geochem 12: 611-617.
4. Armengaud, J., Happe, B., and Timmis, K.N. (1998) Genetic analysis of dioxin dioxygenase of Sphingomonas sp. strain RW1: catabolic genes dispersed on the genome. J Bacteriol 180: 3954-3966.
5. Blasco, R., Wittich, R.M., Mallavarapu, M., Timmis, K.N., and Pieper, D.H. (1995) From xenobiotic to antibiotic. Formation of protoanemonin from 4-chlorocatechol by enzymes of the 3-oxoadipate pathway. J Biol Chem 49: 29229-29235.
6. Blasco, R., Mallavarapu, M., Wittich, R.M., Timmis, K.N., and Pieper, D.H. (1997) Evidence that the formation of protoanemonin from metabolites of 4-chlorobiphenyl degradation negatively affects the survival of 4-chlorobi-phenyl-cometabolizing microorganisms. Appl Environ Microbiol 63: 427-434.
7. Brückmann, M., Blasco, R., Timmis, K.N., and Pieper, D.H. (1998) Detoxification of protoanemonin by dienelactone hydrolase. J Bacteriol 180: 400-402.
8. Chapman, P.A., Malo, A.T., Siddons, C.A., and Harkin, M. (1997) Use of commercial enzyme immunoassays and immunomagnetic separation systems for detecting Escherichia coli 0157 in bovine fecal samples. Appl Environ Microbiol 63: 2549-2553.
9. Didry, N., Dubreuil, L., and Pinkas, M. (1991) Antibacterial activity of vapors of protoanemonin. Pharmazie 46: 546-547.
10. Faude, U.C. (1995) Immunchemische Analyse mikrobieller Lebensgemeinschaften. PhD Thesis, University of Leipzig, Germany.
11. Gibson, D.T., and Subramanian, V. (1984) Microbial degradation of aromatic hydrocarbons. In Microbial Degradation of Organic Compounds. Gibson, D.T. (ed.). New York: Dekker, pp. 181-252.
12. Harms, H., Wilkes, H., Sinnwell, V., Wittich, R.M., Figge, K., Francke, W., et al. (1991) Transformation of 3-chlorobenzofuran by Pseudomonas sp. HH 69. FEMS Microbiol Lett 65: 25-29.
13. Mau, M., and Timmis, K.T. (1998) Use of subtractive hybridization to design habitat-based oligonucleotide probes for investigation of natural bacterial communities. Appl Environ Microbiol 64: 185-191.
14. Morris, C.M., and Barnsley, E.A. (1982) The cometabolism of 1- and 2-chloronaphthalene by pseudomonads. Can J Microbiol 28: 73-79.
15. Pelz, O., Hesse, C., Tesar, M., Coffin, R.B., and Abraham, W.R. (1997) Development of methods to measure carbon isotope ratios of bacterial biomarkers in the environment. Isotopes Environ Health Stud 33: 131-144.
16. Seegal, B.C., and Holden, M. (1945) The antibiotic activity of extracts of Ranunculaceae. Science 101: 413-414.
17. Tamura, G.S., Dailey, M.O., Gallatin, M.W., McGrath, M.S., Weissman, I.L., and Pillemer, E.A. (1984) Isolation of molecules by monoclonal antibodies and antisera: the solid phase immunoisolation technique. Anal Biochem 136: 458-464.
18. White, D.C., Davis, W.M., Nickels, J.S., King, J.D., and Bobbie, R.B. (1979) Determination of the sedimentary microbial biomass by extractable lipid phosphate. Oecologia 40: 51-62.