Heat pretreatment increases resolution in DNA flow cytometry of paraffin-embedded tumor tissue

Cytometry

Volumn 35, Issue 3, 1999, Pages 260-266

  Leers, M P G ^a   Schutte, B ^b   Theunissen, P H M H ^a   Ramaekers, F C S ^b   Nap, M ^a  

^a Sittard Geleen Heerlen   (Netherlands)

^b MAASTRICHT UNIVERSITY   (Netherlands)

Author keywords

Cell cycle analysis; High resolution flow cytometry; Immunochemistry; Paraffin embedded tissue; Ploidy estimation

Indexed keywords

CITRIC ACID; CYTOKERATIN; FORMALDEHYDE; PARAFFIN; PROPIDIUM IODIDE;

ARTICLE; CANCER TISSUE; DNA CONTENT; FLOW CYTOMETRY; HEATING; HISTOLOGY; HUMAN; HUMAN TISSUE; PLOIDY; PRIORITY JOURNAL;

BREAST NEOPLASMS; CELL SEPARATION; COLON; COLORECTAL NEOPLASMS; DOSE-RESPONSE RELATIONSHIP, DRUG; FLOW CYTOMETRY; FORMALDEHYDE; HEAT; HUMANS; IMMUNOHISTOCHEMISTRY; KERATINS; KINETICS; MENINGEAL NEOPLASMS; NEOPLASMS; PARAFFIN EMBEDDING; PEPSIN A; PROPIDIUM; TIME FACTORS;

EID: 0033105071     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19990301)35:3<260::AID-CYTO9>3.0.CO;2-O     Document Type: Article

References (24)

1. Vindeløv LL, Christensen IJ. A review of techniques and results obtained in one laboratory by an integrated system of methods designed for routine clinical flow cytometric DNA analysis. Cytometry 1990;11:753-770.
2. Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA. Method for analysis of cellular DNA content of paraffin embedded pathological material using flow cytometry. J Histochem Cytochem 1983;31:1333-1335.
3. Bauer KD, Bagwell B, Giaretti W, Melamed M, Zarbo RJ, Witzig TE, Rabinovitch PS. Consensus review of the clinical utility of DNA flow cytometry in colorectal cancer. Cytometry 1993;14:486-491.
4. Duque RE, Andreeff M, Braylan RC, Diamond LW, Peiper SC. Consensus review of the clinical utility of DNA flow cytometry in neoplastic hematopathology. Cytometry 1993;14:492-496.
5. Hedley DW. Flow cytometry using paraffin-embedded tissue: live years on. Cytometry 1989;10:229-241.
6. Hedley DW, Clark GM, Cornellise CJ, Killander D, Kute T, Merkel D. Consensus review of the clinical utility of DNA cytometry in carcinoma of the breast Cytometry 1993;14:482-485.
7. Shankey TV, Kallioniemi OP, Koslowski JM, Lieber ML, Mayall BH, Miller G, Smith GJ. Consensus review of the clinical utility of DNA content cytometry in prostate cancer. Cytometry 1993;14:497-500.
8. Shankey TV, Rabinovitch PS, Bagwell B, Bauer KD, Duque RE, Hedley DW, Mayall BH, Wheeles L. Guidelines for implementation of clinical DNA cytometry. Cytometry 1993;14:472-477.
9. Frei JV, Martinez J. DNA flow cytometry of fresh and paraffin-embedded tissue using cytokeratin staining. Mod Pathol 1993;6:599-605.
10. Leers MPG, Theunissen PHMH, Schutte B, Ramaekcrs FCS. Bivariate cytokeratin/DNA flow cytometric analysis of paraffin embedded samples from colorectal carcinomas. Cytometry 1995;21:101-107.
11. Schultz DS, Zarbo RJ. Comparison of eight modifications of Hedley's method for flow cytometric DNA ploidy analysis of paraffin-embedded tissue. Am J Clin Pathol 1992;98:291-295.
12. Schutte B, Reynders MM, Bosman FT, Blijham GT. Flow cytometric determination of DNA ploidy level in nuclei isolated from paraffin-embedded tissue. Cytometry 1985;6:26-30.
13. Leers MPG, Theunissen PHMH, Ramaekers FCS, Schutte B. Multiparameter flow cytometric analysis with detection of the Ki67-Ag in paraffin embedded mammary carcinomas. Cytometry 1997;27:283-289.
14. Cattoretti G, Suurmeijer AJH. Antigen unmasking on fromalin-fixed, paraffin embedded tissues using microwaves: a review. Adv Anat Pathol 1995;2:2-9.
15. Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin fixed, paraffin embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991;39:741-744.
16. Werner M, Wasielewski von R, Komminoth P. Antigen retrieval, signal amplification and intensification in immunohistochemistry. Histochem Cell Biol 1996;105:253-260.
17. Hasellkorn R, Doty P. The reaction of formaldehyde with polynucleotides. J Biol Chem 1961;236:2738-2745.
18. Overton WR, Catalano E, McCoy JP. Method to make paraffin-embedded breast and lymph tissue mimic fresh tissue in DNA analysis. Cytometry 26: 166-171, 1996
19. Beisker W, Dolbaere F, Gray JW. An improved immunocytochemical procedure for high-sensitivity detection of incorporated bromodeoxyuridine. Cytometry 1987;8:235-239.
20. Dolbaere F, Gray JW. Use of restriction endonucleases and exonuclease III to expose halogenated pyrimidines for immunochemical staining. Cytometry 1988;9:631-635.
21. Morgan JM, Navabi H, Schmid KW Jasani B. Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval. J Pathol 1994;147:301-307.
22. Artlich A, Schmidt D. Immunohistochemical profile of meningeomas and their histological subtypes. Hum Pathol 1990;21:843-849.
23. Arber DA, Cook PD, Moser LK, Speights VO. Variation in reference cells for DNA analysis of paraffin embedded tissue. Am J Clin Pathol 1992;97:387-392.
24. Overton WR, McCoy JP. Reversing the effect of formalin on the binding of propidium iodide to DNA. Cytometry 1994;16:351-356.