Reverse transcription polymerase chain reaction method for the detection of glycopeptide resistance in enterococci

Journal of Microbiological Methods

Volumn 35, Issue 2, 1999, Pages 95-100

  Privitera, Orazio ^a   Sisto, Francesca ^b   Giuffrida, Vincenza ^b   Puntorieri, Miriam ^a   Cascone, Carmela ^a   Silvestro, Isabella Di ^b   Rappazzo, Giancarlo ^a   Stefani, Stefania ^a  

^a UNIVERSITY OF CATANIA   (Italy)

^b AID Research Center   (Italy)

Author keywords

Enterococcus; Glycopeptide; Mechanisms of resistance; RT PCR

Indexed keywords

GLYCOPEPTIDE; MESSENGER RNA; TEICOPLANIN; VANCOMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; ENTEROCOCCUS; GENE EXPRESSION; MINIMUM INHIBITORY CONCENTRATION; PHENOTYPE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; TECHNIQUE;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CARBON-OXYGEN LIGASES; DRUG RESISTANCE, MICROBIAL; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; GENE EXPRESSION; MICROBIAL SENSITIVITY TESTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TEICOPLANIN; VANCOMYCIN;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS;

EID: 0033105069     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(98)00096-7     Document Type: Article

References (14)

1. Arthur, M., Courvalin, P., 1993. Genetic and mechanism of glycopeptide resistance in enterococci. Antimicrob. Agents Chemother. 37, 1563-1571.
2. Clark, N.C., Cooksay, R.C., Hill, B.C., Swenson, J.M., Tenover, F.C., 1993. Characterization of glycopeptide-resistant enterococci from US hospitals. Antimicrob. Agents Chemother. 37, 2311-2317.
3. Courvalin, P., 1990. Resistance of enterococci to glycopeptides. Antimicrob. Agents Chemother. 34, 2291-2296.
4. Dukta-Malen, S., Molinas, C., Arthur, M., Courvalin, P., 1990. The Van A glycopeptide resistance protein is related to D-alanyl-D-alanine ligase cell wall biosynthesis enzymes. Gen. Genet. 224, 364-372.
5. Th Interscience Conference of Antimicrobial Agents and ChemoTherapy (ICAAC), abs E34, pp. 91.
6. Hayden, M.K., Trenholme, G.M., Schultz, J.E., Sahm, D.F., 1993. In vivo development of teicoplanin resistance in a Van B Enterococcus faecium. J. Infect. Dis. 167, 1224-1227.
7. Th Mediterranean Congress of ChemoTherapy (MCC), Italy, abs 028, pp. 11.
8. Persing, D.H., Smith, T.F., Tenover, F.C., White, T.J., 1993. Diagnostic Molecular Microbiology (American Society for Microbiology, Ed.), pp. 519-553.
9. Pitcher, D.G., Saunders, N.A., Owen, R.J., 1989. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol. 8, 151-156.
10. Quintiliani, R.Yr., Evers, S., Courvalin, P., 1993. The vanB gene confers various levels of self-transferable resistance to vancomycin in enterococci. J. Infect. Dis. 167, 1220-1223.
11. Sambrook, J., Fritsch, E.F., Maniats, T., 1989. Molecular Cloning: A Laboratory Manual, Second Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
12. Tenover, F.C., Tokars, J., Swenson, J., Paul, S., Spitalny, K., Jarvis, W., 1993. Ability of clinical laboratories to detect antimicrobial agent-resistant enterococcci. J. Clin. Microbiol. 31, 1695-1699.
13. Tomasz, A., 1994. Multiple antibiotic-resistant pathogenic bacteria: A report on the Rockfeller University workshop. New Engl. J. Med. 330, 1247-1251.
14. Woodford, N., Morrison, D., Johnson, A.P., Briant, V., George, R.C., Cookson, B., 1993. Application of DNA probes for rRNA and vanA genes to investigation of a nosocomial cluster of vancomycin-resistant enterococci. J. Clin. Microbiol. 31, 653-658.