Application of a fed-batch system to produce RNA by in vitro transcription

Biotechnology Progress

Volumn 15, Issue 2, 1999, Pages 174-184

  Kern, Jeffrey A ^a,b   Davis, Robert H ^a  

^a University of Colorado at Boulder   (United States)

^b GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FED BATCH REACTOR; IN VITRO STUDY; MAGNESIUM; NUCLEOSIDE TRIPHOSPHATE; RNA POLYMERASE; RNA SYNTHESIS;

BATCH CELL CULTURE; BIOREACTORS; DNA; ENZYMES; MAGNESIUM COMPOUNDS; PH EFFECTS; PHOSPHORUS COMPOUNDS; RNA; SALTS;

FED-BATCH CELL CULTURE; NUCLEOSIDE TRIPHOSPHATE; POLYMERASE; STIRRED-CELL REACTORS;

GENETIC ENGINEERING;

ACID ANHYDRIDE HYDROLASES; BIOREACTORS; CELL CULTURE TECHNIQUES; COSTS AND COST ANALYSIS; HYDROGEN-ION CONCENTRATION; NUCLEOSIDE-TRIPHOSPHATASE; RNA; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION, GENETIC;

EID: 0033104169     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp990008g     Document Type: Article

References (18)

1. Cech, T. R. The chemistry of self-splicing RNA and RNA enzymes. Science 1987, 236, 1532-1539.
2. Tuerk, C.; Gold, L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 1990, 249, 505-510.
3. Ellington, A. D.; Szostak, J. W. In vitro selection of RNA molecules that bind specific ligands. Nature 1990, 346, 818.
4. Uhlmann, E.; Peyman, A. Anti-sense oligonucleotides: a new therapeutic principle. Chem. Rev. 1990, 90, 544-584.
5. Pfleiderer, W.; Matysiak, S.; Bergmann, F.; Schnell, R. Recent progress in oligonucleotide synthesis. Acta Biochim. Pol. 1996, 43, 37-44.
6. Pieken, W. Efficient process technologies for the preparation of oligonucleotides. Ciba Found. Symp. 1997, 209, 218-223.
7. Davis, R. H. Large-scale oligoribonucleotide production. Curr. Opin. Biotechnol. 1995, 6, 213-217.
8. Marble, A. H.; Davis, R. H. RNA transcription from immobilized DNA templates. Biotechnol. Prog. 1895, 11, 393-396.
9. Vant-Hull, B. The binding of T7 RNA polymerase to double-stranded DNA: strategies for large-scale transcription. Ph.D. dissertation, University of Colorado, Boulder, CO, 1997.
10. Davis, R. H.; Breckenridge, N. C. Modeling of repeated-batch transcription for production of RNA. J. Biotechnol. 1999, in press.
11. Martin, C. T.; Muller, D. K.; Coleman, J. E. Processivity in early stages of transcription by T7 RNA polymerase. Biochemistry 1988, 27, 3966-3974.
12. Milligan, J. F.; Uhlenbeck, O. C. Synthesis of small RNAs using T7 RNA polymerase. Methods Enzymol. 1989, 180, 51-62.
13. Kern, J. A.; Davis, R. H. Application of solution equilibrium analysis to in vitro RNA transcription. Biotechnol. Prog. 1997, 13, 747-756.
14. Cunningham, P. R.; Ofengard, J. The use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 polymerase. Biotechniques 1990, 9, 713-714.
15. Walaas, E. Stability constants of metal complexes with mononucleotides. Acta Chem. Scand. 1968, 12, 528-536.
16. Young, J. S.; Ramirez, W. F.; Davis, R. H. Modeling and optimization of a batch process for in vitro RNA production. Biotechnol. Bioeng. 1997, 56, 210-220.
17. Ling, M. L.; Risman, S. S.; Klement, J. F.; McGraw, N.; McAllister, W. T. Abortive initiation by bacteriophage T3 and T7 RNA polymerases under conditions of limiting substrate. Nucleic Acids Res. 1989, 17, 1605-1618.
18. Davanloo, P.; Rosenberg, A. H.; Dunn, J. J.; Studier, F. W. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. U.S.A. 1984, 81, 2035-2039.