Calcitonin gene-related peptide (CGRP) increases intracellular free Ca2+ concentrations but not cyclic AMP formation in CGRP receptor-positive osteosarcoma cells (OHS-4)

Cytokine

Volumn 11, Issue 3, 1999, Pages 200-207

  Drissi, H ^a   Lieberherr, M ^b   Hott, M ^a   Marie, P J ^a   Lasmoles, F ^a  

^a HÔPITAL LARIBOISIÈRE   (France)

^b CNRS   (France)

Author keywords

Calcitonin gene related peptide; Calcium; Osteoblast; Signal transduction

Indexed keywords

ADENYLATE CYCLASE; CALCITONIN GENE RELATED PEPTIDE; CALCITONIN GENE RELATED PEPTIDE RECEPTOR; CALCIUM ION; CYCLIC AMP; PARATHYROID HORMONE; PHOSPHOLIPASE C;

ARTICLE; CALCIUM CELL LEVEL; CELL CYCLE; CELL CYCLE G0 PHASE; CELL CYCLE S PHASE; CELL LEVEL; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; HUMAN; HUMAN CELL; OSTEOBLAST; OSTEOSARCOMA CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION;

EID: 0033103385     PISSN: 10434666     EISSN: None     Source Type: Journal    
DOI: 10.1006/cyto.1998.0415     Document Type: Article

References (47)

1. Amara SG, Jones V, Rosenfeld MG, Ong ES, Evans RM (1982) Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptides products. Nature 29:240-249.
2. Rosenfeld MG, Mermod JJ, Amara SG, Swanson LW, Sawachenko DE, Rivier J, Vale WE (1983) Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing. Nature 304:120-135.
3. Ahmed M, Srinivasan GR, Theodorsson E, Bjurholm A, Kreicbergs A (1994) Extraction and quantitation of neuropeptides in bone by radioimmunoassay. Regul Pept 51:179-188.
4. Bjurholm A, Kreicbergs A, Brodin E, Schultzberg M (1988) Substance P- and CGRP-immunoreactive nerves in bone. Peptide 9:165-171.
5. Aoki M, Tamai K, Saotome K (1994) Substance P- and calcitonin gene-related peptide-immunofluorescent nerves in the repair of experimental bone defects. Intern Orthopaed 18:317-324.
6. Hara-Irie F, Amizuka N, Ozawa H (1996) Immunohisto-chemical and ultrastructural localization of CGRP-ipositive nerve fibers at the epiphyseal trabecules facing the growth plate of rat femurs. Bone 18:29-39.
7. Burns DM, Hill EL, Howard GA, Roos BA (1991) Are osteoblasts the skeletal target for the actions of calcitonin gene-related peptide? (Abstract). J Bone Miner Res 6:S285.
8. Hill EL, Elde R (1988) Calcitonin gene-related peptide-immunoreactive nerve fibers in mandibular periosteum of rat: Evidence for primary afferent origin. Neurosci Lett 85:172-178.
9. Hukkanen M, Konttinen YT, Santavirta S, Paavolainen P, Gu XH, Terenghi G, Polak JM (1993) Rapid proliferation of calcitonin gene-related peptide-immunoreactive nerves during healing of rat tibial fracture suggests neural involvement in bone growth and remodeling. Neuroscience 54:969-979.
10. Bernard GW, Shih C (1990) The osteogenic stimulating effect of neuroactive calcitonin gene-related peptide. Peptides 11:625-632.
11. Imai S, Rauvala H, Konttinen YT, Tokunaga T, Maeda T, Hukuda S, Santavirta S (1997) Efferent targets of osseous CGRP-immuno-reactive nerve fiber before and after bone destruction in adjuvant arthritic rat: An ultramorphological study on their terminal-target relations. J Bone Miner Res 12:1018-1027.
12. Shih C, Bernard GW (1997) Calcitonin gene related peptide enhances bone colony development in vitro. Clin Orthop Relat Res 334:335-344.
13. Aiyar N, Rand K, Elshourbagy NA, Zeng Z, Adamou JE, Bergsma DJ, Li Y (1996) A cDna encoding the calcitonin gene-related peptide type 1 receptor. J Biol Chem 271:11325-11329.
14. Crawford A, Evans B, Skjodt H, Beresford JN, MacIntyre I, Russell RGG (1985) Effect of human calcitonin gene related peptide on human bone-derived cells in culture (Abstract). Bone 7:156-157.
15. Millet I, Juluru K, McCarthy TL, Centrella M, Casinghino S, Vignery A (1995) Calcitonin gene-related peptide differentially regulates cytokines (TNF-a, IL-6) and insulin-like growth factor-1 production by primary osteoblasts (Abstract). J Bone Miner Res 10:S272.
16. Vignery A, MacCarthy TL (1996) The neuropeptide calcitonin gene-related peptide stimulates insulin-like growth factor 1 production by primary fetal rat osteoblasts. Bone 18:331-335.
17. Michelangeli VP, Fletcher A, Allan EH, Nicholson GC, Martin TJ (1989) Effect of Calcitonin gene-related peptide on cyclic AMP formation in chicken, rat and mouse bone cells. J Bone Miner Res 4:269-272.
18. Sakagami Y, Girasole G, Yu XP, Boswell HS, Manolagas SC (1993) Stimulation of interleukin-6 production by either calcitonin gene-related peptide or parathyroid hormone in two phenotypically distinct marrow-derived murine stroma cells. J Bone Miner Res 8:811-816.
19. Bjurholm A (1991) Neuroendocrine peptides in bone. Intern Orthopaed 15: 325-329.
20. Demarest KT, Xu JZ, Minor LK, Gunnet J, Cryan E (1996) Calcitonin gene related peptide (CGRP) receptor in human SAOS-2 and TE-85 osteosarcoma cells (Abstract). J Bone Miner Res 11:S372.
21. 45Ca uptake and alters alkaline phosphatase specific activity in human osteosarcoma cells. Metabolism 42:97-104.
22. Van Valen F, Keck E, Jürgens H (1989) Functional characteristics of calcitonin gene related peptide receptors in human Ewing's sarcoma WE-68 cells. FEBS Lett 256:170-174.
23. Bjurholm A, Kreicbergs A, Schultzberg M, Lerner UH (1992) Neuroendocrine regulation of cyclic AMP formation in osteoblastic cell lines (UMR10601, ROS17/28, MC3T3-E1 and Saos-2) and primary bone cells. J Bone Miner Res 7:1011-1019.
24. Michelangeli VP, Findlay DM, Fletcher A, Martin TJ (1986) Calcitonin gene-related peptide (CGRP) acts independently of calcitonin on cyclic AMP formation in clonal osteogenic sarcoma cells (UMR 106-01). Calcif Tissue Int 39:44-48.
25. Zaidi M, Datta HK, Chambers TJ, MacIntyre I (1989) Production and characterisation of immunoreactive calcitonin gene-related peptide (CGRP) from a CGRP receptor-positive cloned osteosarcoma cell line (UMR 106-01). Biochem Biophys Res Commun 168:214-219.
26. Thiebaud D, Akatsu T, Yamashita T, Suda T, Noda T, Martin RE, Fletcher AE, Martin TJ (1991) Structure-activity relationships in calcitonin gene-related peptide: Cyclic AMP response in a preosteoblast cell line (KS-4). J Bone Miner Res 6:1137-1142.
27. 2+ concentrations in UMR106 osteoblastic cells. Bone 16:379S-384S.
28. Drissi H, Hott M, Marie PJ, Lasmoles F (1997) Expression of CT/CGRP gene and its regulation by dibutyryl cyclic adenosine monophosphate in human osteoblastic cells. J Bone Miner Res 12:1805-1814.
29. Fournier B, Price PA (1991) Characterization of a new human osteosarcoma cell line OHS-4. J Cell Biol 114:577-583.
30. Marie PJ, Lomri A, Sabbagh A, Basle M (1989) Culture and behavior of osteoblastic cells isolated from normal trabecular bone surfaces. In Vitro Cell Biol Dev 25:373-380.
31. 3. Kidney Int 31:736-743.
32. Chakraborty M, Chatterjee D, Kellokumpu S, Rasmussen H, Baron R (1991) Cell cycle-dependent coupling of the calcitonin receptor to different G proteins. Science 51:1078-1082.
33. Abou-Samra A, Jüppner H, Force T, Freeman MW, Kong X, Schipani E, Urena P, Richards J, Bonventre JV, Potts Jr JT, Kronenberg HM, Segre G (1992) Expression cloning of a common receptor for parathyroid hormone and parathyroid hormone-related peptide from rat osteoblast-like cells: A single receptor stimulates intracellular accumulation of both cAmp and inositol triphosphates and increases intracellular free calcium. Proc Natl Acad Sci USA 89:2732-2736.
34. Force T, Bonventre JV, Flannery MR, Gorn AH, Yamin M, Goldring SR (1992) A cloned porcine renal calcitonin receptor coupled to adenylyl cyclase and phospholipase C. Am J Physiol 262:F1110-1115.
35. Spengler D, Waeber C, Pantaloni C, Holsboer F, Bockaert J, Seeburg PH, Journot L (1993) Differential signal transduction by five splice variants of the PACAP receptor. Nature 365:170-175.
36. Wheeler MB, Lu M, Dillon JS, Leng XH, Chen C, Boyd AE (1993) Functional expression of the rat glucagon-like peptide-1 receptor: Evidence for coupling to both adenylyl cyclase and phospholipase-C. Endocrinology 133:57-62.
37. Aiyar N, Baker E, Nambi P, Feuerstein G, Willette R (1994) Characterization of CGRP receptors in various regions of gerbil brain. Neuropeptides 26:313-317.
38. Goltzmann D, Mitchell J (1985) Interaction of calcitonin and calcitonin gene-related peptide at receptor sites in target tissues. Science 227:1343-1345.
39. Stangl D, Muff R, Schmolck C, Fischer JA (1993) Photo-affinity labeling of rat calcitonin gene related peptide receptors and adenylate cyclase activation: Identification of receptor subtypes. Endocrinology 132:744-750.
40. Flühmann B, Muff R, Hunziker W, Fisher JA, Born W (1995) A human orphan calcitonin receptor-like structure. Biochem Biophys Res Commun 206:341-347.
41. Han ZQ, Coppock HA, Smith DM, VanNoorden S, Makgoba MW, Nicholl CG, Legon S (1997) The interaction of CGRP and adrenomedullin with a receptor expressed in the rat pulmonary vascular endothelium. J Mol Endocr 18:267-272.
42. Njuki F, Nicholl CG, Howard A, Mak JC, Barnes PJ, Girgis SI, Legon S (1993) A new calcitonin-receptor-like sequence in rat pulmonary blood vessels. Clin Science 85:385-388.
43. Dennis T, Fournier A, Cadieux A, Pomerleau F, Jolicoeur FB, St Pierre S, Quirion R (1990) hCgrp(8-37), a CGRP antagonist revealing calcitonin gene related peptide receptor heterogeneity in brain and periphery. J Pharmacol Exp Therapeutics 254:123-128.
44. Chomczynski P, Sacchi, N (1987) Single step method of RNA isolation by acid guanidium thyocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159.
45. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265-275.
46. 3 metabolites on cytosolic free calcium in confluent mouse osteoblasts. J Biol Chem 262:13168-13173.
47. 2+ indicators with greatly improved fluorescent properties. J Biol Chem 260:3440-3450.