pFab-CMV, a single vector system for the rapid conversion of recombinant Fabs into whole IgG1 antibodies

Immunotechnology

Volumn 4, Issue 3-4, 1999, Pages 185-188

  Sanna, Pietro Paolo ^a   Samson, Maria E ^a   Moon, Jenny S ^a   Rozenshteyn, Roman ^a   De Logu, Alessandro ^a,b   Williamson, R Anthony ^a   Burton, Dennis R ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF CAGLIARI   (Italy)

Author keywords

Antibody engineering; Eukaryotic cells; Expression vector; Human monoclonal antibodies; Immunoglobulin expression; Phage antibody libraries

Indexed keywords

CYTOMEGALOVIRUS ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; RECOMBINANT ANTIBODY; VIRUS VECTOR;

ANTIBODY PRODUCTION; ARTICLE; CYTOMEGALOVIRUS; GENETIC TRANSFECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BACTERIOPHAGES; CHO CELLS; CRICETINAE; CYTOMEGALOVIRUS; GENETIC VECTORS; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN G; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 0033103202     PISSN: 13802933     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1380-2933(98)00022-0     Document Type: Article

References (13)

1. Barbas C.F., Kang A.S., Lerner R.A., Benkovic S.J. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc Natl Acad Sci USA. 88(18):1991;7978-7982.
2. Burioni R., Williamson R.A., Sanna P.P., Bloom F.E., Burton D.R. Recombinant human Fab to glycoprotein D neutralizes infectivity and prevents cell-to-cell transmission of herpes simplex viruses 1 and 2 in vitro. Proc Natl Acad Sci USA. 91(1):1994;355-359.
3. Chanock R.M., Crowe J.J., Murphy B.R., Burton D.R. Human monoclonal antibody Fab fragments cloned from combinatorial libraries: potential usefulness in prevention and/or treatment of major human viral diseases. Infect Agents Dis. 2(3):1993;118-131.
4. Sanna P.P., Williamson R.A., De Logu A., Bloom F.E., Burton D.R. Directed selection of recombinant human monoclonal antibodies to herpes simplex glycoproteins from phage display libraries. Proc Natl Acad Sci USA. 92(13):1995;6439-6443.
5. Williamson R.A., Burioni R., Sanna P.P., Partridge L.J., Barbas C.D., Burton D.R. Human monoclonal antibodies against a plethora of viral pathogens from single combinatorial libraries. Proc Natl Acad Sci USA. 90(9):1993;4141-4145.
6. Winter G., Griffiths A.D., Hawkins R.E., Hoogenboom H.R. Making antibodies by phage display technology. Annu Rev Immunol. 12(433):1994;433-455.
7. Lerner R.A., Kang A.S., Bain J.D., Burton D.R., Barbas C.D. Antibodies without immunization. Science. 258(5086):1992;1313-1314.
8. Bender E., Woof J.M., Atkin J.D., Barker M.D., Bebbington C.R., Burton D.R. Recombinant human antibodies: linkage of an Fab fragment from a combinatorial library to an Fc fragment for expression in mammalian cell culture. Hum Antibodies Hybridomas. 4(2):1993;74-79.
9. Ames R.S., Tornetta M.A., Deen K., Jones C.S., Swift A.M., Ganguly S. Conversion of murine Fabs isolated from a combinatorial phage display library to full length immunoglobulins. J Immunol Methods. 184(2):1995;177-186.
10. Norderhaug L., Olafsen T., Michaelsen T.E., Sandlie I. Versatile vectors for transient and stable expression of recombinant antibody molecules in mammalian cells. J Immunol Methods. 204(1):1997;77-87.
11. Persic L., Roberts A., Wilton J., Cattaneo A., Bradbury A., Hoogenboom H.R. An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries. Gene. 187(1):1997;9-18.
12. Sanna P.P., De L.A., Williamson R.A., Hom Y.L., Straus S.E., Bloom F.E., Burton D.R. Protection of nude mice by passive immunization with a type-common human recombinant monoclonal antibody against HSV. Virology. 215(1):1996;101-106.
13. Takahashi N., Ueda S., Obata M., Nikaido T., Nakai S., Honjo T. Structure of human immunoglobulin gamma genes: implications for evolution of a gene family. Cell. 29(2):1982;671-679.