Measurement of bile salt hydrolase activity from Lactobacillus acidophilus based on disappearance of conjugated bile salts

Journal of Dairy Science

Volumn 82, Issue 3, 1999, Pages 466-471

  Corzo, G ^a,b   Gilliland, S E ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

^b SUNTORY INSTITUTE FOR BIOORGANIC RESEARCH   (Japan)

Author keywords

Bile salt hydrolase; Lactobacillus acidophilus; Sodium glycocholate; Sodium taurocholate

Indexed keywords

LACTOBACILLUS ACIDOPHILUS;

AMIDASE; BILE ACID; CHOLOYLGLYCINE HYDROLASE; EDETIC ACID; GLYCOCHOLIC ACID; TAUROCHOLIC ACID;

ARTICLE; ENZYME STABILITY; ENZYMOLOGY; FREEZING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; LACTOBACILLUS ACIDOPHILUS; METABOLISM; PH; PRECIPITATION;

AMIDOHYDROLASES; BILE ACIDS AND SALTS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; EDETIC ACID; ENZYME STABILITY; FREEZING; GLYCOCHOLIC ACID; HUMANS; HYDROGEN-ION CONCENTRATION; LACTOBACILLUS ACIDOPHILUS; PRECIPITATION; TAUROCHOLIC ACID;

EID: 0033088387     PISSN: 00220302     EISSN: None     Source Type: Journal    
DOI: 10.3168/jds.S0022-0302(99)75255-0     Document Type: Article

References (22)

1. Aries, V., and M. J. Hill. 1970. Degradation of steroids by intestinal bacteria. I. Deconjugation of bile salts. Biochim. Biophys. Acta 202:526-534.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Anal. Biochem. 72:248-254.
3. Dashkevics, M. P., and S. D. Feighner. 1989. Development of a differential medium for bile salt hydrolase-active Lactobacillus spp. Appl. Environ. Microbiol. 55:11-16.
4. Dekker, R., R. Van der Meer, and C. Olieman. 1991. Sensitive pulsed amperometric detection of free and conjugated bile acids in combination with gradient reversed-phase HPLC. Chromatographia 31:549-553.
5. De Rodas, B. Z., S. E. Gilliland, and C. V. Maxwell. 1996. Hypocholesterolemic action of Lactobacillus acidophilus ATCC 43121 and calcium in swine with hypercholesterolemia induced by diet. J. Dairy Sci. 79:2121-2128.
6. De Smet, I., L. Van Hoorde, M. De Saeyer, M. Vande Woestyne, and W. Verstraete. 1994. In Vitro study of bile salt hydrolase (BSH) activity of BSH isogenic Lactobacillus plantarum 80 strains and estimation of cholesterol lowering through enhanced BSH activity. Microb. Ecol. Health Dis. 7:315-329.
7. Feighner, S. D., and M. P. Dashkevics. 1988. Effects of dietary carbohydrates on bacterial cholyltaurine hydrolase in poultry intestinal homogenates. Appl. Environ. Microbiol. 54:337-342.
8. Gilliland, S. E. 1990. Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol. Rev. 87:175-188.
9. Gilliland, S. E., C. R. Nelson, and C. Maxwell. 1985. Assimilation of cholesterol by Lactobacillus acidophilus. Appl. Environ. Microbiol. 49:377-381.
10. Gilliland, S. E., and M. L. Speck. 1977. Deconjugation of bile acids by intestinal lactobacilli. Appl. Environ. Microbiol. 33: 15-18.
11. Gopal-Srivastava, R., and P. B. Hylemon. 1988. Purification and characterization of bile salt hydrolase from Clostridium perfringens. J. Lipid Res. 29:1079-1085.
12. Grill, J. P., F. Schneider, J. Crociani, and J. Ballongue. 1995. Purification and characterization of conjugated bile salt hydrolase from Bifidobacterium longum BB536. Appl. Environ. Microbiol. 61:2577-2582.
13. Hammes, W. P., N. Weiss, N., and W. Holzapfel. 1992. The Genera Lactobacillus and Carnobacterium. Pages 1535-1594 in The Prokaryotes. Vol. II. A. Balows, H. Trüper, M. Dworkin, W. Harder, and K. H. Schleifer, ed. Springer-Verlag, New York.
14. Iida, T., Y. Ohnuki, F. C. Chang, J. Goto, and T. Nambara. 1985. High performance liquid chromatographic separation of stereoisomeric bile acids as their uv-sensitive esters. Lipids 20: 187-194.
15. Kawamoto, K., I. Horibe, and K. Uchida. 1989. Purification and characterization of a new hydrolase for conjugated bile acids, chenodeoxycholyltaurine hydrolase, from Bacteroides vulgatus. J. Biochem. 106:1049-1053.
16. Kirby, L. C., R. A. Klein, and J. P. Coleman. 1995. Continuous spectrophotometric assay of conjugated bile acid hydrolase. Lipids 30:863-867.
17. Lundeen, S., and D. C. Savage. 1990. Characterization and purification of bile salt hydrolase from Lactobacillus sp. strain 100-100. J. Bacteriol. 172:4171-4177.
18. Ruben, A. T., and G. P. Van Berge-Henegouwen. 1982. A simple reverse-phase high pressure liquid chromatographic determination of conjugated bile acids in serum and bile using a novel radial compressions separation system. Clin. Chim. Acta 119: 41-50.
19. SAS® Procedures Guide for Personal Computers. 1985. SAS Inst., Inc., Cary, NC.
20. Scalia, S. 1987. Evaluation of mobile and stationary phases in reversed-phase high-performance liquid chromatography of conjugated bile acids. J. Liquid Chromatogr. 10:2055-2080.
21. Stellwag, E. J., and P. B. Hylemon. 1976. Purification and characterization of bile salt hydrolase from Bacteroides fragilis subsp. fragilis. Biochim. Biophys. Acta 452:165-176.
22. Walker, D. K., and S. E. Gilliland. 1993. Relationships among bile tolerance, bile salt deconjugation, and assimilation of cholesterol by Lactobacillus acidophilus. J. Dairy Sci. 76: 956-961.