Expression, purification, and characterization of recombinant forms of membrane-bound cytochrome c-550nm from Bacillus subtilis

Protein Expression and Purification

Volumn 15, Issue 1, 1999, Pages 69-76

  David, Pamela S ^a   Morrison, Megan R ^a   Wong, Sui Lam ^b   Hill, Bruce C ^a  

^a QUEEN S UNIVERSITY   (Canada)

^b UNIVERSITY OF CALGARY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; CYTOCHROME C; EDETIC ACID; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; Q SEPHAROSE;

BACILLUS SUBTILIS;

EID: 0033083509     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.1001     Document Type: Article

References (32)

1. von Wachenfeldt C., Hederstedt L. Bacillus subtiliscccc. J. Biol. Chem. 265:1990;13939-13948.
2. Pettigrew G. W., Moore G. R. Cytochromesc. 1987;Springer-Verlag, Berlin.
3. Bushnell G. W., Louie G. V., Brayer G. D. High-resolution three-dimensional structure of horse heart cytochromec. J. Mol. Biol. 214:1990;585-595.
4. 1. Biochim. Biophys. Acta. 592:1980;519-527.
5. 1. J. Biol. Chem. 255:1980;4732-4739.
6. von Wachenfeldt C., Hederstedt L. Physico-chemical characterization of membrane-bound and water-soluble forms ofBacillus subtilisc. Eur. J. Biochem. 212:1993;499-509.
7. Yu J., Hederstedt L., Piggot P. J. The cytochromebccBacillus subtilis. J. Bacteriol. 177:1995;6751-6760.
8. Saraste M., Nakari T., Metso T., Jalli T., Lauraeus M., van der Oost J. TheBacillus subtilisc. Eur. J. Biochem. 195:1991;517-525.
9. Huang X. On global sequence alignment. Comput. Appl. Biosci. 10:1994;227-235.
10. Lemma E., Schagger H., Kroger A. The menaquinol oxidase ofBacillus subtilis. Arch. Microbiol. 159:1993;574-578.
11. von Wachenfeldt C., Hederstedt L. Bacillus subtiliscEscherichia coli. FEBS Lett. 270:1990;147-151.
12. Higuchi R. Using PCR to engineer DNA. PCR Technology Principles and Applications for DNA Amplification. 1989;Stockton Press, New York. p. 61-70.
13. Erlich H. A., Gelfand D., Sninsky J. J. Recent advances in the polymerase chain reaction. Science. 252:1991;1643-1651.
14. Haima P., Bron S., Venema G. The effect of restriction on shotgun cloning and plasmid stability inBacillus subtilis. Mol. Gen. Genet. 209:1987;335-342.
15. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1987;Cold Spring Harbor Laboratory Press, Plainview.
16. Gryczan T. J., Contente S., Dubnau D. Characterization ofStaphylococcus aureusBacillus subtilis. J. Bacteriol. 134:1978;318-329.
17. Dubnau D., Davidoff-Abelson R. Fate of transforming DNA following uptake by competentBacillus subtilis. J. Mol. Biol. 56:1971;209-221.
18. Bron S. Plasmids. Harwood C. R., Cutting S. M. Molecular Biological Methods forBacillus. 1990;75-139 Wiley, Chichester.
19. Kawamura F., Doi R. H. Construction ofBacillus subtilis. J. Bacteriol. 160:1984;442-444.
20. DeVrij W., Azzi A., Konings W. N. Structural and functional properties of cytochromecBacillus subtilis. Eur. J. Biochem. 131:1983;97-103.
21. 3Bacillus subtilis. Biochem. J. 309:1995;279-283.
22. Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provenzo M. D., Fujimoto E. K., Goeke N. M., Olsen B. J., Klenk D. C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:1985;76-85.
23. MacDonnel A., Staehelin L. A. Detection of cytochromef. Anal. Biochem. 40:1981;177-180.
24. Dutton P. L. Redox potentiometry: Determination of midpoint potentials of oxidation-reduction components of biological electron-transfer systems. Methods Enzymol. LIV:1978;411-435.
25. 6Bacillus subtilis. Gene. 147:1994;91-94.
26. McDonald C. C., Phillips W. D., Vinogradov S. S. Proton magnetic resonance evidence for methionine-iron coordination in mammalian-type ferrocytochromec. Biochem. Biophys. Res. Commun. 36:1969;442-449.
27. Harbury H. A., Cronin J. R., Fanger M. W., Hettinger T. P., Murphy A. J., Myer Y. P., Vinogradov S. N. Complex formation between methionine and a heme peptide from cytochromec. Proc. Natl. Acad. Sci. USA. 54:1965;1658-1666.
28. DeVrij W., van den Burg B., Konings W. N. Spectral and potentiometric analysis of cytochromes fromBacillus subtilis. Eur. J. Biochem. 166:1987;589-595.
29. Hagen W. R. Direct electron transfer of redox proteins at the bare glassy carbon electrode. Eur. J. Biochem. 182:1989;523-530.
30. Ivanetich K. M., Bradshaw J. J., Kaminsky L. S. Methionine sulfoxide cytochromec. Biochemistry. 15:1976;1144-1153.
31. Keilin D. Cytochrome and intracellular oxidase. Proc. R. Soc. London B. 106:1930;418-444.
32. Stellwagen E. Haem exposure as the determinate of oxidation-reduction potential of haem proteins. Nature. 275:1978;73-74.