Core RNA polymerase from E. coli induces a major change in the domain arrangement of the σ70 subunit

Molecular Cell

Volumn 3, Issue 2, 1999, Pages 229-238

  Callaci, Sandhya ^a   Heyduk, Ewa ^a   Heyduk, Tomasz ^a   Doisy, Edward A ^a  

^a SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

ARTICLE; BINDING AFFINITY; CONFORMATIONAL TRANSITION; DNA BINDING; ENERGY TRANSFER; ENZYME ACTIVITY; ENZYME BINDING; ENZYME SUBUNIT; ESCHERICHIA COLI; LUMINESCENCE; MOLECULAR MODEL; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN TERTIARY STRUCTURE;

ESCHERICHIA COLI;

EID: 0033083036     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(00)80313-5     Document Type: Article

References (34)

1. Burgess, R.R., and Jendrisak, J.J. (1975). A procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatography. Biochemistry 14, 4634-4638.
2. Burgess, R.R., and Travers, A.A. (1970). Escherichia coli RNA polymerase: Purification, subunit structure, and factor requirements. Fed. Proc. 29, 1164-1169.
3. Burgess, R.R., Travers, A.A., Dunn, J.J., and Bautz, E.K. (1969). Factor stimulating transcription by RNA polymerase. Nature 221, 43-46.
4. Callaci, S., and Heyduk, T. (1998). Conformation and DNA binding properties of a single-stranded DNA binding region of sigma 70 subunit from Escherichia coli RNA polymerase are modulated by an interaction with the core enzyme. Biochemistry 37, 3312-3320.
5. 70 factor induced by binding to the core enzyme. J. Biol. Chem. 273, 32995-33001.
6. Carson, M. (1991). Ribbons 2.0. J. Appl. Crystallogr. 24, 958-961.
7. Chang, J.Y., Knecht, R., and Braun, D.G. (1983). A new method for the selective isolation of cysteine-containing peptides. Specific labelling of the thiol group with a hydrophobic chromophore. Biochem. J. 211, 163-171.
8. Daughdrill, G.W., Hanely, L.J., and Dahlquist, F.W. (1998). The C-terminal half of the anti-sigma factor FlgM contains a dynamic equilibrium solution structure favoring helical conformations. Biochemistry 37, 1076-1082.
9. Dombroski, A.J., Walter, W.A., Record, M.T., Jr, Siegele, D.A., and Gross, C.A. (1992). Polypeptides containing highly conserved regions of transcription initiation factor sigma 70 exhibit specificity of binding to promoter DNA. Cell 70, 501-512.
10. Dombroski, A.J., Walter, W.A., and Gross, C.A. (1993a). Amino-terminal amino acids modulate sigma-factor DNA-binding activity. Genes Dev. 7, 2446-2455.
11. Dombroski, A.J., Walter, W.A., and Gross, C.A. (1993b). The role of the sigma subunit in promoter recognition by RNA polymerase. Cell. Mol. Biol. Res. 39, 311-317.
12. Dombroski, A.J., Johnson, B.D., Lonetto, M., and Gross, C.A. (1996). The sigma subunit of Escherichia coli RNA polymerase senses promoter spacing. Proc. Natl. Acad. Sci. USA 93, 8858-8862.
13. Ebright, R.H., and Busby, S. (1995). The Escherichia coli RNA polymerase alpha subunit: Structure and function. Curr. Opin. Genet. Dev. 5, 197-203.
14. Ferrin, T.E., Huang, C.C., Jarvis, L.E., and Langridge, R. (1988). The MIDAS display system. J. Mol. Graph. 6, 13-27.
15. Förster, T. (1948). Zwischenmolekulare energiewanderung und fluoreszenz. Ann. Phys. 2, 55-75.
16. Gernstein, M., and Krebs, W. (1998). A database of macromolecular motions. Nucleic Acids Res. 26, 4280-4290.
17. Gribskov, M., and Burgess, R.R. (1983). Overexpression and purification of the sigma subunit of Escherichia coli RNA polymerase. Gene 26, 109-118.
18. Heyduk, E., and Heyduk, T. (1997).Thiol-reactive, luminescent europium chelates: Luminescence probes for resonance energy transfer distance measurements in biomolecules. Anal. Biochem. 248, 216-227.
19. 70 subunit of E. coli RNA polymerase and the nontemplate strand oligonucleotide: Luminescence resonance energy transfer study. J. Biol. Chem. 274, 3315-3322.
20. Hinkle, D.C., and Chamberlin, M.J. (1972). Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection. J. Mol. Biol. 70, 157-185.
21. Lonetto, M., Gribskov, M., and Gross, C.A. (1992). The sigma 70 family: Sequence conservation and evolutionary relationships. J. Bacteriol. 174, 3843-3849.
22. Lowe, P.A., Hager, D.A., and Burgess, R.R. (1979). Purification and properties of the sigma subunit of Escherichia coli DNA-dependent RNA polymerase. Biochemistry 18, 1344-1352.
23. Malhotra, A., Severinova, E., and Darst, S.A. (1996). Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase. Cell 87, 127-136.
24. Mujumdar, R.B., Ernst, L.A., Mujumdar, S.R., Lewis, C.J., and Waggoner, A.S. (1993). Cyanine dye labeling reagents: Sulfoindocyanine succinimidyl esters. Bioconj. Chem. 4, 105-111.
25. 70), promoters, and the kinetics of the steps of transcription initiation. In Escherichia Coli and Salmonella: Cellular and Molecular Biology, 2nd Ed., F.C. Neidhardt, R. Curtis III, J.L. Ingraham, E.C.C. Lin, K.R. Low, B. Magasanik, W.S. Reznikoff, M. Riley, M. Schaechter, and H.E. Umbarger, eds. (Washington, DC: ASM Press), 792-820.
26. Sanger, F., Nicklen, S., and Coulson, A.R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
27. Selvin, P.R. (1995). Fluorescence resonance energy transfer. Methods Enzymol. 246, 300-334.
28. Selvin, P.R. (1996). Lanthanide-based resonance energy transfer. IEEE J. Select Top. Quant. Electron. 2, 1077-1087.
29. Selvin, P.R., and Hearst, J.E. (1994). Luminescence energy transfer using a terbium chelate: Improvements on fluorescence energy transfer. Proc. Natl. Acad. Sci. USA 91, 10024-10028.
30. Selvin, P.R., Rana, T.M., and Hearst, J.E. (1994). Luminescence energy transfer. J. Am. Chem. Soc. 116, 6029-6030.
31. Sippel, T.O. (1981). New fluorochromes for thiols: Maleimide and iodoacetamide derivatives of a 3-phenylcoumarin fluorophore. J. Histochem. Cytochem. 29, 314-316.
32. Straney, D.C., and Crothers, D.M. (1985). Intermediates in transcription initiation from the E. coli lac uv5 promoter. Cell 43, 449-459.
33. Wellman, A., and Meares, C.F. (1991). Footprint of the sigma protein: A re-examination. Biochem. Biophys. Res. Comm. 177, 140-144.
34. Wu, F.Y., Yarbrough, L.R., and Wu, C.W. (1976). Conformational transition of Escherichia coli RNA polymerase induced by the interaction of sigma subunit with core enzyme. Biochemistry 15, 3254-3258.