Site-directed mutagenesis of serine 158 demonstrates its role in spinach leaf sucrose-phosphate synthase modulation

Plant Journal

Volumn 17, Issue 4, 1999, Pages 407-413

  Toroser, Dikran ^a   McMichael Jr , Robert ^a,f   Krause, Klause Peter ^b   Kurreck, Jens ^c   Sonnewald, Uwe ^c   Stitt, Mark ^d   Huber, Steven C ^a,e  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b MOGEN International NV   (Netherlands)

^c Inst Plant Genet Crop Plant Res   (Germany)

^d UNIVERSITY OF HEIDELBERG   (Germany)

^e NORTH CAROLINA STATE UNIVERSITY   (United States)

^f UTS Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME MODIFICATION; ENZYME PURIFICATION; MONOCLONAL ANTIBODY; PROTEIN PHOSPHORYLATION; SPINACH; SUCROSE PHOSPHATE SYNTHASE KINASE; SUCROSE PHOSPHATE SYNTHASE; TARGETED MUTAGENESIS; TOBACCO; TRANSGENE; TRANSGENIC PLANT;

NASA DISCIPLINE PLANT BIOLOGY; NON-NASA CENTER;

BASE SEQUENCE; CIRCADIAN RHYTHM; DNA PRIMERS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GLUCOSYLTRANSFERASES; MUTAGENESIS, SITE-DIRECTED; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; SERINE; SPINACIA OLERACEA; TOBACCO;

EMBRYOPHYTA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; SPINACIA OLERACEA;

EID: 0033082670     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.1999.00389.x     Document Type: Article

References (27)

1. Bachmann, M., Huber, J.L., Athwal, G.S., Wu, K., Ferl, R.J. and Huber, S.C. (1996a) 14-3-3 proteins associate with the regulatory phosphorylation site of spinach leaf nitrate reductase in an isoform-specific manner and reduce dephosphorylation of Ser-543 by endogenous protein phosphatases. FEBS Lett. 398, 26-30.
2. Bachmann, M., Huber, J.L., Liao, P.-C., Gage, D.A. and Huber, S.C. (1996b) The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein. FEBS Lett. 387, 127-131.
3. Barber, G.A. (1985) The equilibrium of the reaction catalyzed by sucrose phosphate synthase. Plant Physiol. 79, 1127-1128.
4. Doehlert, D.C. and Huber, S.C. (1983) Regulation of spinach leaf sucrose-phosphate synthase by glucose-6-phosphate, inorganic phosphate, and pH. Plant Physiol. 73, 989-994.
5. Hoefgen, R. and Willmitzer, L. (1990) Biochemical and genetic analysis of different patatin isoforms expressed in various organs of potato (Solanum tuberosum L). Plant Sci. 66, 221-230.
6. Huber, J.L.A. and Huber, S.C. (1992a) Site-specific serine phosphorylation of spinach leaf sucrose-phosphate synthase. Biochem. J. 283, 877-882.
7. Huber, J.L.A. and Huber, S.C. (1996) Role and regulation of sucrose-phosphate synthase in higher plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, 431-444.
8. Huber, J.L.A., Huber, S.C. and Nielsen, T.H. (1989a) Protein phosphorylation as a mechanism for regulation of spinach leaf sucrose-phosphate synthase activity. Arch. Biochem. Biophys. 270, 681-690.
9. Huber, S.C. and Huber, J.L. (1992b) Role of sucrose-phosphate synthase in sucrose metabolism in leaves. Plant Physiol. 99, 1275-1278.
10. Huber, S.C., Huber, J.L.A. and McMichael, R.W. Jr (1993) The regulation of sucrose synthesis in leaves. In Carbon Partitioning Within and Between Organisms (Pollock, C.J., Farrar, J.F. and Gordon, A.J., eds). Oxford: Oxford University Press, pp. 1-26.
11. 2+]. UC-Riverside Symposium Proceedings. In Carbon Partitioning and Source - Sink Interactions in Plants (Madore, M.A. and Lucas, W.J., eds). Rockville: American Society of Plant Physiologists, pp. 35-44.
12. Huber, S.C., Nielsen, T.H., Huber, J.L.A. and Pharr, D.M. (1989b) Variation among species in light activation of sucrose-phosphate synthase. Plant Cell Physiol. 30, 277-286.
13. Kurland, I.J., el-Maghrabi, M.R., Correia, J.J. and Pilkis, S.J. (1992) Rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Properties of phospho- and dephospho-forms and of two mutants in which Ser32 has been changed by site-directed mutagenesis. J. Biol. Chem. 267, 4416-4423.
14. Lunn, J.E. and ap Rees, T. (1990) Purification and properties of sucrose-phosphate synthase from seeds of Pisum sativum. Phytochem. 29, 1057-1064.
15. McMichael, R.W. Jr, Bachmann, M. and Huber, S.C. (1995) Spinach leaf sucrose-phosphate synthase and nitrate reductase are phosphorylated/inactivated by multiple protein kinases in vitro. Plant Physiol. 108, 1077-1082.
16. McMichael, R.W. Jr, Klein, R.R., Salvucci, M.E. and Huber, S.C. (1993) Identification of the major regulatory phosphorylation site in sucrose-phosphate synthase. Arch. Biochem. Biophys. 307, 248-252.
17. Marcus, F., Rittenhouse, J., Moberly, L., Edelstein, I., Hiller, E. and Rogers, D.T. (1988) Yeast (Saccharomyces cerevisiae) fructose-1,6-bisphosphatase. Properties of phospho and dephospho forms and of two mutants in which serine 11 has been changed by site-directed mutagenesis. J. Biol. Chem. 263, 6058-6062.
18. Reimholz, R., Geigenberger, P. and Stitt, M. (1994) Sucrose phosphate synthase is regulated, via metabolites and protein phosphorylation in potato tubers, in a manner analogous to the enzyme in leaves. Planta, 192, 480-488.
19. Rosahl, S., Schell, J. and Willmitzer, L. (1987) Expression of a tuber-specific storage protein in transgenic tobacco plants: demonstration of an esterase activity. EMBO J. 6, 1155-1159.
20. Siegl, G., MacKintosh, C. and Stitt, M. (1990) Sucrose-phosphate synthase is dephosphorylated by protein phosphatase 2A in spinach leaves. Evidence from the effects of okadaic acid and microcystin. FEBS Lett. 270, 198-202.
21. Sonnewald, U., Quick, W.P., MacRae, E., Krause, K.P. and Stitt, M. (1993) Purification, cloning and expression of spinach leaf sucrose-phosphate synthase in Escherichia coli. Planta, 189, 174-181.
22. Stitt, M., Huber, S.C. and Kerr, P.S. (1987) Control of photosynthetic sucrose formation. In Biochemistry of Plants, Volume 10 (Hatch, M.D. and Boardman, N.K., eds). New York: Academic Press, pp. 328-409.
23. Stitt, M., Wilke, I., Feil, R. and Heldt, H.W. (1988) Coarse control of sucrose-phosphate synthase in leaves: alterations of the kinetic properties in response to the rate of photosynthesis and the accumulation of sucrose. Planta, 174, 217-230.
24. Thorsness, P.E. and Koshland, D.E. Jr (1987) Inactivation of isocitrate dehydrogenase by phosphorylation is mediated by the negative charge of the phosphate. J. Biol. Chem. 262, 10422-10425.
25. Toroser, D. and Huber, S.C. (1997) Protein phosphorylation as a mechanism for regulation of sucrose-phosphate synthase under osmotic stress conditions. Plant Physiol. 114, 947-955.
26. Walker, J.L. and Huber, S.C. (1989) Purification and preliminary characterization of sucrose-phosphate synthase using monoclonal antibodies. Plant Physiol. 89, 518-524.
27. 4 phosphoenolpyruvate carboxylase from Sorghum. J. Biol. Chem. 267, 16759-16762.