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Comprehensive review of the genetic and molecular basis of self-incompatibility in Brassica oleracea and related species.
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Nasrallah JB Signal perception and response in the interactions of self-incompatibility in Brassica. Essays Biochem. 32:1997;143-160. Comprehensive review of the genetic and molecular basis of self-incompatibility in Brassica oleracea and related species.
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SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco
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Loss of callose in the stigma papillae does not affect the Brassica self-incompatibility phenotype
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Pollen-stigma adhesion in kale is not dependent on the self-(in)compatibility genotype
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A quantitative measurement of the binding force between pollen and the stigma in the first hour following pollination. Interestingly, both compatible and incompatible Brassica pollen was shown to bind with equal efficiencies to stigmas.
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Luu D-T, Heizmann P, Dumas C Pollen-stigma adhesion in kale is not dependent on the self-(in)compatibility genotype. Plant Physiol. 115:1997;1221-1230. A quantitative measurement of the binding force between pollen and the stigma in the first hour following pollination. Interestingly, both compatible and incompatible Brassica pollen was shown to bind with equal efficiencies to stigmas.
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Luu, D.-T.1
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An anther-specific gene encoded by an S locus haplotype of Brassica produces complementary and differentially regulated transcripts
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The male determinant of self-incompatibility in Brassica oleracea is located in the pollen coating
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An elegant bioassay was developed, that enabled purification and characterization of the pollen component of self-incompatibility. For this assay, the pollen coating was extracted from self-compatible and incompatible pollen. Reconstitution of this coating onto other pollen grains confirmed that male self-incompatibility can be controlled by factors in the pollen coat.
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Stephenson AG, Doughty J, Dixon S, Elleman C, Hiscock S, Dickinson HG The male determinant of self-incompatibility in Brassica oleracea is located in the pollen coating. Plant J. 12:1997;1351-1359. An elegant bioassay was developed, that enabled purification and characterization of the pollen component of self-incompatibility. For this assay, the pollen coating was extracted from self-compatible and incompatible pollen. Reconstitution of this coating onto other pollen grains confirmed that male self-incompatibility can be controlled by factors in the pollen coat.
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Cloning of PCP1, a member of a family of pollen coat protein (PCP) genes from Brassica oleracea encoding novel cysteine-rich proteins involved in pollen-stigma interactions
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Stanchev BS, Doughty J, Scutt CP, Dickinson H, Croy RRD Cloning of PCP1, a member of a family of pollen coat protein (PCP) genes from Brassica oleracea encoding novel cysteine-rich proteins involved in pollen-stigma interactions. Plant J. 10:1996;303-313.
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0032144968
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PCP-A1, a defensin-like Brassica pollen coat protein that binds the S locus glycoprotein, is the product of gametophytic gene expression
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Extracts of the pollen coating were shown to contain small peptides that bind to SLG, the stigma-specific component of the Brassica self-incompatibility response. Cloning the corresponding gene revealed sequences with similarity to peptides involved in anti-fungal defenses. Interestingly, the PCP gene was expressed after meiosis, suggesting that it is regulated by a haploid-specific promoter.
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Doughty J, Dixon S, Hiscock SJ, Willis AC, Parkin IAP, Dickinson HG PCP-A1, a defensin-like Brassica pollen coat protein that binds the S locus glycoprotein, is the product of gametophytic gene expression. Plant Cell. 10:1998;1333-1347. Extracts of the pollen coating were shown to contain small peptides that bind to SLG, the stigma-specific component of the Brassica self-incompatibility response. Cloning the corresponding gene revealed sequences with similarity to peptides involved in anti-fungal defenses. Interestingly, the PCP gene was expressed after meiosis, suggesting that it is regulated by a haploid-specific promoter.
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Identification of genes required for pollen-stigma recognition in Arabidopsis thaliana
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0032560126
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Lipids are required for directional pollen-tube growth
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A stigma mutant that lacks an oil-rich exudate was used to define lipid molecules required for pollen hydration and germination on Nicotiana stigmas. Interestingly, only certain lipids were found to have high levels of activity, and these lipids also rescued pollen hydration in Arabidopsis mutants that lack lipids in the pollen coating.
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Wolters-Arts M, Lush WM, Mariani C Lipids are required for directional pollen-tube growth. Nature. 392:1998;818-821. A stigma mutant that lacks an oil-rich exudate was used to define lipid molecules required for pollen hydration and germination on Nicotiana stigmas. Interestingly, only certain lipids were found to have high levels of activity, and these lipids also rescued pollen hydration in Arabidopsis mutants that lack lipids in the pollen coating.
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Molecular characterization of two Brassica napus genes related to oleosins which are highly expressed in the tapetum
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Characterization of anther-expressed genes encoding a major class of extracellular oleosin-like proteins in the pollen coat of Brassicaceae
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Localization of a Rho GTPase implies a role in tip growth and movement of the generative cell in pollen tubes
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Inhibition of pollen tube elongation by microinjected anti-Rop1Ps antibodies suggests a crucial role for Rho-type GTPases in the control of tip growth
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Pollen tube growth and the intracellular cytosolic calcium gradient oscillate in phase while extracellular calcium influx is delayed
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Fluorescent calcium reporter molecules were used to follow fluxes in the calcium gradient in pollen tubes. Importantly, the gradient fluctuations that occurred at the pollen tube tip were in phase with the pulsating growth of pollen tubes.
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Holdaway-Clarke TL, Feijo JA, Hackett GR, Kunkel JG, Hepler PK Pollen tube growth and the intracellular cytosolic calcium gradient oscillate in phase while extracellular calcium influx is delayed. Plant Cell. 9:1997;1999-2010. Fluorescent calcium reporter molecules were used to follow fluxes in the calcium gradient in pollen tubes. Importantly, the gradient fluctuations that occurred at the pollen tube tip were in phase with the pulsating growth of pollen tubes.
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Plant Cell
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A thorough examination of the genetic control of ovule development in Arabidopsis. Epistasis tests are performed with several developmental mutants, and a comprehensive model for ovule formation is presented.
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A creative investigation of the contribution of the female gametophyte to pollen tube guidance in Arabidopsis. A translocation heterozygote was used to show that pollen tubes target only ovules that contain a viable female gametophyte.
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