Insect immunity: Molecular cloning, expression, and characterization of cDNAs and genomic DNA encoding three isoforms of insect defensin in Aedes aegypti

Insect Molecular Biology

Volumn 8, Issue 1, 1999, Pages 107-118

  Lowenberger, Carl A ^a   Smartt, C T ^a   Bulet, P ^b   Ferdig, M T ^a   Severson, D W ^a,c   Hoffmann, J A ^b   Christensen, B M ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b INSTITUT DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

^c UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

Aedes aegypti; Defensin; Insect immunity

Indexed keywords

AMINO ACID SEQUENCE; CLONE; COMPLEMENTARY DNA; DEFENSIN; GENOMIC DNA; INOCULATION; INTRON; ISOFORM; MOLECULAR CLONING; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PREPRODEFENSIN; PRODEFENSIN; SIGNAL PEPTIDE;

AEDES AEGYPTI; BACTERIA (MICROORGANISMS); EUKARYOTA; HEXAPODA; INSECTA;

EID: 0033081711     PISSN: 09621075     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2583.1999.810107.x     Document Type: Article

References (54)

1. Beerntsen, B.T. and Christensen, B.M. (1990) Dirofilaria immitis: effect on hemolymph polypeptide synthesis in Aedes aegypti during melanotic encapsulation reactions against microfilariae. Exp Parasitol 71: 406-414.
2. Beerntsen, B.T., Severson, D.W., Klinkhammer, J.A., Kassner, V.A. and Christensen, B.M. (1995) Aedes aegypti: a quantitative trait locus (QTL) influencing filarial worm intensity is linked to QTL for susceptibility to other mosquito-borne pathogens. Exp Parasitol 61: 355-362.
3. Boman, H.G., Faye, I., Gudmundsson, G.H., Lee, J.Y. and Lidholm, D.A. (1991) Cell free immunity in Cecropia: a model system for antibacterial proteins. Eur J Biochem 201: 23-31.
4. Brey, P.T., Lee, W.J., Yamakawa, M., Koizumi, Y., Perrot, S., Francois, M. and Ashida, M. (1993) Role of the integument in insect immunity: epicuticular abrasion and induction of cecropin synthesis in cuticular epithelial cells. Proc Natl Acad Sci USA 90: 6275-6279.
5. Broekaert, W.F., Terras, F.R.G., Cammue, B.P.A. and Osborn, R.W. (1995) Plant defensins: novel antimicrobial peptides as components of the host defense system. Plant Physiol 108: 1353-1358.
6. Bulet, P., Cociancich, S., Reuland, M., Sauber, S., Bishoff, R., Hegy, G., Van Dorsselaer, A., Hetru, C. and Hoffmann, J.A. (1992) A novel insect defensin mediates the inducible antibacterial activity in larvae of the dragonfly, Aeschna cyanea (Paleoptera Odonata). Eur J Biochem 209: 977-984.
7. Casteels-Josson, K., Zhang, W., Capaci, T., Casteels, P. and Tempst, P. (1994) Acute transcriptional response of the honeybee peptide-antibiotics gene repertoire and required posttranslational conversion of the precursor structures. J Biol Chem 269: 28569-28575.
8. Chalk, R., Albuquerque, C.M.R., Ham, P.J. and Townson, H. (1995) Full sequence and characterization of two insect defensins: immune peptides from the mosquito Aedes aegypti. Proc R Soc Lond B 261: 217-221.
9. Chalk, R., Townson, H. and Ham, P.J. (1995) Brugia pahangi: the effects of cecropins on microfilariae in vitro and in Aedes aegypti. Exp Parasitol 80: 401-406.
10. Charlet, M., Chernysh, S., Philippe, H., Hetru, C., Hoffmann, J.A. and Bulet, P. (1996) Innate immunity: isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusc, Mytilus edulis. J Biol Chem 271: 21808-21813.
11. Cho, W.L., Fu, Y.C., Chen, C.C. and Ho, C.M. (1996) Cloning and characterization of cDNAs encoding the antibacterial peptide, Defensin A, from the mosquito, Aedes aegypti. Insect Biochem Mol Biol 26: 395-402.
12. Chomczynski, P. and Sacchi, N. (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analyt Biochem 162: 156-159.
13. Christensen, B.M. and Severson, D.W. (1993) Biochemical and molecular basis of mosquito susceptibility to Plasmodium and filarioid nematodes. Parasites and Pathogens of Insects, Vol. 1. Parasites (Beckage, N.B., Thompson, S.E. and Federici B.A., eds), pp. 245-266. Academic Press, San Diego.
14. Christensen, B.M. and Forton, K.F. (1986) Hemocyte-mediated melanization of microfilariae in Aedes aegypti. J Parasitol 72: 220-225.
15. Cociancich, S., Bulet, P., Hetru, C. and Hoffmann, J.A. (1994) The inducible antibacterial peptides of insects. Parasitol Today 10: 132-139.
16. Cociancich, S., Goyffon, M., Bontems, F., Bulet, P., Bouet, F., Mendez, A. and Hoffmann, J.A. (1993) Purification and characterization of a scorpion defensin, a 4 kDa antibacterial peptide presenting structural similarities with insect defensins and - scorpion toxins. Biochem Biophys Res Commun 194: 17-22.
17. Dimarcq, J.L., Hoffmann, D., Meister, M., Bulet, P., Lanot, R., Reichhart, J.M. and Hoffmann, J.A. (1994) Characterization and transcriptional profiles of a Drosophila gene encoding an insect defensin. Eur J Biochem 221: 201-209.
18. Dimarcq, J.L., Zachary, D., Hoffmann J.A., Hoffmann, D. and Reichhart, J.M. (1990) Insect immunity: expression of the two major inducible antibacterial peptides, defensin and diptericin, in Phormia terranovae. EMBO J 9: 2507-2515.
19. Dunn, P.E. (1986) Biochemical aspects of insect immunology. Annu Rev Entomol 31: 321-329.
20. Durbin, J.E., Swerdel, M.R. and Fallon, A.M. (1988) Identification of cDNAs corresponding to mosquito ribosomal protein genes. Biochim Biophys Acta 950: 182-192.
21. Ehret-Sabatier, L., Loew, D., Goyffon, M., Fehlbaum, P., Hoffmann, J.A., Dorsselaer, A. van and Bulet, P. (1996) Characterization of novel cysteine-rich antimicrobial peptides from scorpion blood. J Biol Chem 271: 29537-29544.
22. Evans, E.W. and Harmon, B.G. (1995) A review of antimicrobial peptides: defensins and related cationic peptides. Vet Clin Pathol 24: 109-116.
23. Fujiwara S., Imai, J., Fujiwara, M., Yaeshima, T., Kawashima, T. and Kobayashi, K. (1990) A potent antibacterial protein in royal jelly: purification and determination of the primary structure of royalisin. J Biol Chem 265: 11333-11337.
24. Ganz, T. and Lehrer, R.I. (1995) Defensins. Pharmac Ther 66: 191-205
25. Gwadz, R.W., Kaslow, D., Lee, J.Y., Maloy, W.L., Zasloff, M. and Miller, L.H. (1989) Effects of magainins and cecropins on the sporogonic development of malaria parasites in mosquitoes. Infect Immun 57: 2628-2633.
26. 1H nuclear magnetic resonance study of the solution conformation of an antibacterial protein, Sapecin. FEBS Lett 269: 413-420.
27. Hetru C., Bulet, P., Cociancich, S., Dimarcq, J.L., Hoffmann, D. and Hoffmann, J.A. (1994) Antibacterial peptides/polypeptides in the insect host defense. Phylogenetic Perspectives in Immunity: the Insect Host Defense (Hoffmann, J.A., Janeway, C.A. and Natori, S., eds), pp. 43-65. S.R.G. Landes Company, Austin, Texas.
28. Hetru, C., Hoffmann, D. and Bulet, P. (1998) Antimicrobial peptides from insects. Molecular Mechanisms of Immune Responses in Insects (Brey, P.T. and Hultmark, D., eds), pp. 40-66. Chapman and Hall, London.
29. Hoffmann, J.A. and Hetru, C. (1992) Insect defensins: inducible antibacterial peptides. Immunol Today 13: 411-415.
30. Hoffmann, J.A., Reichhart, J.M. and Hetru, C. (1996) Innate immunity in higher insects. Curr Opin Immunol 8: 8-13.
31. Hubert, F., Noel, T. and Roch, P. (1996) A member of the arthropod defensin family from edible Mediterranean mussels (Mytilus galloprovincialis). Eur J Biochem 240: 302-306.
32. Lackie, A.M. (1988) Immune mechanisms in insects. Parasitol Today 4: 98-105.
33. Lander E.S., Green, P., Abrahamson, J., Barlow, A., Daly, M., Lincoln, S. and Newberg, L. (1987) MapMaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174-181.
34. Lee Y.L., Chung T.J., Park, C.W., Hahn, Y., Chung, J.H., Lee, B.L., Han, D.M., Jumg, Y.H., Kim, S. and Lee, Y. (1996) Structure and expression of the tenecin 3 gene in Tenebrio molitor. Biochem Biophys Res Commun 218: 6-11.
35. Lehane, M.J., Wu, D. and Lehane, S.M. (1997) Midgut-specific immune molecules are produced by the blood-sucking insect Stomoxys calcitrans. Proc Natl Acad Sci USA 94: 11502-11507.
36. Li, J. and Christensen, B.M. (1990) Immune competence of Aedes trivittatus hemocytes as assessed by lectin binding. J Parasitol 76: 276-278.
37. Lidholm, D.A., Gudmundsson, G.H., Xanthopoulos, K.G. and Boman, H.G. (1987) Insect immunity: cDNA clones coding for the precursor forms of cecropins A and D, antibacterial proteins from Hyalophora cecropia. FEBS Lett 226: 8-12.
38. Lowenberger, C.A., Bulet, P., Charlet, M., Hetru, C., Hodgeman, B., Christensen, B.M. and Hoffmann, J.A. (1995) Insect immunity: isolation of three novel inducible antibacterial defensins from the vector mosquito, Aedes aegypti. Insect Biochem Mol Biol 25: 867-873.
39. Lowenberger, C.A. (1996) Insect immunology: mosquito immune responses. Lab Animal 25: 30-33.
40. Lowenberger, C., Ferdig, M.T., Bulet, P., Khalili, S., Hoffmann, J.A. and Christensen, B.M. (1996) Aedes aegypti: induced antibacterial proteins reduce the establishment and development of Brugia malayi. Exp Parasitol 83: 191-201.
41. Lowenberger, C.A., Kamal, S., Chiles, J., Paskewitz, S., Bulet, P., Hoffmann, J.A. and Christensen, B.M. (1999) Mosquito-Plasmodium interactions in response to immune activation of the vector. Exp Parasitol (in press).
42. Matsumoto, N., Okada, M., Takahashi, H., Qu, X.M., Nakajima, Y., Nakanishi, Y., Komano, H. and Natori, S. (1986) Molecular cloning of a cDNA and assignment of the C-terminal of sarcotoxin IA, a potent antibacterial protein of Sarcophaga peregrina. Biochem J 239: 717-722.
43. Matsuyama, K. and Natori, S. (1988) Molecular cloning of cDNA for sapecin and unique expression of the sapecin gene during the development of Sarcophaga peregrina. J Biol Chem 263: 17117-17121.
44. Moon H.J., Lee, S.Y., Kurata, S., Natori, S. and Lee, B.L. (1994) Purification and molecular cloning of cDNA for an inducible antibacterial protein from larvae of the Coleopteran, Tenebrio molitor. J Biochem 116: 53-58.
45. Penninckx, I.A.M.A., Eggermont, K., Terras, F.R.G., Thomma, BPHJ De Samblanx, G.W. Buchala, A., Metraux, J.P., Manners, J.M. and Broekaert, W.F. (1996) Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway. Plant Cell 8: 2309-2323.
46. Reichhart, J.M., Essrich, M., Dimarcq, J.L., Hoffmann, D., Hoffmann, J.A. and Lagueux, M. (1989) Insect immunity: isolation of cDNA clones corresponding to diptericin, an inducible antibacterial peptide from Phormia terranovae (Diptera) transcriptional profiles during immunization. Eur J Biochem 182: 423-427.
47. Richman, A.M., Bulet, P., Hetru, C., Barillas-Mury, C., Hoffmann, J.A. and Kafatos, F.C. (1996) Inducible immune factors of the vector mosquito Anopheles gambiae: biochemical purification of a defensin antibacterial peptide and molecular cloning of preprodefensin cDNA. Insect Molec Biol 5: 203-210.
48. Richman, A.M., Dimopoulos, G., Seeley, D. and Kafatos, F.C. (1997) Plasmodium activates the innate immune response of Anopheles gambiae mosquitoes. EMBO J 16: 6114-6119.
49. Richman, A.M. and Kafatos, F.C. (1995) Immunity to eukaryotic parasites in vector insects. Curr Opin Immunol 8: 14-19.
50. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.
51. Severson, D.W. and Kassner, V.A. (1995) Analysis of mosquito genome structure using graphical genotyping. Insect Mol Biol 4: 279-286.
52. Severson, D.W., Mori, A., Zhang, Y. and Christensen, B.M. (1993) Linkage map for Aedes aegypti using restriction fragment length polymorphisms. J Hered 84: 241-247.
53. Severson, D.W., Mori, A., Zhang, Y. and Christensen, B.M. (1994) Chromosomal mapping of two loci affecting filarial worm susceptibility in Aedes aegypti. Insect Mol Biol 3: 67-73.
54. Severson, D.W., Mori, A., Kassner, V.A. and Christensen, B.M. (1995) Comparative linkage maps for the mosquitoes, Aedes albopictus and Ae. aegypti, based on common RFLP loci. Insect Mol Biol 4: 41-45.