Isolation and culture of human neuromicrovascular endothelial cells for the study of angiogenesis in vitro

Journal of Neuroscience Research

Volumn 55, Issue 3, 1999, Pages 370-381

  Lamszus, Katrin ^a   Schmidt, Nils Ole ^a   Ergün, Süleyman ^a   Westphal, Manfred ^a  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

Author keywords

Blood brain barrier; Human brain endothelial cells; Neovascularization; Primary cultures

Indexed keywords

BASIC FIBROBLAST GROWTH FACTOR; SCATTER FACTOR; VASCULOTROPIN;

ANGIOGENESIS; ARTICLE; BLOOD BRAIN BARRIER; BRAIN BLOOD VESSEL; CELL ISOLATION; CELL PROLIFERATION; CELL ULTRASTRUCTURE; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; ISOLATION PROCEDURE; MICROCIRCULATION; PATHOPHYSIOLOGY; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; TISSUE CULTURE;

ARTERIOVENOUS MALFORMATIONS; CELLS, CULTURED; ENDOTHELIAL GROWTH FACTORS; ENDOTHELIUM, VASCULAR; GROWTH SUBSTANCES; HUMANS; LYMPHOKINES; NEOVASCULARIZATION, PATHOLOGIC; UMBILICAL VEINS; VASCULAR ENDOTHELIAL GROWTH FACTOR A; VASCULAR ENDOTHELIAL GROWTH FACTORS;

EID: 0033081502     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4547(19990201)55:3<370::AID-JNR12>3.0.CO;2-U     Document Type: Article

References (41)

1. Alby L, Auerbach R. 1984. Differential adhesion of tumor cells to capillary endothelial cells in vitro. Proc Natl Acad Sci USA 81:5739-5743.
2. Arap W, Pasqualini R, Ruoslahti E. 1998. Cancer treatment by targeted drug delivery to tumor vasculature in a mouse model. Science 279:377-380.
3. Arthur FE, Shivers RR, Bowman PD. 1987. Astrocyte-mediated induction of tight junctions in brain capillary endothelium: an efficient in vitro model. Dev Brain Res 36: 155-159.
4. Auerbach R, Alby L, Morrissey LW, Tu M, Joseph J. 1985. Expression of organ-specific antigens on capillary endothelial cells. Microvasc Res 29:401-411.
5. Azizkhan RG, Azizkhan JC, Zetter BR, Folkman J. 1980. Mast cell heparin stimulates migration of capillary endothelial cells in vitro. J Exp Med 152:931-944.
6. Biegel D, Spencer DD, Pachter JS. 1995. Isolation and culture of human brain microvessel endothelial cells for the study of blood-brain barrier properties in vitro. Brain Res 692:183-189.
7. Bowman PD, Betz AL, Wolinsky JS, Penney JB, Shivers RR, Goldstein GW. 1981. Primary culture of capillary endothelium from rat brain. In vitro 17:353-362.
8. DeBault LE, Cancilla PA. 1980. γ-Glutamyl transpeptidase in isolated brain endothelial cells: Induction by glial cells in vitro. Science 207:653-655.
9. Dorovini-Zis K, Prameya R, Bowman PD. 1991. Culture and characterization of microvascular endothelial cells derived from human brain. Lab Invest 64:425-436.
10. Duport S, Robert F, Muller D, Grau G, Rarisi L, Stoppini L. 1998. An in vitro blood-brain barrier model: cocultures between endothelial cells and organotypic brain slice cultures. Proc Natl Acad Sci USA 95:1840-1845.
11. Ergün S, Luttmer W, Fiedler W, Holstein AF. 1998. Functional expression and localization of vascular endothelial growth factor and its receptors in the human epididymis. Biol Reprod 58:160-168.
12. Ferrara N, Davis-Smyth T. 1997. The biology of vascular endothelial growth factor. Endocr Rev 18:4-25.
13. Folkman J. Shing Y. 1992. Angiogenesis. J Biol Chem 267:10931-10934.
14. Garlanda C, Dejana E. 1997 Heterogeneity of endothelial cells: Specific markers. Arterioscler Thromb Vasc Biol 17:1193-1202.
15. Grant DS, Tashiro KI, Segui-Real B, Yamada Y, Martin GR, Kleinman HK. 1989. Two different laminin domains mediate the differentiation of human endothelial cells into capillary-like structures in vitro. Cell 58:933-943.
16. Gupta K, Ramakrishnan S, Browne PV, Solovey A, Hebbel RP. 1997. A novel technique for culture of human dermal microvascular endothelial cells under either serum-free or serum-supplemented conditions: isolation by panning and stimulation with vascular endothelial growth factor. Exp Cell Res 230:244-251.
17. Hayflick L. 1965. The limited in vitro lifetime of human diploid cell strains. Exp Cell Res 37:614-636.
18. Jaffe EA, Nachman RL, Becker DG, Minick CR. 1973. Culture of human endothelial cells derived from human umbilical veins. J Clin Invest 52:2745-2756.
19. Kanda T, Iwasaki T, Yamawaki M, Ikeda K. 1997. Isolation and culture of bovine endothelial cells of endoneurial origin. J Neurosci Res 49:769-777.
20. Keegan A, Hill C, Kumar S, Phillips P, Schor A, Weiss J. 1982. Purified tumour angiogenesis factor enhances proliferation of capillary, but not aortic, endothelial cells in vitro. J Cell Sci 55:261-276.
21. Klein S, Roghani M, Rifkin DB. 1997. Fibroblast growth factors as angiogenesis factors: new insights into their mechanism of action. In: Goldberg ID, Rosen EM, editors. Regulation of angiogenesis. Basel: Birkhäuser-Verlag, p 159-192.
22. Kräling BM, Jimenez SA, Sorger T, Maul GG. 1994. Isolation and characterization of microvascular endothelial cells from the adult human dermis and from skin biopsies of patients with systemic sclerosis. Lab Invest 71:745-754.
23. Kubota Y, Kleinman HK, Martin GR, Lawley TJ. 1988. Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures. J Cell Biol 107:1589-1598.
24. Lamszus K, Schmidt NO, Jin L, Laterra J, Zagzag D, Way D, Witte M, Weinand M, Goldberg ID, Westphal M, Rosen EM. 1998. Scatter factor promotes motility of human glioma and neuromicrovascular endothelial cells. Int J Cancer 75:19-28.
25. Maher F, Vannucci SJ, Simpson IA. 1994. Glucose transporter proteins in brain. FASEB J 8:1003-1011.
26. Martin GM, Sprague CA, Epstein CJ. 1970. Replicative life span of cultivated human cells. Effect of donor's age, tissue and genotype. Lab Invest 23:86-92.
27. Meyer J, Rauh J, Galla HJ. 1991. The susceptibility of cerebral endothelial cells to astroglial induction of blood-brain barrier enzymes depends on their proliferative state. J Neurochem 57:1971-1977.
28. Pasqualini R, Ruoslahti E. 1996. Organ targeting in vivo using phage display peptide libraries. Nature 380:364-366.
29. Phillips P, Kumar P, Kumar S, Waghe M. 1979. Isolation and characterization of endothelial cells from rat and cow brain white matter. J Anat 129:261-272.
30. Risau W. 1991. Induction of blood-brain barrier endothelial cell differentiation. Ann NY Acad Sci 633:405-419.
31. Risau W. 1995. Differentiation of endothelium. FASEB J 9:926-933.
32. Rosen EM, Lamszus K, Laterra J, Polverini PJ, Rubin JS, Goldberg ID. 1997. HGF/SF in angiogenesis. Ciba Found Symp 212: 215-226.
33. Schneider EL, Mitsui Y. 1976. The relationship between in vitro cellular ageing and in vivo human age. Proc Natl Acad Sci USA 1976:92-96.
34. Springhorn JP, Madri JA, Squinto SP. 1995. Human capillary endothelial cells from abdominal wall adipose tissue: isolation using an anti-PECAM antibody. In Vitro Cell Dev Biol 31:473-181.
35. Suzuki F, Nagano T. 1986. Microvasculature of the human testis and excurrent duct system: Resin-casting and scanning electron-microscopic studies. Cell Tissue Res 243:79-89.
36. Tao-Cheng JH, Nagy Z, Brightman MW. 1987. Tight junctions of brain endothelium in vitro are enhanced by astroglia. J Neurosci 7:3293-3299.
37. Vinters HV, Reave S, Costello P, Girvin JP, Moore SA. 1987. Isolation and culture of cells derived from human cerebral microvessels. Cell Tissue Res 2657-667.
38. Westphal M, Cristante L, Grzyska U, Freckmann N, Zanella F, Zeumer H. Herrmann HD. 1994a. Treatment of cerebral arteriovenous malformations by neuroradiological intervention and surgical resection. Acta Neurochir 130:20-27.
39. Westphal M, Stavrou D, Nausch H, Valdueza JM, Herrmann HD. 1994b. Human neurocytoma cells in culture show characteristics of astroglial differentiation. J Neurosci Res 38:698-704.
40. Zetter BR. 1988. Endothelial heterogeneity: influence of vessel size, organ localization, and species specificity on the properties of cultured endothelial cells. In: Ryan US. editor. Endothelial cells. Volume 2. Boca Raton, FL: CRC. p 63-79.
41. Zhang HF, Ong WY, Leong SK, Garey LJ. 1997. Ultrastructural characteristics of blood vessels in the infant and adult human cerebral cortex. Histol Histopathol 12:85-97.