Biosynthesis and properties of an extracellular metalloprotease from the Antarctic marine bacterium Sphingomonas paucimobilis

Journal of Biotechnology

Volumn 70, Issue 1-3, 1999, Pages 53-60

  Turkiewicz, Marianna ^a   Gromek, Ewa ^a   Kalinowska, Halina ^a   Zielińska, Maria ^a  

^a LODZ UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

Antarctic marine bacteria; Metalloprotease; Psychrophilic enzymes

Indexed keywords

ACETIC ACID; BACTERIA; BIOSYNTHESIS; CATALYST ACTIVITY; COMPOSITION EFFECTS; HEMOGLOBIN; HYDROLYSIS; MARINE BIOLOGY; PH EFFECTS; PURIFICATION;

ETHYLENEDIAMINETETRAACETIC ACID; METALLOPROTEASE; PSYCHROPHILIC ENZYMES;

ENZYMES;

METALLOPROTEINASE;

ANTARCTICA; BIOSYNTHESIS; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SYNTHESIS; GROWTH; MARINE ENVIRONMENT; NONHUMAN; PRIORITY JOURNAL; SPHINGOMONAS PAUCIMOBILIS; TEMPERATURE;

EUPHAUSIA SUPERBA; SPHINGOMONAS PAUCIMOBILIS;

EID: 0033065920     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00057-7     Document Type: Conference Paper

References (18)

1. Adams M.W.W., Perler F.B., Kelly R.M. Extremozymes: expanding the limits of biocatalysis. Bio/Technology. 13:1995;662-668.
2. Anson M.L. Estimation of pepsin, trypsin papain and cathepsin with haemoglobin. J. Gen. Physiol. 22:1938;79-82.
3. Brenchley J.E. Psychrophilic micro-organisms and their cold-active enzymes. J. Ind. Microbiol. 17:1996;432-437.
4. Davail S., Feller G., Narinx E., Gerday C. Cold adaptation of proteins. J. Biol. Chem. 269:1994;17448-17453.
5. DeLong E.F. Marine microbial diversity. Trends Biotechnol. 15:1997;203-207.
6. Donachie, S.P., 1995. Ecophysiological description of marine bacteria from Admiralty Bay (Antarctica) and the digestive tracts of selected Euphausiidae, Philosophy Doctor Thesis, Polish Academy of Sciences, Warsaw.
7. Feller G., Narinx E., Arpigny J.L., Aittaleb M., Baise E., Genicot S., Gerday Ch. Enzymes from psychrophilic organisms. FEMS Microbiol. Rev. 18:1996;189-202.
8. Hamamoto T., Kaneda M., Horikoshi K., Kudo T. Characterization of a protease from a psychrotroph Pseudomonas fluorescens 114. Appl. Environ. Microbiol. 60:1994;3878-3880.
9. Hummel B.C.W. A modified spectrophotometric method for determination of chymotrypsin and thrombin. Can. J. Biochem. Physiol. 37:1959;1393-1400.
10. Kärst, U., Woehl, M., Czempinski, K., Schmid R.D., 1994. Characterization of extracellular hydrolases from marine psychrophilic bacteria. In: Seventh International Congress of Bacteriology and Applied Microbiology, Praha, Communicate MO122.
11. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
12. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:1951;165-275.
13. Margesin R., Schinner F. Characterization of a metalloprotease from psychrophilic Xanthomonas maltophilia. FEMS Microbiol. Lett. 79:1991;257-262.
14. Margesin R., Schinner F. Production and properties of an extracellular metalloprotease from a psychrophilic Pseudomonas fluorescens. J. Biotechnol. 24:1992;207-210.
15. Margesin R., Schinner F. A comparison of extracellular proteases from three psychrotrophic strains of Pseudomonas fluorescens. J. Gen. Appl. Microbiol. 38:1992;209-225.
16. Margesin R., Schinner F. Properties of cold-adapted micro-organisms and their potential role in biotechnology. J. Biotechnol. 33:1994;1-14.
17. Marshall C.J. Cold-adapted enzymes. Trends Biotechnol. 15:1997;359-363.
18. Stepanov V.M., Rudenskaya G.N. Proteinase affinity chromatography on bacitracin-Sepharose. J. Appl. Biochem. 5:1983;420-428.