Detection of pneumococcus from whole blood, buffy coat and serum samples by PCR during bacteremia in mice

APMIS

Volumn 107, Issue 6, 1999, Pages 601-605

  Salo, Paula ^b   Laitinen, Kirsi ^b   Leilnonen, Maija ^a  

^a NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

^b NONE

Author keywords

Mouse model; PCR; Pneumococcal bacteremia

Indexed keywords

DNA POLYMERASE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBIOTIC THERAPY; ARTICLE; BACTEREMIA; BLOOD ANALYSIS; BLOOD SAMPLING; DIAGNOSTIC ACCURACY; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STREPTOCOCCUS PNEUMONIAE; TREATMENT INDICATION;

EID: 0033063167     PISSN: 09034641     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1699-0463.1999.tb01598.x     Document Type: Article

References (17)

1. 1. Appelbaum PC. Antimicrobial resistance in Streptococcus pneumoniae: an overview. Clin Infect Dis 1992;15:77-83.
2. 2. Bruyn GAW, Zeger BJM, van Furth R. Mechanisms of host defense against infection with Streptococcus pneumoniae. Clin Infect Dis 1992; 14:251-62.
3. 3. Frimodt-Moller N, Thomsen VF The pneumococcus and the mouse protection test: inoculum, dosage and timing. Acta path microbiol immunol scand 1986;94:33-7.
4. 4. Hassan-King M, Baldch I, Secka O, Falade A, Greenwood B. Detection of Streptococcus pneumoniae DNA in blood cultures by PCR. J Clin Microbiol 1994:32:1721-4.
5. 5. Higuchi R. Simple and rapid preparation of samples for PCR. In: Erlich HA, editor: PCR Technology: Principles and Applications for DNA Amplification. Stockton Press, New York 1989, pp.31-8.
6. 6. Isaacman DJ, Karasic RB, Reynolds EA, Kost SI. Effect of number of blood cultures and volume of blood on detection of bacteremia in children. J Pediatr 1996; 128:190-5.
7. 7. Klein JO. Pneumococcal bacteremia in the young child. Am J Dis Child 1975; 129:1266-7.
8. 8. Klugman KP. Epidemiology, control and treatment of multiresistant pneumococci. Drugs 1996; 52:Suppl. 2:42-6.
9. 9. Korppi M, Koskela M, Jalonen E, Leinonen M. Serologically indicated pneumococcal respiratory infection in children. Scand J Infect Dis 1992;24;437-43.
10. 10. Kwok S, Higuchi R. Avoiding false positives in PCR. Nature 1989;339:237-8.
11. 11. Marton A, Gulyas M, Munoz R, Tomasz A. Extremely high incidence of antibiotic resistance in clinical isolates of Streptococcus pneumoniae in Hungary. J Infect Dis 1991;163:542-8.
12. 12. Paton JC, Andrew PW, Boulnois GJ, Mitchell TJ. Molecular analysis of the pathogenicity of Streptococcus pneumoniae: the role of pneumococcal proteins. Annu Rev Microbiol 1993;47:89-115.
13. 13. Roback MG, Tsai AK, Hanson KL. Delayed incubation of blood culture bottles: effect on recovery rate of Streptococcus pneumoniae and Haemophilus influenzae type b. Pediatr Emerg Care 1994; 10:268-72.
14. 14. Rudolph KM, Parkinson AJ, Black CM, Mayer LW. Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia. J Clin Microbiol 1993;31:2661-6.
15. 15. Salo P, Örtqvist Å, Leinonen M. Diagnosis of bacteremic pneumococcal pneumonia by amplification of pneumolysin gene fragment in serum. J Infect Dis 1995; 171:479-82.
16. 16. Yagupsky P, Notle FS. Quantitative aspects of septicemia. Clin Microbiol Rev 1990;3:269-79.
17. 17. Zhang Y, Isaacman DJ, Wadowsky RM, Rydquist-White J, Post CJ, Ehrlich GD Detection of Streptococcus pneumoniae in whole blood by PCR. J Clin Microbiol 1995;33:596-601.