Rapid screening of T-cell receptor (TCR) variable gene usage by multiplex PCR: Application for assessment of clonal composition

Tissue Antigens

Volumn 53, Issue 2, 1999, Pages 122-134

  Akatsuka, Y ^a   Martin, E G ^a   Madonik, A ^b   Barsoukov, A A ^a   Hansen, John A ^a,c  

^a Fred Hutchinson Cancer Research Center   (United States)

^b APPLIED BIOSYSTEMS   (United States)

^c UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

Multiplex PCR; Spectratyping; T cell cloning; TCR

Indexed keywords

ARTICLE; CONTROLLED STUDY; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC SCREENING; HUMAN; HUMAN CELL; MULTIGENE FAMILY; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; T LYMPHOCYTE RECEPTOR GENE;

CELL LINE; DNA PRIMERS; FLOW CYTOMETRY; HUMANS; POLYMERASE CHAIN REACTION; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; T-LYMPHOCYTES;

EID: 0033060598     PISSN: 00012815     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-0039.1999.530202.x     Document Type: Article

References (31)

1. 1. Rosenberg WM, Moss PA, Bell JI. Variation in human T-cell receptor Vβ and Jβ repertoire: analysis using anchor polymerase chain reaction. Eur J Immunol 1992: 22: 541-9.
2. 2. Rowen L, Koop BF, Hood L. The complete 685-kilobase DNA sequence of the human β T-cell receptor locus. Science 1996: 272: 1755-62.
3. 3. Choi YW, Kotzin B, Herron L, Callahan J, Marrack P, Kappler J. Interaction of Staphylococcus aureus toxin Superantigens with human T cells. Proc Natl Acad Sci U S A 1989: 86: 8941-5.
4. 4. Genevée C, Diu A, Nierat J et al. An experimentally validated panel of subfamily-specific oligonucleotide primers (Vα1-w29/Vβ1-w24) for the study of human T cell receptor variable V gene segment usage by polymerase chain reaction. Eur J Immunol 1992: 22: 1261-7.
5. 5. Loh EY, Elliott JF, Cwirla S, Lanier LL, Davis MM. Polymerase chain reaction with single-sided specificity: Analysis of cell receptor δ chain. Science 1989: 243: 217-20.
6. 6. Uematsu Y. A novel and rapid cloning method for the T-cell receptor variable region sequences. Immunogenetics 1991: 34: 174-8.
7. 7. Yoshioka T, Matsutani T, Iwagami S et al. Quantitative analysis of the usage of human T-cell receptor α and β chain variable regions by reverse dot blot hybridization. J Immunol Methods 1997: 201: 145-55.
8. 8. Chamberlain JS, Gibbs RA, Ranier JE, Caskey CT. Detection of gene deletions using multiplex polymerase chain reactions. In: Mathew C, ed. Methods in molecular biology: Protocols in human molecular genetics. Clifton, New Jersey: Humana Press, 1991: 299-312.
9. 9. Maslanka K, Piatek T, Gorski J, Yassai M, Gorski J. Molecular analysis of T-cell repertoires: Spectratypes generated by multiplex polymerase chain reaction and evaluated by radioactivity or fluorescence. Hum Immunol 1995: 44: 28-34.
10. 10. Monteiro J, Hingorani R, Choi IH, Silver J, Pergolizz R, Gregersen PK. Oligoclonality in the human CD8+ T-cell repertoire in normal subjects and monozygotic twins: Implications for studies of infectious and autoimmune disease. Molecular Med 1995: 1: 614-24.
11. 11. Födinger M, Buchmayer H, Schwarzinger I et al. Multiplex PCR for rapid detection of T-cell receptor-gamma chain gene rearrangements in patients with lymphoproliferative diseases. Brit J Haematol 1996: 94: 136-9.
12. 12. Arden B, Clark SP, Kabelitz D, Mak TW. Human T-cell receptor variable gene segment families. Immunogenetics 1995: 42: 455-500.
13. 13. Chothia C, Boswell DR, Lesk AM. The outline structure of the T-cell α/β receptor. EMBO J 1988: 7: 3745-55.
14. 14. Currier JR, Yassai M, Robinson MA, Gorski J. Molecular defects in TCRBV genes preclude thymic selection and limit the expressed TCR repertoire. J Immunol 1996: 157: 170-5.
15. 15. Chomczynski P. Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Analyt Biochem 1987: 162: 156-9.
16. 16. D'Aquila RT, Bechtel LJ, Videler JA, Eron JJ, Gorczyca P, Kaplan JC. Maximizing sensitivity and specificity of PCR by preamplification heating. Nucleic Acids Res 1991: 19: 3749.
17. 17. Riddell SR, Greenberg PD. The use of anti-CD3 and anti-CD28 monoclonal antibodies to clone and expand human antigen-specific T cells. J Immunol Methods 1990: 128: 189-201.
18. 18. Lefkovits I. Limiting dilution analysis. In: Lefkovits I, Pernis B, eds. Immunological method. New York: Academic Press, 1979.
19. 19. Gorski J, Yassai M, Zhu X, Kissella B, Keever C, Flomenberg N. Circulating T-cell repertoire complexity in normal individuals and bone marrow recipients analysed by CDR3 size spectratyping: correlation with immune status. J Immunol 1994: 152: 5109-19.
20. 20. Akatsuka Y, Cerveny C, Hansen JA. T-cell receptor clonal diversity following allogeneic marrow grafting. Human Immunol 1996: 48: 125-34.
21. 21. Davodeau F, Peyrat MA, Romagné F et al. Dual T-cell receptor β chain expression on human T lymphocytes. J Exp Med 1995: 181: 1391-8.
22. 22. Padovan E, Giachino C, Cella M, Valitutti S, Acuto O, Lanzavecchia A. Normal T lymphocytes can express two different T-cell receptor β chains: Implication for the mechanism of allelic exclusion. J Exp Med 1995: 181: 1587-91.
23. 23. Ferre RM, Schwarz MJ, Robertson NH et al. Development, multiplexing, and application of ARMS tests for common mutations in the CFTR gene. Am J Hum Genet 1992: 51: 251-62.
24. 24. Sarkar G, Cassady J, Bottema CDK, Sommer SS. Characterization of polymerase chain reaction amplification of specific alleles. Anal Biochem 1990: 186: 64-8.
25. 25. Saiki RK, Gelfand DH, Stoffel S et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988: 239: 487-91.
26. 26. Sunzeri FJ, Lee TH, Brownlee RG, Busch MP. Rapid simultaneous detection of multiple retrovirus DNA sequences using the polymerase chain reaction and capillary DNA chromatography. Blood 1991: 77: 879-86.
27. 27. Naumov YN, Hogan KT, Naumova EN, Pagel JT, Gorski J. A class I MHC-restricted recall response to a viral peptide in highly polyclonal despite stringent CDR3 selection: Implications for establishing memory T-cell repertoires in "real-world" conditions. J Immunol 1998: 160: 2842-52.
28. 28. Uhrberg M, Wernet P. Quantitative assessment of the human TCRBV repertoire by competitive PCR. J Immunol Methods 1996: 194: 155-68.
29. 29. Malissen M, Trucy J, Jouvin-Marche E, Cazenave PA, Scollary R, Malissen B. Regulation of TCR α and β gene allelic exclusion during T-cell development. Immunol Today 1994: 13: 315-20.
30. 30. Obata F, Tsunoda M, Ito K et al. A single universal primer for the T-cell receptor (TCR) variable genes enables enzymatic amplification and direct sequencing of TCRβ cDNA of various T-cell clones. Hum Immunol 1993: 36: 163-7.
31. 31. Moonka D, Loh EY. A consensus primer to amplify both α and β chains of the human T-cell receptor. J Immunol Methods 1994: 169: 41-51.