Sterol mutants of Chlamydomonas reinhardtii: Characterisation of three strains deficient in C24(28) reductase

Plant Physiology and Biochemistry

Volumn 37, Issue 4, 1999, Pages 241-249

  Salimova, Ekaterina ^a   Boschetti, Arminio ^b   Eichenberger, Waldemar ^b   Lutova, Ludmila ^a  

^a ST PETERSBURG STATE UNIVERSITY   (Russian Federation)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

C24(28) reductase; Chlamydomonas; Mutants; Nuclear transformation; Sterols

Indexed keywords

7 DEHYDROPORIFERASTEROL; C24(28) REDUCTASE; CATALYST ACTIVITY; DIGITONIN; FRACTIONATION; GAS CHROMATOGRAPHY; INSERTIONAL MUTAGENESIS; MASS SPECTROMETRY; MOLECULAR CLONING; MUTANT; NYSTATIN; PLASMID; PRECIPITATION; SOUTHERN BLOTTING; STEROL; TETRAENOL; THIN LAYER CHROMATOGRAPHY;

CHLAMYDOMONAS REINHARDTII;

EID: 0033057044     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0981-9428(99)80022-9     Document Type: Article

References (27)

1. Bard M., Wilson K.J., Thompson R.M. Isolation of sterol mutants in Chlamydomonas reinhardi: chromatographic analyses. Lipids. 13:1978;533-539.
2. Bolard J. How do polyene macrolide antibiotics affect the cellular membrane properties? Biochim. Biophys. Acta. 864:1986;257-304.
3. Debuchy R., Purton S., Rochaix J.D. The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO J. 8:1989;2803-2809.
4. Eichenberger W. Sterine in Chlamydomonas reinhardi (Grünalgen). Chimia. 30:1976;75-78.
5. Eichenberger W. Synthesis of steroids in vascular plants. Zaborsky O.R. Handbook of Biosolar Resources, vol. 1, part 1. 1982;445-450 CRC Press Inc., Boca Raton.
6. Eichenberger W., Menke W. Sterole in Blättern und Chloroplasten. Z. Naturforsch. 21b:1966;859-867.
7. 7-sterol-C-5-desaturase from yeast. Plant J. 8:1995;407-416.
8. Gealt A.M., Adler J.H., Nes W.R. The sterols and fatty acids from purified flagella of Chlamydomonas reinhardtii. Lipids. 16:1981;133-136.
9. Goldstein J.L., Brown M.S. Regulation of mevalonate pathway. Nature. 343:1990;425-430.
10. Gorman D.S., Levine R.P. Cytochrome f and plastocyanin: Their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc. Natl. Acad. Sci. USA. 54:1965;1665-1669.
11. Grunwald C. Functions of sterols. Phil. Trans. R. Soc. Lond. B Biol. Sci. 284:1978;541-558.
12. Heftmann E. Steroid Biochemistry. 1970;Academic Press, New York.
13. Kindle K.L. High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA. 87:1990;1228-1232.
14. Klahre U., Noguchi T., Fujioka S., Takatsuto S., Yokota T., Nomura T., Yoshida S., Chua N.H. The Arabidopsis diminuto/dwarf1 gene encodes a protein involved in sterol biosynthesis. Plant Cell. 10:1998;1677-1690.
15. Lai M.H., Bard M., Pierson C.A., Alexander J.F., Goebl M., Carter G.T., Kirch D.R. The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway. Gene. 140:1994;41-49.
16. Lees N.D., Skaggs B., Kirsch D.R., Bard M. Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae - a review. Lipids. 30:1995;221-226.
17. Patterson G.W. Sterols of some green algae. Comp. Biochem. Physiol. 47B:1974;453-457.
18. Patterson G.W. Steroids of algae. Zaborsky O.R. Handbook of Biosolar Resources, vol. 1, part 1. 1982;433-444 CRC Press Inc., Boca Raton.
19. Purton S., Rochaix J.D. Characterisation of the ARG7 gene of Chlamydomonas reinhardtii and its application to nuclear transformation. Eur. J. Phycol. 30:1995;141-148.
20. Rochaix J.D. Chlamydomonas reinhardtii as the photosynthetic yeast. Annu. Rev. Genet. 29:1995;209-230.
21. Rochaix J.D., Mayfield S., Goldschmidt-Clermont M., Erickson J. Molecular biology of Chlamydomonas. Shaw C.H. Plant Molecular Biology. A Practical Approach. 1988;253-275 IRL Press, Oxford.
22. Rodriguez R.J., Low C., Bottema C.D.K., Parks L.W. Multiple functions for sterols in Saccharomyces cerevisiae. Biochim. Biophys. Acta. 837:1985;336-343.
23. Sager R., Granick S. Nutritional control of sexuality in Chlamydomonas reinhardi. J. Gen. Physiol. 37:1954;729-742.
24. Sambrook J., Frisch E.F., Maniatis T. Molecular Cloning. A Laboratory Manual. 2nd :1989;Cold Spring Harbor Laboratory Press, New York.
25. + gene encodes a protein similar to the chicken lamin B receptor and is implicated in pleiotropic drug-sensitivity, divalent cation-sensitivity, and osmoregulation. Mol. Biol. Cell. 3:1992;263-273.
26. Tam L.W., Lefebvre P.A. Insertional mutagenesis and isolation of tagged genes in Chlamydomonas. Methods Cell Biol. 47:1995;519-523.
27. Wang M.M., Sugar I.P., Chong P.L.G. Role of the sterol superlattice in the partitioning of the antifungal drug nystatin into lipid membranes. Biochemistry. 37:1998;11797-11805.