Three dimensional protein localization using high voltage electron microscopy

Acta Histochemica et Cytochemica

Volumn 32, Issue 1, 1999, Pages 35-43

  Martone, Maryann E ^a,b   Deerinck, Thomas J ^a   Young, Stephen J ^a   Ellisman, Mark H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Dendritic spines; Electron tomography; Endoplasmic reticulum; Fluorescence photooxidation; Phalloidin

Indexed keywords

EID: 0033054884     PISSN: 00445991     EISSN: None     Source Type: Journal    
DOI: 10.1267/ahc.32.35     Document Type: Conference Paper

References (43)

1. Agard, D. A., Koster, A. J. and Braufeld, M. B.: Automated data collection for electron tomography. Proc. Electron Microsc. Soc. Am. 27; 1044-1045, 1992.
2. Arii, T. and Hama, K.: Method of extracting 3-dimensional information from HVTEM stereo images of biological materials. J. Electron Microsc. 36; 177-195, 1987.
3. Crowther, R. A., DeRosier, D. J. and Klug, A.: The reconstruction of a three-dimensional structure from projections and its application to electron microscopy. Proc. R. Soc. London A 317; 319-340, 1970.
4. Deerinck, T. J., Martone, M. E., Lev-Ram, V., Green, D. P. L., Tsien, R. Y. and Ellisman, M. H.: Fluorescence photooxidation with eosin: a method for high resolution Immunolocalization and in situ hybridization detection for light and electron microscopy. J. Cell Biol. 126; 901-910, 1994.
5. Dierksen, K., Typke, D., Hegerl, R. and Baumeister, W.: Towards automatic electron tomography II: Implementation of autofocus and low-dose procedures. Ultramicroscopy 40; 71-87, 1993.
6. Fan, G. Y., Mercurio, P. J., Young, S. J. and Ellisman, M. H.: Telemicroscopy. Ultramicroscopy 52; 499-503, 1993.
7. Fan, G. Y., Young, S. J., Miller, P. and Ellisman, M. H.: Conditions for electron tomographic data acquisition. J. Electron Microsc. 44; 15-21, 1995.
8. Favard, P. and Carasso, N.: The preparation and observation of thick biological sections in the high voltage electron microscope. J. Microsc. 97; 59-81, 1972.
9. Forbes, M. S., Plantholt, B. A. and Sperelakis, N.: Cytochemical staining procedures selective for sarcotubular systems of muscle: modifications and applications. J. Ultrastruc. Res. 60; 306-327, 1977.
10. Frank, J.: Electron tomography: three-dimensional imaging with the transmission electron microscope, Plenum Press, New York, 1992.
11. Hama, K. and Porter, K. R.: An application of high voltage electron microscopy to the study of biological materials. J. Microsc. 8; 149-158, 1969.
12. Harris, K. M., Jensen, F. E. and Tsao, B.: Three-dimensional structure of dendritic spines and synapses in rat hippocampus (CA1) at postnatal day 15 and adult ages: implications for the maturation of synaptic physiology and long-term potentiation. J. Neurosci. 12; 2685-2705, 1992.
13. Hessler, D., Young, S. J. and Ellisman, M. H.: A flexible environment for the visualization of three-dimensional biological structures. J. Struct. Biol. 116; 113-119, 1996.
14. Huang, S., Deerinck, T. J., Ellisman, M. H. and Spector, D. L.: In vivo analysis of the stability and transport of nuclear poly(A)+ RNA. J. Cell Biol. 126; 877-899, 1994.
15. Huang, S., Deerinck, T. J., Ellisman, M. H. and Spector, D. L.: The dynamic organization of the perinucleolar compartment in the cell nucleus. J. Cell Biol. 137; 965-974, 1997.
16. Kremer, J. R., Mastronarde, D. N. and McIntosh, J. R.: Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116; 71-76, 1996.
17. Ladinsky, M. S., Kremer, J. R., Furcinitti, P. S., McIntosh, J. R. and Howell, K. E.: HVEM tomography of the trans-Golgi network: structural insights and identification of a lace-like vesicle coat. J. Cell Biol. 127; 29-38, 1994.
18. Leith, A.: Computer visualization of volume data in electron tomography. In "Electron Tomography", ed. by J. Frank, Plenum Press, New York, 1992, pp. 215-236.
19. Lenzi, D., Runyeon, J. W., Crum, J., Ellisman, M. H. and Roberts, W. M.: Synaptic vesicle populations in saccular hair cells reconstructed by electron tomography. J. Neurosci. 19; 119-132, 1999.
20. Lindsey, J. D. and Ellisman, M. H.: The neuronal endomembrane system I. Direct links between rough endoplasmic reticulum and the cis element of the Golgi apparatus. J. Neurosci. 5; 3111-3123, 1985.
21. Maranto, A.: Neuronal mapping: a photooxidation reaction makes Lucifer Yellow useful for electron microscopy. Science 217; 953-955, 1982.
22. Marko, M., Leith, A. and Parsons, D.: Three-dimensional reconstruction of cells from serial sections and whole-cell mounts using multilevel contouring of stereo micrographs. J. Elect. Microsc. Tech. 9; 395-411, 1988.
23. Martone, M. E., Zhang, Y., Simpliciano, V. M., Carragher, B. O. and Ellisman, M. H.: Three dimensional visualization of the smooth endoplasmic reticulum in Purkinje cell dendrites. J. Neurosci. 13; 4636-4646, 1993.
24. Martone, M. E., Jones, Y. Z., Young, S. J., Ellisman, M. H. and Zivin, J. A.: Modification of postsynaptic densities following transient cerebral ischemia: a quantitative and three dimensional ultrastructural study. J. Neurosci. 1999 (in press)
25. Mastronarde, D. N.: Dual-axis tomography: an approach with alignment methods that preserve resolution. J. Struct. Biol. 120; 343-352, 1997.
26. Misteli, T. and Spector, D. L.: Applications of the green fluorescent protein in cell biology and biotechnology. Nature Biotech. 15; 961-964, 1997.
27. Pagano, R. E., Sepanski, M. A. and Martine, O. C.: Molecular trapping of a fluorescent ceramide analogue at the Golgi apparatus of fixed cells: interaction with endogenous lipids provides a trans-Golgi marker for both light and electron microscopy. J. Cell Biol. 109; 2067-2079, 1989.
28. Penczek, P., Marko, M., Buttle, K. and Frank, J.: Double-tilt electron tomography. Ultramicroscopy 60; 393-410, 1995.
29. Perkins, G., Renkin, C., Martone, M. E., Young, S. J., Ellisman, M. and Frey, T.: Electron tomography of neuronal mitochondria: three-dimensional structure and organization of cristae and membrane contacts. J. Struct. Biol. 119; 260-272, 1997.
30. Perkins, G. A., Renken, C. W., Song, J. Y., Frey, T. G., Young, S. J., Lamont, S., Martone, M. E., Lindsey, S. and Ellisman, M. H.: Electron tomography of large, multicomponent biological structures. J. Struct. Biol. 120; 219-227, 1997.
31. Rademacher, M.: Three-dimensional reconstruction of single particles from random and nonrandom tilt series. J. Elect. Microsc. Tech. 9; 359-394, 1988.
32. Rambourg, A. and Clermont, Y.: Tridimensional structure of the Golgi apparatus in type A ganglion cells of the rat. Am. J. Anat. 176; 393-409, 1986.
33. Reider, C. L., Rupp, G. and Bowser, S. S.: Microscopy of semithick sections: advantages for research. J. Electron Microsc. Tech. 2; 11-28, 1985.
34. Sandell, J. H. and Masland, R. H.: Photoconversion of some fluorescent markers to a diaminobenzidine product. J. Histochem. Cytochem. 36; 555-560, 1988.
35. Shoop, R. D., Martone, M. E., Yamada, N., Ellisman, M. H. and Berg, D. K.: Neuronal acetylcholine receptors with α7 subunits are concentrated on somatic spines for synaptic signaling. J. Neurosci. 19; 692-704, 1999.
36. Skoglund, U., Ofverstedt, L. G., Burnett, R. M. and Bricogne, G.: Maximum entropy three-dimensional reconstruction with deconvolution of the contrast function: a test application with adenovirus. J. Struct. Biol. 117; 173-188, 1996.
37. Sommer, J. R. and Waugh, R. A.: The ultrastructure of the mammalian cardiac muscle cell-with special emphasis on the tubular membrane systems. A review. Am. J. Pathol. 82; 192-232, 1976.
38. Soto, G. E., Young, S. J., Martone, M. E., Deerinck, T. J., Lamont, S., Carragher, B. O., Hama, K. and Ellisman, M. H.: Serial section electron tomography: a method for three-dimensional reconstruction of large structures. Neuroimage 1; 230-243, 1994.
39. Spacek, J. and Harris, K. M.: Three-dimensional organization of smooth endoplasmic reticulum in hippocampal CA1 dendrites and dendritic spines of the immature and mature rat. J. Neurosci. 17; 190-203, 1997.
40. Takizawa, P. A., Yucel, J. K., Veit, B., Faulkner, DJ., Deerinck, T., Soto, G., Ellisman, M. and Malhotra, V.: Complete vesiculation of Golgi membranes and inhibition of protein transport by a novel sea sponge metabolite ilimaquinone. Cell 73; 1079-1090, 1993.
41. Wilson, C. J.: Understanding the neostriatal microcircuitry: high voltage electron microscopy. Microsc. Res. Tech.29; 368-380, 1994.
42. Wilson, C. J., Mastronarde, D. N., McEwen, B. and Frank, J.: Measurement of neuronal surface area using high-voltage electron microscope tomography. NeuroImage 1; 11-22, 1992.
43. Young, S. J., Fan, G. G. Y., Hessler, D., Lamont, S., Elvins, T. T., Hadida-Hassan, M., Hanyzewski, G. A., Durkin, J. W., Hubbard, P., Kindlmann, G., Wong, E., Greenberg, D., Karin, S. and Ellisman, M. H.: Implementing a collaboratory for microscopic digital anatomy. Int. J. Supercomputer Applications and High Performance Computing 10; 170-181, 1996.