Bgugaine, a pyrrolidine alkaloid from Arisarum vulgare, is a strong hepatotoxin in rat and human liver cell cultures

Toxicology Letters

Volumn 104, Issue 3, 1999, Pages 239-248

  Rakba, Nafissa ^a   Melhaoui, Ahmed ^c   Loyer, Pascal ^a   Guy Delcros, Jean ^b   Morel, Isabelle ^a   Lescoat, Gérard ^a  

^a INSERM   (France)

^b CNRS   (France)

^c Detoxication Reparation T ^*

Author keywords

Arisarum vulgare; Bgugaine; Liver cell cytolysis; Pyrrolidine alkaloid

Indexed keywords

ALKALOID; DNA FRAGMENT; LACTATE DEHYDROGENASE; PYRROLIDINE DERIVATIVE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOLYSIS; CYTOTOXICITY; DNA DAMAGE; HEPATOBLASTOMA; HUMAN; HUMAN CELL; LIVER CELL CULTURE; LIVER TOXICITY; MALE; NONHUMAN; PLANT; PRIORITY JOURNAL; RAT;

ALKALOIDS; ANIMALS; CELLS, CULTURED; DNA; DNA DAMAGE; ELECTROPHORESIS; EPITHELIAL CELLS; FLOW CYTOMETRY; HEPATOBLASTOMA; HUMANS; LIVER DISEASES; LIVER NEOPLASMS; MALE; PLANT EXTRACTS; PLANTS, TOXIC; PYRROLIDINES; RATS; RATS, SPRAGUE-DAWLEY; TUMOR CELLS, CULTURED;

ANIMALIA; ARISARUM; ARISARUM VULGARE;

EID: 0033052598     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-4274(98)00375-0     Document Type: Article

References (24)

1. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
2. Bégué J.M., Guguen-Guillouzo C., Pasdeloup N., Guillouzo A. Prolonged maintenance of active cytochrome P-450 in adult rat hepatocytes cocultured with another liver cell type. Hepatology. 4:1984;839-842.
3. Csallany A.S., Guan D.M., Manwaring J.D., Addis P.B. Free malondialdehyde determination in tissues by high performance liquid chromatography. Anal. Biochem. 142:1984;277-283.
4. 2 cell line. Biochem. Pharmacol. 56:1998;431-437.
5. Dong Z., Saikumar P., Weinberg J.M., Venkatachalam M.A. Internucleosomal DNA cleavage triggered by plasma membrane damage during necrotic cell death. Involvement of serine but not cysteine proteases. Am. J. Pathol. 151(5):1997;1205-1213.
6. Guguen C., Guillouzo A., Boisnard M., Le Cam A., Bourel M. Etude ultrastructurale de monocouches d'hépatocytes de rat adulte cultivés en présence d'hémisuccinate d'hydrocortisone. Biol. Gastroenterol. 8:1975;223-231.
7. Guguen-Guillouzo C., Clément B., Baffet G., Beaumont C., Morel-Chany E., Glaise D., Guillouzo A. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes cocultured with another liver epithelial cell type. Exp. Cell. Res. 134:1983;47-54.
8. Guillouzo A., Beaune P., Gascoin M.N., Bégué J.M., Campion J.P., Guengerich F.P., Guguen-Guillouzo C. Maintenance of cytochrome P-450 in cultured adult human hepatocytes. Biochem. Pharmacol. 34:1985;2991-2995.
9. Guillouzo A. Hepatotoxicity. Frazier J.M. In vitro Toxicology Testing. Applications to safety evaluation. 1991;45-83 Marcel Dekker, New York.
10. Grisham J.W. Cell types in long term propagable cultures of rat liver. Ann. NY Acad. Sci. 349:1980;128-137.
11. Knowles B.B., Howe C.C., Aden D.P. Human hepatocellular carcinoma cell lines secrete the major proteins and hepatitis B antigen. Science. 209:1980;497-499.
12. Kerr J.F.R., Harmon B.V. Definition and incidence of apoptosis: an histological perspective. In Apoptosis: the molecular basis of cell death. 1991;5-19 Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
13. Lennon S.V., Martin S.J., Cotter T.G. Dose-dependent induction of apoptosis in human tumour cell lines by widely diverging stimuli. Cell. Proli. 24:1991;203-214.
14. Melhaoui A., Jossang A., Bodo B. Structure of irniine, a pyrrolidine alkaloid from Arisarum vulgare. J. Nat. Prod. 55:1992;950-952.
15. Melhaoui A., Mallea M., Jossang A., Bodo B. Antibiotic and antifungal pyrrolidine alkaloids from Arisarum vulgare. Nat. Prod. Lett. 2:1993;237-242.
16. Melhaoui A., Bodo B. Inirgaine and N-methylirnigaine two new piperidinol alkaloids from tubers of Arisarum vulgare. Nat. Prod. Lett. 7:1995;101-108.
17. Meyer B.N., Ferrigni N.R., Putman J.E., Jacobsen L.B., Nichols D.E., Mc Laughlin J.L. A convenient general bioassay for active plant constituents. Planta. Med. 45:1982;31-34.
18. Morel-Chany E., Guillouzo C., Tringal G., Szajnert M.F. Spontaneous neoplastic transformation in vitro of epithelial cell strain of rat liver: cytology, growth and enzymatic activities. Eur. J. Cancer. 14:1978;1341-1352.
19. Morel I., Lescoat G., Cillard J., Pasdeloup N., Brissot P., Cillard P. Kinetic evaluation of free malondialdehyde and enzyme leakage as indices of iron damage in rat hepatocyte cultures. Involvement of free radicals. Biochem. Pharmacol. 39:1990;1647-1655.
20. Mosmann T.J. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J. Immunol. Methods. 65:1983;55-57.
21. Ratanasavanh D., Baffet G., Latinier M.F., Rissel M., Guillouzo A. Use of hepatocyte cocultures in the assesment of drug toxicity from chronic exposure. Xenobiotica. 18:1988;765-771.
22. 2+ on enzymatic dispersion of isolated, perfused liver. Exp. Cell. Res. 74:1972;450-454.
23. Seglen O. Preparation of rat liver cells. 3. Enzymatic requirements for tissue dispersion. Exp. Cell. Res. 82:1973;391-398.
24. Williams G.M., Gun J.M. Long-term cell culture of adult rat liver epithelial cells. Exp. Cell. Res. 89:1974;139-142.