An efficient Agrobacterium tumefaciens-mediated transformation and regeneration system for cotyledons of spinach (Spinacia oleracea L.)

Plant Cell Reports

Volumn 18, Issue 7-8, 1999, Pages 640-645

  Zhang, H X ^a   Zeevaart, J A D ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

Cotyledon; Green fluorescent protein; Regeneration; Spinach (Spinacia oleracea L.); Transformation

Indexed keywords

BENZYLADENINE; CAULIFLOWER MOSAIC VIRUS; CULTURE MEDIUM; GENE EXPRESSION; GENETIC TRANSFORMATION; GREEN FLUORESCENT PROTEIN; INDOLEBUTYRIC ACID; KANAMYCIN; NAPHTHALENEACETIC ACID; NEOMYCIN PHOSPHOTRANSFERASE II; NORTHERN BLOTTING; PLANT GENOME; PROMOTER REGION; REPORTER GENE; SOUTHERN BLOTTING; TRANSGENIC PLANT; WESTERN BLOTTING;

AGROBACTERIUM TUMEFACIENS; SPINACIA OLERACEA;

EID: 0033050334     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002990050635     Document Type: Article

References (24)

1. Al-Khayri JM, Huang FG, Morelock TE (1991a) Regeneration of spinach from leaf callus. Hort Sci 26:913-914
2. Al-Khayri JM, Huang FG, Morelock TE, Busharar TA, Gbur EE (1991b) Genotype-dependent response of spinach cultivars to in vitro callus induction and plant regeneration. Plant Sci 78:121-127
3. Al-Khayri JM, Huang FG, Morelock TE, Busharar TA (1992a) In vitro plant regeneration of spinach from mature seed-derived callus. In Vitro Cell Dev Biol 28:64-66
4. Al-Khayri JM, Huang FG, Morelock TE, Busharar TA (1992b) Stimulation of shoot regeneration on spinach callus by gibberellic acid. Hort Sci 27:1046
5. Al-Khayri JM (1995) Genetic transformation in Spinacia oleracea L. (spinach). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol V/34. Springer, Berlin Heidelberg New York, pp 279-288
6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-252
7. Davis SJ, Vierstra RD (1998) Soluble, highly fluorescent variants of green fluorescent protein (GFP) for use in higher plants. Plant Mol Biol 36:521-528
8. Ezura H, Harberd NP (1995) Endogenous gibberellin levels influence in-vitro shoot regeneration in Arabidopsis thaliana (L.) Heynh. Planta 197:301-305
9. Fullner KJ, Cano Lara J, Nester EW (1996) Pillus assembly by Agrobacterium T-DNA transfer genes. Science 273:1107-1109
10. Hangarter RP, Stasinopoulos TC (1991) Repression of plant-tissue culture-growth by light is caused by photochemical change in the culture medium. Plant Sci 79:253-257
11. Jefferson RA, Kavanagh TA, Bevan MW (1987) Gus fusions: β-glucuronidase, a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901-3907
12. Knoll KA, Short KC, Curtis IS, Power JB, Davey MR (1997) Shoot regeneration from cultured root explants of spinach (Spinacia oleracea L.): a system for Agrobacterium transformation. Plant Cell Rep 17:96-101
13. Molvig L, Rose RJ (1994) A regeneration protocol for Spinacia oleracea using gibberellic acid. Aust J Bot 42:763-769
14. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-495
15. Powell Abel P, Nelson RS, De B, Hoffmann N, Rogers SG, Fraley RT, Beachy RN (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738-743
16. Sakaki H, Arato T, Takahashi K (1994) Regeneration of spinach plant from hypocotyl tissue cultured in vitro. J Hokkaido Univ Educ 45:31-35
17. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
18. Stasinopoulos TC, Hangarter RP (1990) Preventing photochemistry in culture media by long-pass light filters alters growth of cultured tissues. Plant Physiol 93:1365-1369
19. Strommer J, Gregerson R, Vayda M (1993) Isolation and characterizaton of plant mRNA. In: Glik BR, Thompson JE (eds) Methods in plant molecular biology and biotechnology. CRC Press, Boca Raton, Fla, pp 49-66
20. Thorpe TA, Meijer DD (1975) Effect of gibberellic acid in starch metabolism in tobacco callus cultured under shoot-forming conditions. Phytomorphology 25:238-245
21. Xiao X-G, Branchard M (1993) Embryogenesis and plant regeneration of spinach (Spinacia oleracea L.) from hypocotyl segments. Plant Cell Rep 13:69-71
22. Xiao X-G, Branchard M (1995) In vitro high frequency plant regeneration from hypocotyl and root segments of spinach by organogenesis. Plant Cell Tissue Organ Cult 42:239-244
23. Xu Y-L, Li L, Wu K, Peeters AJM, Gage DA, Zeevaart JAD (1995) The GA5 locus of Arabidopsis thaliana encodes a multifunctional gibberellin 20-oxidase: molecular cloning and functional expression. Proc Natl Acad Sci USA 92:6640-6644
24. Yang YM, Al-Khayri JM, Anderson EJ (1997) Transgenic spinach plants expressing the coat protein of cucumber mosaic virus. In Vitro Cell Dev Biol Plant 33:200-204