Production of low-lactose milk by ectopic expression of intestinal lactase in the mouse mammary gland

Nature Biotechnology

Volumn 17, Issue 2, 1999, Pages 160-164

  Jost, Bernard ^a   Vilotte, Jean Luc ^b   Duluc, Isabelle ^a   Rodeau, Jean Luc ^c   Freund, Jean Noël ^a  

^a INSERM   (France)

^b CEMAGREF   (France)

^c CNRS   (France)

Author keywords

Fat globules; Lactose maldigestion; Transgenic mice

Indexed keywords

ALPHA LACTALBUMIN; COMPLEMENTARY DNA; FAT DROPLET; HYDROLASE; INTESTINE ENZYME; LACTASE; LACTOSE; PHLORIZIN;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME SYNTHESIS; FEMALE; GENE CONTROL; HYBRID GENE; LACTATION; MAMMARY GLAND; MILK; MILK PRODUCTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRANSGENIC MOUSE;

ANIMALS; BASE SEQUENCE; BETA-GALACTOSIDASE; BLOTTING, WESTERN; CHROMATOGRAPHY, THIN LAYER; DNA, COMPLEMENTARY; FEMALE; GENE EXPRESSION; INTESTINES; LACTASE; LACTOSE; LACTOSE INTOLERANCE; MAMMARY GLANDS, ANIMAL; MICE; MICE, TRANSGENIC; MILK;

ANIMALIA; MUS MUSCULUS; RODENTIA;

EID: 0033045265     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/6158     Document Type: Article

References (42)

1. Mercier, J.C. 1986. Genetic engineering applied to milk producing animals: some expectations, pp. 122-131 in Exploiting technologies in animal breeding-genetic development. Smith, C., King, J.W.B., and McKays, J.C. (eds.). Oxford University Press, Oxford, UK.
2. National Dairy Council. 1985. Nutritional implications of lactose and lactase activity. Dairy Counc. Dig. 56:25-30.
3. Mepham, T.B. 1987. The composition of milks, pp. 30-50 in Physiology of lactation. Mepham, T.B. (ed.). Open University Press, Milton Keynes, PA.
4. Sahi, T. 1994. Genetics and epidemiology of adult-type hypolactasia. Scand. J. Gastroenterol. 29:7-20.
5. Suarez, F.L., Savaiano, D.A., and Levitt, M.D. 1995. The treatment of lactose intolerance. Aliment. Phamacol. Ther. 9:589-597.
6. Villako, K. and Maaroos, H. 1994. Clinical picture of hypolactasia and lactose intolerance. Scand. J. Gastroenterol. 29:36-54.
7. Savilahti, E., Launiala, K., and Kuitunen, P. 1983. Congenital lactase deficiency. Arch. Dis. Child. 58:246-252.
8. Cooper, B.T. 1986. Lactase deficiency and lactose malabsorption. Dig. Dis. 4:72-82.
9. Tamm, A. 1994. Management of lactose intolerance. Scand. J. Gastroenterol. 29:55-63.
10. Duluc, I. Boukamel, R., Mantei, N., Semenza, G., Raul, F. and Freund, J.N. 1991. Sequence of the precursor of intestinal lactase-phlorizin hydrolase from fetal rat. Gene 103:275-276.
11. Vilotte, J.L. and Soulier, S. 1992. Isolation and characterization of the mouse alpha-lactalbumin-encoding gene: interspecies comparison, tissue-and stage-specific expression. Gene 119:287-292.
12. Naim, H.Y., Sterchi, E.E., and Lentze, M.J. 1987. Biosynthesis and maturation of lactase-phlorizin hydrolase in the human small intestinal epithelial cells. Biochem. J. 241:427-434.
13. Büller, H.A., Montgomery, R.K., Sasak, W.V., and Grand, R.J. 1987. Biosynthesis, glycosylation and intracellular transport of intestinal lactase-phlorizin in rat. J. Biol. Chem. 262:17206-17211.
14. Naim, H.Y., Lacey, S.W., Sambrook, J.F., and Gething, M.J. 1991. Expression of a full-length cDNA coding for human intestinal lactase-phlorizin hydrolase reveals an uncleaved, enzymatically active, and transport-competent protein. J. Biol. Chem. 266:12313-12320.
15. Neele, A.M., Einerhand, A.W.C.,Dekker, J., Büller, H.A., Freund, J.N., Vernave, M. et al. 1995. Verification of the rat lactase-phlorizin hydrolase site by site-directed mutagenesis. Gastroenterology 109:1234-1240.
16. Jost, B., Duluc, I., Richardson, M., Lathe, R., and Freund, J.N. 1997. Functional diversity and interactions between the repeat domains of rat intestinal lactase. Biochem. J. 327:95-103.
17. Brand, J.C. and Holt, S. 1991. Relative effectiveness of milks with reduced amounts of lactose in alleviating milk intolerance. Am. J. Clin. Nutr. 54:148-151.
18. Vesa, T.H., Korpela, R.A., and Sahi, T. 1996. Tolerance to small amounts of lactose in lactose maldigesters. Am. J. Clin. Nutr. 64:197-201.
19. Faerman, A., Barash, I., Puzis, R., Nathan, M., Hurwitz, D.R., and Shani, M. 1995. Dramatic heterogeneity of transgene expression in the lactating mammary gland of lactating mice: a model system to study the synthetic activity of mammary epithelial cells. J. Histochem. Cytochem. 4:461-470.
20. Molenaar, A.J., Davis, S.R., and Wilkins, R.J. 1992. Expression of α-lactalbumin, αS1-casein and lactotransferrin genes is heterogeneous in sheep and cattle mammary tissue. J. Histochem. Cytochem. 40:611-618.
21. Sasaki, M., Eigel, W.N., and Keenan, T.W. 1978. Lactose and major milk proteins are present in secretory vesicle-rich fractions from lactating mammary gland. Proc. Natl. Acad. Sci. USA 75:5020-5024.
22. Stinnakre, M.G., Vilotte, J.L., Soulier, S., and Mercier, J.C. 1994. Creation and phenotypic analysis of alpha-lactalbumin-deficient mice. Proc. Natl. Acad. Sci. USA 91:6544-6548.
23. DiTullio, P., Cheng, S.H., Marshall, J., Gregory, R.J., Ebert, K.M., Meade, H.M. et al. 1992. Production of cystic fibrosis transmembrane conductance regulator in the milk of transgenic mice. Bio/Technology 10:74-77.
24. Drews R., Paleyanda R.K., Lee T.K., Chang R.R., Rehemtulla A., Kaufman R.J. et al. 1995. Proteolytic maturation of protein C upon engineering the mouse mammary gland to express furin. Proc. Natl. Acad. Sci. USA 92:10462-10466.
25. Hauri, H.P., Sterchi, E.E., Bienz, D., Fransen, J.A., and Marxer, A. 1985. Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells. J. Cell Biol. 101:838-851.
26. Panzer, P., Preuss, U., Joberty, G., and Naim, H.Y. 1998. Protein domains implicated in intracellular transport and sorting of lactase-phlorizin hydrolase. J. Biol. Chem. 273:13861-13869.
27. Patton, S. and Keenan, T.W. 1975. The milk fat globule membrane. Biochem. Biophys. Acta. 415:273-309.
28. Drohan, W.N., Zhang, D.W., Paleyanda, R.K., Chang, R., Wroble, M., Velander, W. et al. 1994. Inefficient processing of human protein C in the mouse mammary gland. Transgenic Res. 3:355-364.
29. Rusconi, S., Severne, Y., Georgiev, O., Galli, I., and Wieland, S. 1990. A novel expression assay to study transcriptional activators. Gene 89:211-221.
30. Vilotte, J.L., Soulier, S., Paly, J., L'Huillier, P.J., Djiane, J., and Mercier, J.C. 1996. Introduction of a proximal STAT5 binding site into the murine α-lactalbumin promoter eliminates constitutive activity and leads to prolactin dependency in CHO and HC11 cells. Animal Genet. 27:A99.
31. Lathe, R., Vilotte, J.L., and Clark, A.J. 1987. Plasmid and bacteriophage vectors for excision of intact inserts. Gene 57:193-201.
32. Vilotte, J.L., Soulier, S., Stinnakre, M.G., Massoud, M., and Mercier, J.C. 1989. Efficient tissue-specific expression of bovine alpha-lactalbumin in transgenic mice. Eur. J. Biochem. 186:43-48.
33. Simons, J.P., McClenaghan, M., and Clark, A.J. 1987. Alteration of the quality of milk by expression of sheep beta-lactoglobulin in transgenic mice. Nature 328:530-532.
34. Hansen, S.A. 1975. Thin-layer chromatographic method for identification of oligosaccharides in starch hydrolyzates. J. Chromatogr. 105:388-390.
35. Bordier, C. 1981. Phase separation of integral membrane proteins in Triton X-114 solution. J. Biol. Chem. 256:1604-1607.
36. Schmitz, J., Preiser, H., Maestracci, D., Ghosh, B.K., Cerda, J.J., and Crane, R.K. 1973. Purification of the human intestinal brush border membrane. Biochim. Biophys. Acta. 323:98-112.
37. Nsi-Emvo, E., Launay, J.F., and Raul, F. 1986. Improved purification of rat intestinal lactase. Gen. Physiol. Biophys. 5:53-59.
38. Duluc, I., Jost, B., and Freund, J.N. 1993. Multiple levels of control of the stage-and region-specific expression of rat intestinal lactase. J. Cell Biol. 123:1577-1586.
39. Duluc, I., Freund, J-N., Leberquier, C., and Kedinger, M. 1994. Fetal endoderm primarily holds the temporal and positional information required for mammalian intestinal development. J. Cell Biol. 126:211-221.
40. Lucas, A., Gibbs, J.A.H., and Baum, J.D. 1978. Creamatocrit: a simple clinical technique for estimating fat concentration and energy value of human milk. Br. Med. J. 1:1018-1020.
41. - activities during the progesterone-induced maturation of urodele amphibian oocytes. Dev. Biol. 120:481-493.
42. Koldovsky, O., Asp, N.G., and Dahlqvist, A. 1969. A method for the separate assay of "neutral" and "acid" beta-galactosidase in homogenates of rat small intestinal mucosa. Anal. Biochem. 27:409-418.