Biotinylation of class I MHC molecules abrogates recognition by W6/32 antibody

Tissue Antigens

Volumn 53, Issue 6, 1999, Pages 576-579

  Malik, P ^a   Baba, E ^a   Strominger, Jack L ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

Biotinylation; HLA; mAB; MHC; Recognition

Indexed keywords

BETA 2 MICROGLOBULIN; EPITOPE; HLA G ANTIGEN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; SUCCINIMIDE DERIVATIVE;

ARTICLE; BIOTINYLATION; FLUORESCENCE ACTIVATED CELL SORTER; MAJOR HISTOCOMPATIBILITY COMPLEX; PRIORITY JOURNAL;

ANTIBODIES, MONOCLONAL; ANTIGEN-ANTIBODY REACTIONS; BIOTIN; BIOTINYLATION; FLOW CYTOMETRY; HISTOCOMPATIBILITY ANTIGENS CLASS I; HUMANS;

EID: 0033041573     PISSN: 00012815     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-0039.1999.530609.x     Document Type: Article

References (12)

1. Altin JG, Pagler EB. A one-step procedure for biotinylation and chemical cross-linking of lymphocyte surface and intracellular membrane-associated molecules. Anal Biochem 1995: 224: 382-9.
2. Barnstable CJ, Bodmer WE Brown G et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - New tools for genetic analysis. Cell 1978: 14: 9-20.
3. Parham P, Barnstable CJ, Bodmer WF. Use of a monoclonal antibody (W6/32) in structural studies of HLA-A,B,C, antigens. J Immunol 1979: 123: 342-9.
4. Brodsky FM, Parham PJ. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J Immunol 1982: 128: 129-35.
5. Shimizu Y, DeMars R. Production of human cells expressing individual transferred HLA-A,-B,-C genes using an HLA-A,-B,-C null human cell line. J Immunol 1989: 142: 3320-8.
6. Brodsky FM, Bodmer WF, Parham P. Characterization of a monoclonal anti-beta 2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur J Immunol 1979: 9: 536-45.
7. Ellis SA, Taylor C, McMichael A, Recognition of HLA-B27 and related antigen by a monoclonal antibody. Hum Immunol 1982: 5: 49-59.
8. Benjamin R, Parham P. Guilt by association: HLA-B27 and ankylosing spondylitis. Immunol Today 1990: 11: 137-42.
9. Maziarz RT, Fraser J, Strominger JL, Burakoff SJ. The human HLA-specific monoclonal antibody W6/32 recognizes a discontinuous epitope within the alpha 2 domain of murine H-2Db. Immunogenetics 1986: 24: 206-8.
10. Jefferies WA, MacPherson GG. Expression of the W6/32 HLA epitope by cells of rat, mouse, human and other species: Critical dependence on the interaction of specific MHC heavy chains with human or bovine beta 2-microglobulin. Eur J Immunol 1987: 17: 1257-63.
11. Tanabe M, Sekimata M, Ferrone S, Takiguchi M. Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain. J Immunol 1992: 148: 3202-9.
12. Shields MJ, Ribaudo RK. Mapping of the monoclonal antibody W6/32: Sensitivity to the amino terminus of beta2-microglobulin. Tissue Antigens 1998: 51: 567-70.