Conformational probes of O-acetylserine sulfhydrylase: Fluorescence of tryptophans 50 and 161

Journal of Photochemistry and Photobiology B: Biology

Volumn 48, Issue 1, 1999, Pages 17-26

  Benci, Sara ^a   Bettati, Stefano ^a   Vaccari, Silvia ^a   Schianchi, Giulio ^a   Mozzarelli, Andrea ^a   Cook, Paul F ^b  

^a UNIVERSITY OF PARMA   (Italy)

^b UNIVERSITY OF OKLAHOMA   (United States)

Author keywords

Cysteine; Enzymes; Tryptophan fluorescence

Indexed keywords

3 CHLOROALANINE; ACETIC ACID; AMINE; APOENZYME; CYSTEINE; CYSTEINE SYNTHASE; IMINE; PYRIDOXAL 5 PHOSPHATE; SERINE; TRYPTOPHAN;

AMINO ACID SYNTHESIS; ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL;

EID: 0033038799     PISSN: 10111344     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1011-1344(99)00003-2     Document Type: Article

References (29)

1. P.F. Cook, R.T. Wedding, A reaction mechanism from steady state kinetic studies for O-acetylserine sulfhydrylase from Salmonella typhimurium LT-2, J. Biol Chem. 251 (1976) 2023-2029.
2. C.-H. Tai, S.R. Nalabolu, T.M. Jacobson, D.E. Minter, P.F. Cook, Kinetic mechanism of the A and B isozymes of O-acetylserine sulfhydrylase from Salmonella typhimurium LT-2 using natural and alternative reactants, Biochemistry 32 (1993) 6433-6442.
3. K.D. Schnackerz, C.-H. Tai, J.W. Simmons, T.M. Jacobson, G.S.J. Rao, P.F. Cook, Identification and spectral characterization of the external aldimine of the O-acetylserine sulfhydrylase reaction, Biochemistry 34 (1995) 12152-12160.
4. H.G. Floss, E. Schleicher, R. Potts, Stereochemistry of the formation of cysteine by O-acetylserine sulfhydrase, J. Biol. Chem. 251 (1976) 5478-5482.
5. 31PNMR spectra of O-acetylserine sulfhydrylase in the absence and presence of O-acetylserine, Biochemistry, 31 (1992) 2298-2303.
6. P. Burkhard, G.S.J. Rao, E. Hohenester, K.D. Schnackerz, P.F. Cook, J.N. Jansonius, The three dimensional structure of O-acetylserine sulfhydrylase from Salmonella typhimurium, J. Mol. Biol. 283 (1998) 121-133.
7. A. Mozzarelli, S. Bettati, A.M. Pucci, P. Burkhard, P.F. Cook, The catalytic competence of O-acetylserine sulfhydrylase in the crystal probed by polarized absorption microspectrophotometry, J. Mol. Biol. 283 (1998) 135-146.
8. G.B. Strambini, P. Cioni, P.P. Cook, Tryptophan luminescence as a probe of enzyme conformation along the O-acetylserine sulfhydrylase reaction pathway, Biochemistry 35 (1996) 8392-8400.
9. S. Vaccari, S. Benci, A. Peracchi, A. Mozzarelli, Time-resolved fluorescence of pyridoxal 5′-phosphate-containing enzymes: Tryptophan synthase and O-acetylserine sulfhydrylase, J. Fluorescence 7 (1997) 135S-137S.
10. S. Benci, S. Vaccari, A. Mozzarelli, P.F. Cook, Time-resolved fluorescence of O-acetylserine sulfhydrylase catalytic intermediates, Biochemistry 36 (1997) 15419-15427.
11. G.D. McClure, P.F. Cook, Product binding to the α-carboxyl subsite results in a conformational change at the active site of O-acetylserine sulfhydrylase: Evidence from fluorescence spectroscopy, Biochemistry 33 (1994) 1674-1683.
12. S. Hara, M.A. Payne, K.D. Schnackerz, P.F. Cook, A rapid purification procedure and computer-assisted sulfide ion electrode assay for O-acetylserine sulfhydrylase from Salmonella typhimurium, Prot. Expr. Purif. 1 (1990) 70-90.
13. K.D. Schnackerz, P.F. Cook, Resolution of pyridoxal 5′-phosphate from O-acetylserine sulfhydrylase from Salmonella typhimurium and reconstitution of apoenzyme with cofactor and cofactor analogues as a probe of a cofactor binding site, Arch. Biochem. Biophys. 324 (1995) 71-77.
14. M.A. Becker, N.M. Kredich, G.M. Tomkins, The purification and characterization of O-acetylserine sulfhydrylase from Salmonella typhimurium, J. Biol. Chem. 244 (1969) 2418-2427.
15. M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254.
16. E. Gratton, J.R. Alcala, G. Marriott, Rotations of tryptophan residues in proteins, Biochem. Soc. Trans. 14 (1986) 835-838.
17. J.M. Beechem, E. Gratton, Fluorescence spectroscopy data analysis environment: A second generation global analysis program, SPIE Proc. 909 (1988) 70-81.
18. J.R. Lakowicz (ed.), Topics in Fluorescence Spectroscopy, vol. 2, Plenum, New York, 1991, pp. 241-277.
19. G. Weber, Theory of differential phase fluorometry: Detection of anisotropic molecular rotations, J. Chem. Phys. 66 (1977) 4081-4091.
20. R.F. Steiner, Fluorescence anisotropy: Theory and applications, in: J.R. Lakowicz (Ed.), Topics in Fluorescence Spectroscopy, vol. 2, Plenum, New York, 1991, pp. 1-52.
21. R.M. Jameson, T.L. Hazlett, Time-resolved fluorescence in biology and biochemistry, in: T.G. Dewey (Ed.), Biophysical and Biochemical Aspects of Fluorescence Spectroscopy, Plenum, New York, 1991, pp. 105-133.
22. 6 and analogs, in: D. Dolphin, R. Poulson, O. Avramovic (Eds.), Coenzymes and Cofactors, Vitamin B6, Pyridoxal Phosphate, Wiley, New York, 1986, pp. 132-221.
23. M. Arrio-Dupont, Etude par fluorescence de bases de schiff du pyridoxal, comparaison avec la l-aspartate-aminotransferase, Photochem. Photobiol. 12 (1970) 297-315.
24. J.R. Lakowicz (ed.), Principles of Fluorescence Spectroscopy, Plenum, New York, 1983, pp. 155-194.
25. J.M. Beechem, L. Brand, Time resolved fluorescence of proteins, Ann. Rev. Biochem. 54 (1985) 43-71.
26. J.B.A. Ross, C.J. Schmidt, L. Brand, Time-resolved fluorescence of the two tryptophans in horse liver alcohol dehydrogenase, Biochemistry 20 (1981) 4369-4377.
27. R.G. Kallen, T. Korpela, A.E. Martell, Y. Matsushima, C.M. Metzler, D.E. Metzler, Y.V. Morozov, I.M. Ralston, F.A. Savin, Y.M. Torchinsky, H. Ueno, Chemical and spectroscopic properties of pyridoxal and piridoxamine phosphates, in: P. Christen, D.E. Metzler (Eds.), Transaminases, Wiley, New York, 1985, pp. 37-108.
28. S. Benci, S. Vaccari, A. Mozzarelli, P.F. Cook, Time-resolved fluorescence of O-acetylserine sulfhydrylase, Biochim. Biophys. Acta 1429 (1999) 317-330.
29. H. Fraunfelder, B. McMahon, Dynamics and function of proteins: The search for general concepts, Proc. Natl. Acad. Sci. USA 95 (1998) 4795-4797.