The gene encoding a periplasmic deoxyribonuclease from Aeromonas hydrophila

FEMS Microbiology Letters

Volumn 173, Issue 1, 1999, Pages 41-46

  Dodd, Helen N ^a   Pemberton, John M ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Aeromonas hydrophila; Deoxyribonucleases; Gene inactivation; Periplasmic enzyme

Indexed keywords

DEOXYRIBONUCLEASE; SIGNAL PEPTIDE;

AEROMONAS HYDROPHILA; ARTICLE; BACTERIUM CULTURE; CYTOCHEMISTRY; DNA TRANSFER; ELECTROPORATION; MOLECULAR CLONING; MUTANT; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

AEROMONAS HYDROPHILA; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ELECTROPORATION; ENDODEOXYRIBONUCLEASES; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; MUTATION; PERIPLASM; PLASMIDS; SEQUENCE ANALYSIS, DNA;

EID: 0033036288     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00054-3     Document Type: Article

References (23)

1. Gobius, K.S. and Pemberton, J.M. (1986) Use of plasmid pULB113 (RP4::mini-Mu) to construct a genomic map of Aeromonas hydrophila. Curr. Microbiol. 13, 111-115.
2. Dodd, H.N. and Pemberton, J.M. (1996) Cloning, sequencing and characterization of the nucH gene encoding an extracellular nuclease from Aeromonas hydrophila strain JMP636. J. Bacteriol. 178, 3926-3933.
3. Focareta, T. and Manning, P.A. (1991) Distinguishing between the extracellular DNases of Vibrio cholerae and development of a transformation system. Mol. Microbiol. 5, 2547-2555.
4. Reid, J.D., Stoufer, S.D. and Ogrydziak, D.M. (1982) Efficient transformation of Serratia marcescens with pBR322 plasmid DNA. Gene 17, 107-112.
5. Kawagishi, I., Okunishi, I., Homma, M. and Imae, Y. (1994) Removal of the periplasmic DNase before electroporation enhances the efficiency of transformation in the marine bacterium Vibrio alginolyticus. Microbiology 140, 2355-2361.
6. Focareta, T. and Manning, P.A. (1987) Extracellular proteins of Vibrio cholerae: molecular cloning, nucleotide sequence and characterization of the deoxyribonuclease (DNase) together with its periplasmic localization in Escherichia coli K-12. Gene 53, 31-40.
7. Moulard, M., Condemine, G. and Robert-Baudouy, J. (1993) Characterization of the nucM gene coding for a nuclease of the phytopathogenic bacteria Erwinia chrysanthemi. Mol. Microbiol. 8, 685-695.
8. Chang, M.C., Chang, S.Y., Chen, S.L. and Chuang, S.M. (1992) Cloning and expression in Escherichia coli of the gene encoding an extracellular deoxyribonuclease (DNase) from Aeromonas hydrophila. Gene 122, 175-180.
9. Jekel, M. and Wackernagel, W. (1995) The periplasmic endonuclease I of Escherichia coli has amino-acid sequence homology to the extracellular DNases of Vibrio cholerae and Aeromonas hydrophila. Gene 154, 55-59.
10. Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166, 557-580.
11. Penfold, R.J. and Pemberton, J.M. (1992) An improved suicide vector for construction of chromosomal insertion mutations in bacteria. Gene 116, 145-146.
12. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.
13. Quigley, N.B. and Reeves, P.R. (1987) Chloramphenicol resistance cloning vector based on pUC9. Plasmid 17, 54-57.
14. Penfold, R.J. (1994) Development of genetic tools and their use in the analysis of photosynthesis in Rhodobacter sphaeroides. PhD thesis, University of Queensland.
15. Pemberton, J.M. and Penfold, R.J. (1992) High frequency electroporation and maintenance of pUC- and pBR- based cloning vectors in Pseudomonas stutzeri. Curr. Microbiol. 25, 25-29.
16. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Thompson, J.D., Higgins, D.G. and Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.
18. von Heijne, G. (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14, 4683-4690.
19. Lindeberg, M. and Collmer, A. (1992) Analysis of eight out genes in a cluster required for pectic enzyme secretion by Erwinia chrysanthemi: sequence comparison with secretion genes from other Gram-negative bacteria. J. Bacteriol. 174, 7385-7397.
20. Hardie, K.R., Schulze, A., Parker, M.W. and Buckley, J.T. (1995) Vibrio spp. secrete proaerolysin as a folded dimer without the need for disulphide bond formation. Mol. Microbiol. 17, 1035-1044.
21. Reverchon, S. and Robert-Baudouy, J. (1985) Genetic transformation of the phytopathogenic bacteria, Erwinia chrysanthemi. Biochimie 67, 253-257.
22. Roberts, R.J. and Macelis, D. (1994) REBASE- restriction enzymes and methylases. Nucleic Acids Res. 22, 3628-3639.
23. Wackernagel, W. (1973) Genetic transformation in E. coli: the inhibitory role of the recBC DNase. Biochem. Biophys. Res. Commun. 51, 306-311.