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Poirier Y., Dennis D., Klomparens K., Nawrath C., Somerville C. Perspectives on the production of polyhydroxyalkanoates in plants. FEMS Microbiol Rev. 103:1992;237-246.
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0028577728
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Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high-levels of polymer accumulation
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Nawrath C., Poirier Y., Somerville C.R. Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high-levels of polymer accumulation. Proc Natl Acad Sci USA. 91:1994;12760-12764.
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A biodegradable rubber by crosslinking poly(hydroxyalkanoate) from Pseudomonas oleovorans
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Synthesis of medium-chain-length polyhydroxyalkanoates in Arabidopsis thaliana using intermediates of peroxisomal fatty acid β-oxidation
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This paper describes the synthesis of MCL-PHAs in plants through the expression of a PHA synthase in peroxisomes. The results demonstrate the possibility of synthesising a broad range of PHA in plants. Analysis of the monomer composition of the PHA produced also indicated that PHA can be used as a tool to analyse the β-oxidation pathway of fatty acids in plants.
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Mittendorf V., Robertson E.J., Leech R.M., Krüger N., Steinbüchel A., Poirier Y. Synthesis of medium-chain-length polyhydroxyalkanoates in Arabidopsis thaliana using intermediates of peroxisomal fatty acid β-oxidation. Proc Natl Acad Sci USA. 95:1998;13397-13402. This paper describes the synthesis of MCL-PHAs in plants through the expression of a PHA synthase in peroxisomes. The results demonstrate the possibility of synthesising a broad range of PHA in plants. Analysis of the monomer composition of the PHA produced also indicated that PHA can be used as a tool to analyse the β-oxidation pathway of fatty acids in plants.
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A. Steinbüchel. Weinheim: Wiley-VCH
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The efficient production of synthetic proteins made of repeated blocks of 34 amino acids, based on a spider dragline protein, is demonstrated in P. pastoris. Genes of up to 3000 codons could be expressed with no evidence of post-integrative DNA rearrangements or premature protein termination, as described for expression of similar proteins in E. coli.
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