The HET-CAM, a useful in vitro assay for assessing the eye irritation properties of cosmetic formulations and ingredients

Toxicology in Vitro

Volumn 13, Issue 2, 1999, Pages 375-384

  Steiling, W ^a   Bracher, M ^b   Courtellemont, P ^c   De Silva, O ^d  

^a HENKEL KGAA   (Germany)

^b Wella Cosmital SA   (Switzerland)

^c Christian Dior   (France)

^d L ORÉAL RECHERCHE   (France)

Author keywords

Draize test; Eye irritation; HET CAM; In vitro method; Ring trial; Validation

Indexed keywords

COSMETIC;

ANIMAL TISSUE; ARTICLE; CHEMICAL INDUSTRY; COSMETIC INDUSTRY; EYE IRRITATION; GOOD LABORATORY PRACTICE; MUCOSA; NONHUMAN; RISK ASSESSMENT; SCREENING TEST; VALIDATION PROCESS;

EID: 0033034890     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(98)00091-5     Document Type: Article

References (16)

1. Barratt M.D. QSARS for the eye irritation potential of neutral organic chemicals. ATLA. 24:1996;71.
2. Brantom P.G., Bruner L.H., Chamberlain M., De Silva O., Dupuis J., Earl L.K., Lovell D.P., Pape W.J.W., Uttley M., Bagley D.M., Baker F.W., Bracher M., Courtellemont P., Declercq L., Freeman S., Steiling W., Walker A.P. A summary report of the COLIPA International Validation Study on alternatives to the Draize rabbit eye irritation test. Toxicology in Vitro. 11:1997;141-179.
3. Chiou G. C. Y. (1992) Ophthalmic Toxicology. Target Organ Toxicology Series, Raven Press, New York.
4. Draize J. H. (1959) Dermal toxicity. In Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics, pp. 46-48. Association of Food & Drug Officials of the United States.
5. ECETOC (European Centre for Ecotoxicology and Toxicology of Chemicals) (1997) EC Classification of Eye Irritancy. Document No. 37.
6. European Communities (1991) Official Journal L180. In Adapting to technical progress for the Council Directive 67/548/EEC.
7. Gloxhuber Ch and Künstler K. (1992) Anionic surfactants, Surfactant Science Series, 2nd edn, Vol. 43. Marcel Dekker, New York.
8. Grant W. M. (1986) Toxicology of the Eye, 3rd edn. Charles C. Thomas Publisher, Springfield, USA.
9. Luepke N.P. Hen's egg chorioallantoic membrane test for irritation potential. Food and Chemical Toxicology. 23:1985;287-291.
10. Muir C.K., Flower C., Van Abbe N.J. A novel approach to the search for in vitro alternatives to in vivo eye irritancy testing. Toxicology Letters. 18:1983;1-5.
11. OECD Guidelines for testing of chemicals (1987) No. 405. Acute Eye Irritation/Corrosion.
12. Spielmann H., Liebsch M., Kalweit S., Moldenhauer F., Wirnsberger T., Holzhütter H.G., Schneider B., Glaser S., Gerner I., Pape W.J.W., Kreiling R., Krauser K., Miltenburger H.G., Steiling W., Luepke N.P., Müller N., Kreuzer H., Mürmann P., Spengler J., Bertram-Neis E., Siegemund B., Wiebel F.J. Results of a validation study in Germany on two in vitro alternatives to the Draize eye irritation test, the HET-CAM test and the 3T3 NRU cytotoxicity test. ATLA. 24:1996;741-858.
13. Steiling W. (1994) The Hen's Egg Test on the Chorioallantoic Membrane (HET-CAM). INVITOX protocol No. 96, INVITOX Data Bank, Nottingham, UK.
14. Steiling W., Kreutz J., Spanier M., Sterzel W. The HET-CAM, a reliable alternative to the Draize rabbit eye test. Naunyn Schmiedeberg's Archives of Pharmacology. Suppl. 353:1996;R117 (437).
15. Sterzel W., Bartnik F.G., Matthies W., Kästner W., Künstler K. Comparison of two in vitro and two in vivo methods for the measurement of irritancy. Toxicology in Vitro. 4:1990;698-701.
16. York M. and Steiling W. (1998) A critical review of the assessment of eye irritation potential using the Draize rabbit eye test. Journal of Applied Toxicology 18, 233-240.