Inhibition of NaCl-induced heat shock protein 72 expression renders MDCK cells susceptible to high urea concentrations

Pflugers Archiv European Journal of Physiology

Volumn 437, Issue 4, 1999, Pages 611-616

  Neuhofer, Wolfgang ^a   Müller, Eva ^a   Burger Kentischer, Anke ^a   Fraek, Maria Luisa ^a   Thurau, Klaus ^a   Beck, Franz X ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Antisense; Heat shock proteins; Hypertonic stress; MDCK cells; Transfection; Urea

Indexed keywords

HEAT SHOCK PROTEIN 72; HYPERTONIC SOLUTION; SODIUM CHLORIDE; UREA;

ANIMAL CELL; ARTICLE; CELL PROTECTION; CELL SURVIVAL; CELL VIABILITY; CONCENTRATION (PARAMETERS); GENETIC TRANSFECTION; KIDNEY CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0033033130     PISSN: 00316768     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004240050824     Document Type: Article

References (21)

1. Baler R, Welch WJ, Voellmy R (1992) Heat shock gene regulation by nascent polypeptides and denatured proteins: hsp70 as a potential autoregulatory factor. J Cell Biol 117:1151-1159
2. Beck FX, Schmolke M, Guder WG (1992) Osmolytes. Curr Opin Nephrol Hypertens 1:43-52
3. Burel C, Mezger V, Pinto M, Rallu M, Trigon S, Morange M (1992) Mammalian heat shock protein families. Expression and functions. Experientia 48:629-634
4. Burg MB, Kwon ED, Kültz D (1996) Osmotic regulation of gene expression. FASEB J 10:1598-1606
5. Cohen DM (1997) Mitogen-activated protein kinase cascades and the signaling of hyperosmotic stress to immediate early genes. Comp Biochem Physiol 117A:291-299
6. Cox RA, Kanagalingam K (1968) A spectrophotometric study of the denaturation of deoxyribonucleic acid in the presence of urea or formaldehyde and its relevance to the secondary structure of single-stranded polynucleotides. Biochem J 108:599-610
7. 32P-based hybridizations. Anal Biochem 210:235-244
8. Gabai VL, Meriin AB, Mosser DD, Caron AW, Rits S, Shifrin VI, Sherman MY (1997) HSP70 prevents activation of stress kinases. A novel pathway of cellular thermotolerance. J Biol Chem 272:18033-18037
9. Georgopoulos C, Welch WJ (1993) Role of the major heat shock proteins as molecular chaperones. Annu Rev Cell Biol 9:601-634
10. Jiang Y, Chen C, Li Z, Guo W, Gegner JA, Lin S, Han J (1996) Characterization of the structure and function of a new mitogen-activated protein kinase (p38). J Biol Chem 271: 17920-17926
11. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
12. Lee JC, Laydon JT, McDonnell PC, Gallagher TF, Kumar S, Green S, McNulty D, Blumenthal MJ, Heys JR, Landvatter SW (1994) A protein kinase involved in the regulation of inflammatory cytokine biosynthesis. Nature 372:739-746
13. Leroy C, Gobin R, Pisam M, Le Moal C, Ripoche P, Rousselet G (1997) Urea induces apoptosis in mIMCD3 cell line: rescue by NaCl (abstract). J Am Soc Nephrol 8:54A
14. Müller E, Neuhofer W, Ohno A, Rucker S, Thurau K, Beck FX (1996) Heat shock proteins HSP25, HSP60, HSP72, HSP73 in isoosmotic cortex and hyperosmotic medulla of rat kidney. Pflügers Arch 431:608-617
15. Neuhofer W, Müller E, Burger-Kentischer A, Beck FX (1998) Hypertonicity affects heat shock protein 27 and F-actin localization in Madin-Darby canine kidney cells. Kidney Int 54 [Suppl. 67]:S165-S167
16. Neuhofer W, Müller E, Burger-Kentischer A, Fraek ML, Thurau K, Beck FX (1998) Pretreatment with hypertonic NaCl protects MDCK cells against high urea concentrations. Pflügers Arch 435:407-414
17. Nowak TS Jr, Bond U, Schlesinger MJ (1990) Heat shock RNA levels in brain and other tissues after hyperthermia and transient ischemia. J Neurochem 54:451-458
18. Otto JJ (1993) Immunoblotting. Methods Cell Biol 37:105-117
19. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, vol. 3, 2nd edn. Cold Spring Harbor Laboratory Press, New York
20. Sheikh-Hamad D, Di Mari J, Suki WN, Safirstein R, Watts BA, Rouse D (1998) p38 kinase activity is essential for osmotic induction of mRNAs for HSP70 and transporter for organic solute betaine in Madin-Darby canine kidney cells. J Biol Chem 273:1832-1837
21. Somero GN, Yancey PH (1997) Osmolytes and cell-volume regulation: physical and evolutionary principles. In: Hoffman JF, Jamieson JD (eds) Handbook of physiology. Section 14: cell physiology. Oxford University Press, New York, pp 441-484