Redundant systems of phosphatidic acid biosynthesis via acylation of glycerol-3-phosphate or dihydroxyacetone phosphate in the yeast Saccharomyces cerevisiae

Journal of Bacteriology

Volumn 181, Issue 5, 1999, Pages 1458-1463

  Athenstaedt, Karin ^a   Weys, Sabine ^a   Paltauf, Fritz ^a   Daum, Günther ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

2 PROPANOL; GLYCEROPHOSPHATE; PHOSPHATIDIC ACID;

ACYLATION; ARTICLE; BACTERIAL METABOLISM; ENZYME ACTIVITY; GENE MUTATION; NONHUMAN; PHOSPHOLIPID METABOLISM; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0033033118     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.5.1458-1463.1999     Document Type: Article

References (29)

1. Athenstaedt, K., and G. Daum. 1997. Biosynthesis of phosphatidic acid in lipid particles and endoplasmic reticulum of Saccharomyces cerevisiae. J. Bacteriol. 179:7611-7616.
2. Bates, E. J., and E. D. Saggerson. 1979. A study of the glycerol phosphate acyltransferase and dihydroxyacetone phosphate acyltransferase activities in rat liver mitochondrial and microsomal fractions. Biochem. J. 182:751-762.
3. Bell, R. M., and R. A. Coleman. 1983. Enzymes of thacylglycerol formation in mammals, p. 87-111. In P. D. Boyer (ed.), The enzymes. Academic Press. New York, N.Y.
4. Christiansen, K. 1978. Triacylglycerol synthesis in lipid particles from baker's yeast (Saccharomyces cerevisiae). Biochim. Biophys. Acta 530:78-90.
5. 2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. J. Biol. Chem. 257:13028-13033.
6. Folch, J., M. Lees, and G. H. Sloane-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
7. Gaigg, B., R. Simbeni, C. Hrastnik, F. Paltauf, and G. Daum. 1995. Characterizalion of a microsomal subfraction associated with mitochondria of the yeast. Saccharomyces cerevisiae. Biochim. Biophys. Acta 1234:214-220.
8. Gurr, M. I. 1980. The biosynthesis of triacylglycerols, p. 205-248. In P. K. Stumpf (ed.), The biochemistry of plants, Vol. 4. Lipids: structure and function. Academic Press, Inc., New York, N.Y.
9. Haid, A., and M. Suissa. 1983. Immunochemical identification of membrane proteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Methods Enzymol. 96:112-205.
10. Hajra, A. K. 1995. Glycerolipid biosynthesis in peroxisomes (microbodies). Prog. Lipid Res. 34:343-364.
11. Hajra, A. K. 1997. Dihydroxyacetone phosphate acyltransferase. Biochim. Biophys. Acta 1348:27-34.
12. Johnston, J. M., and F. Paltauf. 1970. Lipid metabolism in inositol deficient yeast. Saccharomyces carlsbergensis. II. Incorporation of labeled precursors into lipids by whole cells and activities of some enzymes involved in lipid formation. Biochim. Biophys. Acta 218:431-440.
13. Jones, C. L., and A. K. Hajra. 1980. Properties of guinea pig liver peroxisomal dihydroxyacetone phosphate acyltransferase. J. Biol. Chem. 255:8289-8295.
14. Kelley, M. J., and G. M. Carman. 1987. Purification and characterization of CDP-diacylglycerol synthase from Saccharomyces cerevisiae. J. Biol. Chem. 262:14563-14570.
15. Kuchler, K., G. Daum, and F. Paltauf. 1986. Subcellular and submitochondrial localization of phospholipid-synthesizing enzymes in Saccharomyces cerevisiae. J. Bacteriol. 165:901-910.
16. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680-685.
17. Leber, R., E. Zinser, G. Zellnig, F. Paltauf, and G. Daum. 1994. Characterization of lipid particles of the yeast. Saccharomyces cerevisiae. Yeast 10: 1421-1428.
18. Leber, R., K. Landl, E. Zinser, H. Ahorn, A. Spök, S. D. Kohlwein, F. Turnowsky, and G. Daum. 1998. Dual localization of squalene epoxidase. Erglp, in yeast reflects a relationship between the endoplasmic reticulum and lipid particles. Mol. Biol. Cell 9:375-386.
19. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:255-275.
20. Morlock, K. R., J. J. McLaughlin, Y.-P. Lin, and G. M. Carman. 1991. Phosphatidate phosphatase from Saccharomyces cerevisiae. Isolation of 45-and 104-kDa forms of the enzyme that are differentially regulated by inositol. J. Biol. Chem. 266:3586-3593.
21. Nagiec, M. M., G. B. Wells, R. L. Lester, and R. C. Dickson. 1993. A suppressor gene that enables Saccharomyces cerevisiae to grow without making sphingolipids encodes a protein that resembles an Escherichia coli fatty acyltransferase. J. Biol. Chem. 268:22156-22163.
22. Racenis, P. V., J. L. Lai, A. K. Das, P. C. Mullick, A. K. Hajra, and M. L. Greenberg. 1992. The acyl dihydroxyacetone phosphate pathway enzymes for glycerolipid biosynthesis are present in the yeast Saccharomyces cerevisiae. J. Bacteriol. 174:5702-5710.
23. Schlossman, D. M., and R. M. Bell. 1978. Glycerolipid biosynthesis in Saccharomyces cerevisiae: sn-glycerol-3-phosphate and dihydroxyacetone phosphate acyltransferase activities. J. Bacteriol. 133:1368-1376.
24. Schneiter, R. Personal communication.
25. Sherman, F. 1991. Getting started with yeast. Methods Enzymol. 194:3-21.
26. Tillman, T. S., and R. M. Bell. 1986. Mutants of Saccharomyces cerevisiae defective in sn-glycerol-3-phosphate acyltransferase. J. Biol. Chem. 261:9144-9149.
27. Van den Bosch, H. 1974. Phosphoglyceride metabolism. Annu. Rev. Biochem. 43:243-277.
28. Zinser, E., and G. Daum. 1995. Isolation and biochemical characterisation of organelles from the yeast. Saccharomyces cerevisiae. Yeast 11:493-536.
29. Zinser, E., C. D. M. Sperka-Gottlieb, E.-V. Fasch, S. D. Kohlwein, F. Paltauf, and G. Daum. 1991. Phospholipid synthesis and lipid composition of subcellular membranes in the unicellular eucaryote Saccharomyces cerevisiae. J. Bacteriol. 173:2026-2034.