Induction of antisense Pax-3 expression leads to the rapid morphological differentiation of neuronal cells and an altered response to the mitogenic growth factor bFGF

Journal of Cell Science

Volumn 112, Issue 2, 1999, Pages 253-261

  Reeves, F C ^a   Burdge, G C ^a   Fredericks, W J ^b   Rauscher III, F J ^b   Lillycrop, K A ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b WISTAR INSTITUTE   (United States)

Author keywords

Antisense; Differentiation; FGFR; Neuronal cell; Pax 3

Indexed keywords

BASIC FIBROBLAST GROWTH FACTOR; COMPLEMENTARY RNA;

ARTICLE; CELL LINE; CELL PROLIFERATION; EXPRESSION VECTOR; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; MOUSE; NERVE CELL DIFFERENTIATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; CELL DIFFERENTIATION; CELL DIVISION; CELL LINE; DNA-BINDING PROTEINS; DOWN-REGULATION; EYE PROTEINS; FIBROBLAST GROWTH FACTOR 2; HOMEODOMAIN PROTEINS; HUMANS; MICE; MUTATION; NEURONS; PAIRED BOX TRANSCRIPTION FACTORS; REPRESSOR PROTEINS; RNA, ANTISENSE; TRANSCRIPTION FACTORS; TRANSFECTION;

EID: 0033032947     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Bernasconi, M., Rempis, A., Fredericks, W. J., Rauscher III, F. J. and Schaffer, B. W. (1996). Induction of apoptosis in rhabdomyosarcoma cells through downregulation of PAX proteins. Proc. Natl. Acad. Sci. USA 93, 13164-13169.
2. Bopp, D., Burri, M., Baumgartner, S., Frigerio, G. and Noll, M. (1986) Conservation of a large protein domain in the segmentation gene paired and in functionally related genes of Drosophila. Cell 47, 1033-1040.
3. Bober, E., Franz, T., Arnold, H.-H., Gruss, P. and Tremblay, P. (1994). Pax-3 is required for the development of limb muscles: a possible role for the migration of dermomyotomal muscle progenitor cells. Development 120, 603-612.
4. Burri, M., Tromvoukis, Y., Bopp, D., Frigerio, G. and Noll, M. (1989) Conservation of the paired domain in metazoans and its structure in three isolated human genes. EMBO J. 8, 1183-1190.
5. 2H), a mutation affecting the development of the mouse neural tube, shows a deletion within the paired homeodomain of Pax-3 Cell 67, 767-774.
6. Epstein, J. A., Lam, P., Jepeal, L., Maas, R. L. and Shapiro, D. N. (1995) Pax-3 inhibits myogenic differentiation of cultured myoblast cells. 7. Biol. Chem. 270, 11719-11722.
7. Evans, J. and Lillycrop, K. A. (1996). Serum growth factor regulation of the paired box transcription factor Pax-3 in neuronal cells. Neurosci. Lett. 220, 125-128.
8. Feinberg, A. P. and Vogelstein, B. (1984). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6-13.
9. Foy, C., Newton, V., Wellesley, D., Harris, R. and Read, A. P. (1990) Assignment of the locus for Waardenburg syndrome type I to human chromosome 2q37 and possible homology to the splotch mouse. Am. J. Hum. Genet. 46, 1017-1023.
10. d) alleles: differential phenotypic effects on neural crest and limb musculature. Anat. Embryol. 187, 371-377.
11. Fredericks, W. J., Galili, N., Mukhopadhyay, S., Rovera, G., Bennicelli, J., Barr, F. G and Rauscher III, F. J. (1995). The PAX3-FKHR fusion protein created by the t(2; 13) translocation in alveolar rhabdomyosarcoma is a more potent transcriptional activator than PAX3. Mol. Cell Biol. 15, 1522-1535.
12. Gorman, C. (1986). High efficiency gene transfer into mammalian cells. In DN4 Cloning Volume II - A practical approach (ed. D. M. Glover), pp. 143-190. IRL Press, Oxford.
13. Goulding, M. D., Chalepakis, G., Deutsch, U., Erselius, J. and Gruss, P. (1992). A novel murine DNA binding protein expressed during early neurogenesis. EMBO J. 10, 1135-1147.
14. Hoth, C. F., Milunsky, A., Lipsky, N., Sheffer, R., Clarren, S. K., Baldwin, C. T. (1993). Mutations in the paired domain of the human PAX-3 gene cause Klien-Waardenhurg syndrome (WSIII) as well as Waaurdenburg syndrome type I (WSI). Am. J. Hum. Genet. 52, 455-462.
15. Itoh, N., Mima, T. and Mikawa, T. (1996). Loss of fibroblast growth factor receptors is necessary for terminal differentiation of embryonic limb muscle. Development 122, 291-300.
16. Kioussi, C., Gross, M. K. and Gruss, P. (1995). Pax-3: A paired domain gene as a regulator in PNS myelination. Neuron 15, 553-562.
17. Lakin, N. D., Morris, P. M., Theil, T., Sato, S. N., Moroy, T., Wilson, M. C. and Latchman, D. S. (1995). Regulation of neurite outgrowth and SNAP-25 gene expression by the Brn-3a transcription factor. J. Biol. Chem. 270, 15858-15863.
18. Orr-Urtreyer, A., Givol, D., Yayon, A., Yarden, Y and Lonai, P. (1991). Developmental expression of two murine fibroblast growth factor receptors, flg and bek. Development 113, 1419-1434.
19. Reeves, F. C., Fredericks, W. J., Rauscher III, F. J. and Lillycrop, K. A. (1998). The DNA binding activity of the paired box transcription factor Pax-3 is rapidly downregulated during neuronal cell differentiation. FEBS Lett. 422, 118-122.
20. Russell, W. L. (1947). Splotch, a new mutation in the house mouse Mus musculus. Genetics 32, 107.
21. Salomoni, P., Perrotti, D., Martinez, R., Franceshi, C. and Calabretta, B. (1997). Resistance to apoptosis in CTLL-2T cells constitutively expressing c-Myb is associated with induction of BCL-2 expression and Myb dependent regulation of bcl-2 promoter activity. Proc. Natl. Acad. Sci. USA 94, 3296-3301.
22. Taassabheji, M., Read, A. P., Newton, V. E., Patton, M. and Gruss, P. (1993). Mutations in the PAX-3 gene causing Waardenburg syndrome type I and type 2. Nature Genet. 3, 26-30.
23. Thompson, M. A. and Ramsay, R. G. (1995). Myb: an old oncoprotein with new roles. BioEssays 17, 341-350.
24. Treisman, J., Harris, E. and Desplan, C. (1991). The paired domain encodes a second DNA binding domain in the paired homeodomain protein. Genes Dev. 5, 594-604.
25. Walter, C., Guenet, J.-L, Simon, D., Deutsch, U. and Josles, B. (1991). Pax: a murine multigene family of paired box containing genes. Genomics 11, 424-434.