Live confocal microscopy of oligonucleotide uptake by keratinocytes in human skin grafts on nude mice

Journal of Investigative Dermatology

Volumn 112, Issue 6, 1999, Pages 887-892

  White, Paul J ^a   Fogarty, Rhys D ^a   Liepe, Ingrid J ^a   Delaney, Peter M ^b   Werther, George A ^a   Wraight, Christopher J ^a  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

^b NONE

Author keywords

Anti sense; DNA; Localization

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; FLUORESCEIN ISOTHIOCYANATE; LIPOSOME; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE;

ANIMAL TISSUE; ARTICLE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FEMALE; GENE THERAPY; HUMAN; HUMAN TISSUE; INTRADERMAL DRUG ADMINISTRATION; KERATINOCYTE; MOUSE; NONHUMAN; NUDE MOUSE; PRIORITY JOURNAL; SKIN GRAFT; SKIN PENETRATION; STRATUM CORNEUM; TOPICAL DRUG ADMINISTRATION;

EID: 0033028953     PISSN: 0022202X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1523-1747.1999.00593.x     Document Type: Article

References (12)

1. Alexander MY, Akhurst RJ: Liposome-mediated gene transfer and expression via the skin. Hum Mol Genet 12:2279-2285, 1995
2. Beltinger C, Saragovi HU, Smith RM, et al: Binding, uptake and intracellular trafficking of phosphorothioate-modified oligodeoxynucleotides. J Clin Invest 95:1814-1823, 1995
3. Bennett RM: As nature intended? The uptake of DNA and ODNs by eukaryotic cells. Antisense Res Dev 3:235-241, 1993
4. Flanagan WM, Su LL, Wagner RW: Elucidation of gene function using C-5 propyne antisense oligonucleotides. Nature Biotech 14:1139-1145, 1996
5. Giachetti C, Chin DJ: Increased oligonucleotide permeability in keratinocytes of artificial skin correlates with differentiation and altered membrane function. J Invest Dermatol 107:256-262, 1996
6. Khavari PA, Krueger GG: Cutaneous gene therapy. Adv Clin Res 15:27-35, 1997
7. Nanney LB, Magid M, Stoscheck CM, King LE: Altered 1251-epidermal growth factor binding and receptor distribution in psoriasis. J Invest Dermatol 86:260-265, 1986
8. Nestle OF, Mitra RS, Bennett CF, Chan H, Nickoloff B: Cationic lipid is not required lor selective inhibitory activity of ICAM-1 phosphorothioate antisense oligonucleotides in keratinocytes. J Invest Dermatol 103:569-575, 1994
9. Noonberg SB, Garavoy MR, Hunt CA: Characteristics of oligonucleotide uptake in human keratinocyte cultures. J Invest Dermatol 101:727-731, 1993
10. Setoguchi Y, Jaffe HA, Danel C, Crystal RG: Ex vivo and in vivo gene transfer to the skin using replicant-deficient recombinant adenovirus vectors. J Invest Dermatol 102:415-421, 1994
11. Stein CA, Tonkinson JL, Zhang LM, Yakubov L, Gervasoni J, Taub R, Rotenberg SA: Dynamics of the internalization of phosphodiester oligodeoxynucleotides in HL60 cells. Biochemistry 32:4855-4861, 1993
12. While PJ, Fogarty RD, McKean SC, Venables DJ, Werther GA, Wraight CJ: Oligonucleotide uptake in cultured keratinocytes: Influence of confluence, cationic liposomes, and keratinocyte cell type. J Invest Dermatol 112: in press, 1999