A method for identifying differentially expressed genes in rare populations of primary human hematopoietic cells

Leukemia

Volumn 13, Issue 2, 1999, Pages 295-297

  Hung, H L ^a,b,e   Song, F ^c,d   Gewirtz, A ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b CHILDREN S HOSPITAL OF PHILADELPHIA   (United States)

^c Center for Language and Brain   (United States)

^d MOUNT SINAI MEDICAL CENTER   (United States)

^e UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Differential display; Hematopoiesis; PCR

Indexed keywords

COMPLEMENTARY DNA; RNA;

ARTICLE; CELL POPULATION; CLONE; GENE EXPRESSION; GENE ISOLATION; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; NORTHERN BLOTTING; PRIORITY JOURNAL; SCREENING;

EID: 0033027925     PISSN: 08876924     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.leu.2401274     Document Type: Article

References (18)

1. Liang P, Pardee AB. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 1992; 257: 967-971.
2. Liang P, Bauer D, Averboukh L, Warthoe P, Rohrwild M, Muller H, Strauss M, Pardee AB. Analysis of altered gene expression by differential display. Meth Enzymol 1995; 254: 304-321.
3. Sargent T. Isolation of differentially expressed genes. Meth Enzymol 1987; 152: 423-432.
4. Hubank M, Schatz D. Identifying differences in mRNA expression by representational difference analysis of cDNA. Nucleic Acids Res 1994; 22: 5640-5648.
5. Wan JS, Sharp SJ, Poirier GM, Wagaman PC, Chambers J, Pyati J, Hom Y-L, Galindo JE, Huvar A, Peterson PA, Jackson MR, Erlander MG. Cloning differentially expressed mRNAs. Nat Biotech 1996; 14: 1685-1691.
6. Zhang H, Zhang R, Liang P. Differential screening of gene expression difference enriched by differential display. Nucleic Acid Res 1996; 24: 2454-2455.
7. Liu C, Raghothama KG. Practical methods for cloning cDNAs generated in an mRNA differential display. BioTechniques 1996; 20: 576-579.
8. Gewirtz AM, Xu WY, Mangan KF. Role of natural killer cells, in comparison with T lymphocytes and monocytes in the regulation of normal human megakaryocytopoiesis in vitro. J Immunol 1987; 139; 2915-2924.
9. Holmes K, Fowlkes BJ, Schmid I, Giorgi JV. Immunofluorescence and cell sorting. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W (eds). Current Protocols in Immunology. John Wiley and Sons: New York, 1994, pp 5.3.1-5.3.23.
10. Chomczynski P, Sacchi N. Anal Biochem 1987; 162: 156-159.
11. Sambrook J, Fritsch RF, Maniatis T. Molecular Cloning. Cold Spring Harbor Laboratories: Cold Spring Harbor, NY, 1989.
12. Chenchik A, Diatchenko L, Zhu Y, Siebert P. A novel technology for synthesizing full length cDNA from nanogram quantities of total RNA. J NIH Res 1996; 8: 60.
13. Peliska JA, Benkovic SJ. Fidelity of in vitro DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase. Biochemistry 1994; 33: 3890-3895.
14. Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983; 132: 6-13.
15. Ross R, Kumpf K, Reske-Kunz AB. PCR-amplified cDNA probes for verification of differentially expressed genes. BioTechniques 1997; 22: 894-897.
16. Bryan TM, Sperger JM, Chapman KB, Cech TR. Telomerase reverse transcriptase genes identified in Tetrahymena thermophila and Oxytricha trifallax. Proc Natl Acad Sci USA 1998; 95: 8479-8484.
17. Furumura M, Sakai C, Potterf SB, Vieira WD, Barsh GS, Hearing VJ. Characterization of genes modulated during pheomelanogenesis using differential display. Proc Natl Acad Sci USA 1998; 95: 7374-7378.
18. Mao M, Fu G, Wu J-S, Zhang Q-H, Zhou J, Kan L-X, Huang Q-H, He K-L, Gu B-W, Han Z-G, Shen Y, Gu J, Yu Y-P, Yu S-H, Wang Y-X, Chen S-J, Chen Z. Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning. Proc Natl Acad Sci USA 1998; 95: 8175-8180.