Structural characterization of acetobacter diazotropicus levansucrase by matrix-assisted laser desorption/ionization mass spectrometry: Identification of an N-terminal blocking group and a free-thiol cysteine residue

Journal of Mass Spectrometry

Volumn 34, Issue 3, 1999, Pages 169-174

  Betancourt, Lazaro ^a   Takao, Toshifumi ^a   Hernandez, Lazaro ^b   Padron, Gabriel ^b   Shimonishi, Yasutsugu ^a  

^a OSAKA UNIVERSITY   (Japan)

^b CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Free cysteine; Levansucrase; Matrix assisted laser desorption ionization time of flight post source decay; N terminal blocking; Primary structure

Indexed keywords

ASPARAGINE; ASPARTIC ACID; BACTERIAL ENZYME; CYSTEINE; LEVANSUCRASE; MUTANT PROTEIN; THIOL; THIOL REAGENT;

ACETOBACTER; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; DISULFIDE BOND; ENZYME STRUCTURE; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

ACETOBACTER; AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; DISULFIDES; HEXOSYLTRANSFERASES; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0033027216     PISSN: 10765174     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-9888(199903)34:3<169::AID-JMS780>3.0.CO;2-4     Document Type: Article

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