Method for the preparation of bispecific F(ab')2μ fragments from mouse monoclonal antibodies of the immunoglobulin M class and characterization of the fragments

Journal of Immunological Methods

Volumn 224, Issue 1-2, 1999, Pages 43-50

  Morimoto, Koichi ^a   Inouye, Kuniyo ^b  

^a TOSOH CORPORATION   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

Biosensor; Bispecific antibody; ELBA; F(ab') 2 fragments; IgM; Monoclonal antibody

Indexed keywords

CARCINOEMBRYONIC ANTIGEN; IMMUNOGLOBULIN F(AB')2 FRAGMENT; IMMUNOGLOBULIN M; MONOCLONAL ANTIBODY;

ANALYTIC METHOD; ANIMAL CELL; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ARTICLE; BIOSENSOR; ENZYME IMMUNOASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; IMMUNOREACTIVITY; MOLECULAR INTERACTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SOLUBILITY;

ANIMALIA;

EID: 0033025678     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00007-1     Document Type: Article

References (21)

1. Abe N., Inouye K. Purification of monoclonal antibodies with light-chain heterogeneity produced by mouse hybridomas raised with NS-1 myelomas. Application of hydrophobic interaction high-performance liquid chromatography. J. Biochem. Biophys. Methods. 27:1993;215.
2. Brennan M., Davison P.F., Paulus H. Preparation of bispecific antibodies by chemical recombination of monoclonal immunoglobulin G1 fragments. Science. 229:1985;81.
3. Glennie M.J., McBride H.M., Worth A.D., Stevenson G.T. Preparation and performance of bispecific F(ab′γ)2 antibody containing thioether-linked Fab′γ fragments. J. Immunol. 139:1987;2367.
4. Hartmann F., Renner C., Jung W., Deisting C., Juwana M., Eichentopf B., Kloft M., Pfreundschuh M. Treatment of refractory Hodgkin's disease with an anti-CD16/CD30 bispecific antibody. Blood. 89:1997;2042.
5. Holliger P., Prospero T., Winter G. 'Diabodies': small bivalent and bispecific antibody fragments. Proc. Natl. Acad. Sci. USA. 90:1993;6444.
6. Horenstein A.L., Poiesi C., DeMonte L., Camagna M., Mariani M., Albertini A., Malavasi F. Real-time kinetic analysis applied to the production of bispecific monoclonal antibodies for radioimmunodetection of cancer. Int. J. Clin. Lab. Res. 23:1993;199.
7. Inouye K. Bispecific-Ab-based immunoassay of thyroid-stimulating hormone. Cancer Immunol. Immunother. 45:1997;159.
8. Inouye K., Morimoto K. Single-step purification of F(ab′)2μ fragments of mouse monoclonal antibodies (immunoglobulins M) by hydrophobic interaction high-performance liquid chromatography using TSKgel Ether-5PW. J. Biochem. Biophys. Methods. 26:1993;27.
9. Inouye K., Morimoto K. Preparation of F(ab′)2μ fragments from rat IgM monoclonal antibodies and their application to the enzyme immunoassay of mouse interleukin-6. J. Immunol. Methods. 171:1994;239.
10. Inouye K., Kurokawa M., Nishikawa S., Tsukada M. Use of silk fibroin as a substratum for cultivation of animal cells. J. Biochem. Biophys. Methods. 37:1998;159.
11. Karlsson R., Fägerstam L., Nilshans H., Persson B. Analysis of active antibody concentration. Separation of affinity and concentration parameters. J. Immunol. Methods. 166:1993;75.
12. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680.
13. Merchant A.M., Zhu Z., Yuan J.Q., Goddard A., Adams C.W., Presta L.G., Carter P. An efficient route to human bispecific IgG. Nature Biotechnol. 16:1998;677.
14. Milstein C., Cuello A.C. Hybrid hybridomas and their use in immunohistochemistry. Nature. 305:1983;537.
15. Morimoto K., Inouye K. Single-step purification of F(ab′)2 fragments of mouse monoclonal antibodies (immunoglobulins G1) by hydrophobic interaction high-performance liquid chromatography using TSKgel Phenyl-5PW. J. Biochem. Biophys. Methods. 24:1992;107.
16. Morimoto K., Inouye K. A sensitive enzyme immunoassay of human thyroid-stimulating hormone (TSH) using bispecific F(ab′)2 fragments recognizing polymerized alkaline phosphatase and TSH. J. Immunol. Methods. 205:1997;81.
17. Morimoto K., Inouye K. Flow cytometric analysis of sialyl Lewis A antigen on human cancer cells by using F(ab′)2μ fragments prepared from a mouse IgM monoclonal antibody. Cytotechnology. 24:1997;219.
18. Ohnaka S., Soga E., Inouye K. Deletion of the κ genes from the mouse hybridoma established with NS-1 myelomas. Cytotechnology. 24:1997;213.
19. Ohta S., Tsukamoto H., Watanabe K., Makino K., Kuge S., Hanai N., Habu S., Nishimura T. Tumor-associated glycoantigen, sialyl Lewis A as a target for bispecific antibody-directed adoptive tumor immunotherapy. Immunol. Lett. 44:1985;35.
20. Suresh M.R., Cuello A.C., Milstein C. Advantages of bispecific hybridomas in one-step immunocytochemistry and immunoassays. Proc. Natl. Acad. Sci. USA. 83:1986;7989.
21. Takahashi M., Fuller S.A. Production of murine hybrid-hybridomas secreting bispecific monoclonal antibodies for use in urease based immunoassays. Clin. Chem. 34:1988;1693.