Development and in vitro characterization of human recombinant thyrotropin

Thyroid

Volumn 9, Issue 5, 1999, Pages 447-450

  Weintraub, Bruce D ^a,b   Szkudlinski, Mariusz W ^a,b  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b INSTITUTE OF HUMAN VIROLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; CHORIONIC GONADOTROPIN; FOLLITROPIN; GLYCOPROTEIN; GROWTH FACTOR; HUMAN GROWTH HORMONE; LYSINE; OLIGOSACCHARIDE; THYROTROPIN; TRANSFORMING GROWTH FACTOR;

ALPHA CHAIN; AMINO ACID SEQUENCE; BETA CHAIN; BINDING AFFINITY; CHIMERA; CONFERENCE PAPER; GLYCOSYLATION; MOLECULAR CLONING; MUTAGENESIS; PRIORITY JOURNAL; RECEPTOR BINDING; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; SYMPOSIUM; THYROID CANCER; THYROTROPIN BLOOD LEVEL;

EID: 0033024812     PISSN: 10507256     EISSN: None     Source Type: Journal    
DOI: 10.1089/thy.1999.9.447     Document Type: Conference Paper

References (12)

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4. Thotakura NR, Desai RK, Bates LG, Cole ES, Pratt BM, Weintraub BD 1991 Biological activity and metabolic clearance of a recombinant human thyrotropin produced in Chinese hamster ovary cells. Endocrinology 128:341-348.
5. Szkudlinski MW, Thotakura NR, Bucci I, Joshi LR, Tsai A, East-Palmer J, Shiloach J, Weintraub BD 1993 Purification and characterization of recombinant human thyrotropin (TSH) isoforms produced by Chinese hamster ovary cells: The role of sialylation and sulfation in TSH bioactivity. Endocrinology 133:1490-1503.
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7. Ladenson PW, Braverman LE, Mazzaferri EL, Robbins J, Cooper DS, Garber JR, Wondisford FE, Davies TF, DeGroot LJ, Daniels GH, Ross DS, Hay ID, Levis S, Bruckner-Davis F, Reynolds JC, Becker DV, Cavalieri RR, Maxon HR, McEllin K, Moscicki R, Weintraub BD 1997 Comparison of recombinant human thyrotropin administration to thyroid hormone withdrawal for radioactive iodine scanning in patients with thyroid carcinoma. N Engl J Med 337:888-896.
8. Joshi L, Murata Y, Wondisford FE, Szkudlinski MW, Desai R, Weintraub BD 1995 Recombinant TSH containing a β-subunit chimera with the human CG-b carboxy-terminus is biologically active, with a prolonged half-life: Role of carbohydrate in bioactivity and metabolic clearance. Endocrinology 136:3839-3848.
9. Szkudlinski MW, Teh NG, Grossmann M, Tropea JE, Weintraub BD 1996 Engineering human glycoprotein hormone superactive analogues. Nat Biotechnol 14:1257-1263.
10. Grossmann M, Weintraub BD, Szkudlinski MW 1998 A novel strategy for rational design of protein hormone superagonists. Nat Biotechnol 16:871-876.
11. Leitolf H, Grossmann M, Weintraub BD, Szkudlinski MW 1998 Introduction of multiple lysine residues into the common (alpha) L3 loop of glycoprotein hormones generates human thyrotropin superagonists and demonstrates cooperative effects with the thyrotropin (beta)L3 loop. 71st Annual Meeting of the American Thyroid Association, Portland, OR, September 16-20, 1998, (Abstract #221).
12. Szkudlinski MW, Dong W, Leitolf H, Knee R, Bhat MK, Weintraub BD 1998 Site-specific increase of human TSH bioactivity resulting from neutralization of acidic and introduction of basic residues in the TSH(beta)-subunit. 71st Annual Meeting of the American Thyroid Association, Portland, OR, September 16-20, 1998, (Abstract #196).