Transforming growth factor-β2 mRNA level in unloaded bone analyzed by quantitative in situ hybridization

Calcified Tissue International

Volumn 64, Issue 6, 1999, Pages 522-526

  Zhang, R ^a   Supowit, S C ^b   Hou, X ^c   Simmons, D J ^c  

^a PFIZER INC   (United States)

^b University of Texas Medical Branch School of Medicine   (United States)

^c UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

Author keywords

Osteocytes; Osteopenia; Periosteum; TGF 2

Indexed keywords

MESSENGER RNA; TRANSFORMING GROWTH FACTOR BETA2;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BONE STRESS; CONTROLLED STUDY; GENE EXPRESSION; GROWTH PLATE; HYPOKINESIA; MALE; NONHUMAN; OSTEOBLAST; OSTEOCYTE; OSTEOMETRY; OSTEOPENIA; PATHOPHYSIOLOGY; PERIOSTEUM; PRIORITY JOURNAL; RAT; RNA ANALYSIS; TISSUE DISTRIBUTION;

ANIMALS; BODY WEIGHT; BONE DISEASES, METABOLIC; FEMUR; HINDLIMB SUSPENSION; HUMERUS; IMAGE PROCESSING, COMPUTER-ASSISTED; IN SITU HYBRIDIZATION; OSTEOBLASTS; PERIOSTEUM; RATS; RATS, WISTAR; RNA, MESSENGER; SIGNAL TRANSDUCTION; TRANSFORMING GROWTH FACTOR BETA;

EID: 0033022466     PISSN: 0171967X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002239900643     Document Type: Article

References (23)

1. Wronski TJ, Morey ER (1982) Skeletal abnormalities in rats induced by simulated weightlessness. Metab Bone Dis Rel Res 4:69-75
2. Turner RT, Robyn JD, Duvall P, Morey ER, Baylink DJ, Spector M (1981) Evidence for arrested bone formation during spaceflight. Physiologist 24:S97-S98
3. Vico L, Chappard D, Alexandre C, Palle S, Minaire P, Riffat G, Morukov B, Rakhmanov S (1987) Effects of a 120-day period of bed rest on bone mass and bone cell activities in man: attempts at countermeasure. Bone Miner 2:383-394
4. Morey ER, Baylink DJ (1978) Inhibition of bone formation during spaceflight. Science 201:1138-1141
5. Zhang R, Supowit SC, Klein GL, Lu Z, Christensen MD, Lozano R, Simmons DJ (1995) Rat tail suspension reduces messenger RNA level for growth factors and osteopontin and decreases the osteoblastic differentiation of bone marrow stromal cells. J Bone Miner Res 10:415-423
6. 2 prevents the impaired chondrocyte proliferation induced by unloading in growth plates of young rats. Life Sci 61:2397-2406
7. Carmeliet G, Nys G, Stockmans I, Bouillon R (1998) Gene expression related to the differentiation of osteoblastic cells is altered by microgravity. Bone 22 (suppl)5:139S-143S
8. Sporn MB, Roberts AB (1989) Transforming growth factor-β. Multiple actions and potential clinical applications. JAMA 262:938-941
9. 1, and bone morphogenetic protein 4 on gene expression and differentiated function of preosteoblasts. J Cell Physiol 155: 112-119
10. Centrella M, McCarthy TL, Canalis E (1988) Parathyroid hormone modulates transforming growth factor β activity and binding in osteoblast-enriched cell cultures from fetal rat parietal bone. Proc Natl Acad Sci USA 85:5889-5893
11. Hock JM, Canalis E, Centrella M (1990) Transforming growth factor beta stimulates bone matrix apposition and bone cell replication in cultured fetal rat calvarias. Endocrinology 126: 421-426
12. Noda M (1989) Transcriptional regulation of osteocalcin production by transforming growth factor-β in rat osteoblast-like cells. Endocrinology 124:612-617
13. Joyce ME, Roberts AB, Sporn MB, Bolander ME (1990) Transforming growth factor-β and the initiation of chondrogenesis and osteogenesis in the rat femur. J Cell Biol 110: 2195-2207
14. Lee K, Deeds JD, Chiba S, Un-No M, Bond AT, Segre GV (1994) Parathyroid hormone induces sequential c-fos expression in bone cells in vivo: in situ localization of its receptor and c-fos messenger ribonucleic acids. Endocrinology 134: 441-450
15. Seyedin SM, Thomas TC, Thompson AY, Rosen DM, Piez KA (1985) Purification and characterization of two cartilage-inducing factors from bovine demineralized bone. Proc Natl Acad Sci USA 82:2267-2271
16. Noda M, Camilliere JJ (1989) In vivo stimulation of bone formation by transforming growth factor β. Endocrinology 124:2991-2994
17. Oreffo RO, Mundy GR, Seyedin SM, Bonewald LF (1989) Activation of the latent bone-derived TGF beta complex by isolated osteoclasts. Biochem Biophys Res Commun 158: 817-823
18. Yamazaki K, Eyden BP (1995) A study of intercellular relationships between trabecular bone and marrow stromal cells in the murine femoral metaphysis. Anat Embryol 192:9-20
19. Skerry TM, Bitensky L, Lanyon LE (1989) Early strain-related changes in enzyme activity in osteocytes following bone loading in vivo. J Bone Miner Res 4:783-788
20. Bourret LA, Rodan GA (1976) The role of calcium in the inhibition of cAMP accumulation in epiphyseal cartilage cells exposed to physiological pressure. J Cell Physiol 98:299-306
21. El Haj AJ, Minter SL, Rawlinson SCF, Suswillo R, Lanyon LE (1990) Cellular responses to mechanical loading in vitro. J Bone Miner Res 5:923-932
22. Rawlinson SCF, El Haj AJ, Minter SL, Tavares IA, Bennett A, Lanyon LE (1991) Load-related increases of prostaglandin production in cores of adult canine cancellous bone in vitro - a role for prostacyclin in adaptative bone remodeling? J Bone Miner Res 6:1345-1351
23. Westerlind KC, Turner RT (1995) The skeletal effects of spaceflight in growing rats: tissue-specific alterations in mRNA levels for TGF-β. J Bone Miner Res 10:843-848