Cyanobacterial toxins: The current status

Journal of Microbiology and Biotechnology

Volumn 9, Issue 1, 1999, Pages 9-21

  Tyagi, M B ^a   Thakur, J K ^a   Singh, D P ^a   Kumar, Arvind ^a   Prasuna, E G ^a   Kumar, Ashok ^a  

^a BANARAS HINDU UNIVERSITY   (India)

Author keywords

Anatoxin; Biotoxin; Bloom; Cyanobacteria; Hepatotoxin; Saxitoxin

Indexed keywords

ANATOXIN; BACTERIAL TOXIN; CYANOGINOSIN; NEUROTOXIN; NODULARIN; SAXITOXIN;

BLUE GREEN ALGA; CHEMICAL MODIFICATION; CYANOBACTERIUM; EUTROPHICATION; LIVER TOXICITY; NEUROTOXICITY; NONHUMAN; PHOTOSYNTHESIS; REVIEW; TOXIN ANALYSIS;

ALGAE; BACTERIA (MICROORGANISMS); CHLOROPHYTA; CYANOBACTERIA;

EID: 0033021121     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (107)

1. Arment, A. R. and W. W. Carmichael. 1996. Evidences that microcystin is a thio-template product. J. Phycol. 32: 591-597.
2. Aronstom, R. S. and B. Witcop. 1981. Anatoxin-a interactions with colinergic synaptic molecules. Proc. Nad. Acad. Sci. USA 78: 4639-4643.
3. Banker, R., S. Carmeli, O. Hadas, B. Teltsch, R. Porat, and A. Sukeni. 1997. Identification of cylindrospermopsin in Aphanizomenon ovalisporum (cyanophyceae) isolated from lake kinneret, Israel. J. Phycol. 33: 613-616.
4. Beasley, V. R., R. W. Coppock, J. Simon, R. Ely, W. B. Buck, R. A. Corley, D. M. Carlson, and P. R. Gorham. 1983. Apparent blue-green algae poisoning in swine subsequent to ingestion of a bloom dominated by Anabaena spiroides. J. Am. Vet. Med. Assoc. 182: 413-414.
5. Beasley, V. R., W. O. Cook, A. M. Dahlem, S. B. Hooser, R. A. Lovell, and W. M. Valentine. 1989. Algae intoxicaton in livestock and water fowl. Clin. Tox. Vet. Clin. North Am.: Food Animal Prac. 5: 345-361.
6. Bishop, C. T., E. F. L. J. Anet, and P. R. Gorham. 1959. Isolation and identification of a fast-death factor in Microcystis aeruginosa NRC-1. Can. J. Biochem. Physiol. 37: 453-471.
7. Bonjouklian, R., T. A. Smitka, L. E. Doolin, R. M. Molloy, M. Debono, S. A. Shaffer, R. E. Moore, J. B. Stewart, and G. M. L. Patterson. 1991. Tjipanazoles, new antifungal agents from the blue green alga Tolypothrix tjipanasensis. Tetrahedron 47: 7739-7750.
8. Botes, D. P., H. Kruger, and C. C. Viljoen. 1982a. Isolation and characterization of four toxins from the blue-green alga, Microcystis aeruginosa. Toxicon. 20: 945-954.
9. Botes, D. P., C. C. Viljoen, H. Kruger, P. L. Wessels, and D. H. Williams. 1982b. Structure of toxins of the blue-green alga Microcystis aeruginosa. S. Afr. J. Sci. 78: 378-379.
10. Botes, D. P., P. L. Wessels, H. Kruger M. T. C. Runnegar, S. Santikarn, R. J. Smith, J. C. J. Barna, and D. H. Williams. 1985. Structural studies on cyanoginosins -LR, -YR, -YA, and -YM, peptide toxins from Microcystis aeruginosa. J. Chem. Soc. Perkin. Trans. 1: 2747-2748.
11. Carmeli, S., R. E. Moore, G. M. L. Patterson, T. H. Corbett, and F. A. Valeriote. 1990a. Tantazoles: Unusual cytotoxic alkaloids from the blue-green alga Scytonema mirabile. J. Am. Chem. Soc. 112: 8195-8197.
12. Carmeli, S., R. E. Moore, and G. M. L. Patterson. 1990b. Tolytoxin and new scytophycins from three species of Scytonema. J. Nat. Prod. 53: 1533-1542.
13. Carmeli, S., R. E. Moore, and G. M. L. Patterson. 1991. Mirabazoles, minor tantazole related cytotoxins from the terrestrial blue-green alga Scytonema mirabile. Tetrahedron Lett. 32: 2593-2596.
14. Carmichael, W. W. 1988. Toxins of freshwater algae, pp. 121-147. In A.T. Tu (ed.), Handbook of Natural Toxins. Marcel Dekker, New York.
15. Carmichael, W. W. 1992. Cyanobacterial secondary metabolites-the cyanotoxins. J. Appl. Bacteriol. 72: 460-466.
16. Carmichael, W. W. 1994. The toxins of cyanobacteria. Sci. Am. 270: 64-70.
17. Carmichael, W. W. 1996. Toxic Microcystis and the environment, pp. 1-11. In M. F. Watanabe, K.-L, Harada, W. W. Carmichael, and H. Fujiki. (eds.), Toxic Microcystis. CRC Press, Boca Raton.
18. Carmichael, W. W. and P. E. Bent. 1981. Hemagglutination method for detection of freshwater cyanobacteria (blue green algae) toxins. Appl. Environ. Microbiol. 41: 1383-1388.
19. Carmichael, W. W. and P. R. Gorham. 1978. Anatoxins from clones of Anabaena flos-aquae isolated from lakes of western Canada. Mitt. Int. Verein. Limnol. 21: 285-295.
20. Carmichael, W. W. and P. R. Gorham. 1980. Fresh water cyanophyte toxins: Types and their effects on the use of micro-algae biomass. pp. 437-448. In G. Shelef, and C. J. Soeder (eds.), Algae Biomass, Production and Use. Elsevier/ North-Holland Biomedical Press, Amsterdam.
21. Carmichael, W. W., D. F. Biggs, and P. R. Gorham. 1975. Toxicology and pharmacological action of Anabaena flosaquae toxin. Science 187: 542-544.
22. Carmichael, W. W., D. F. Biggs, and M. A. Peterson. 1979. Pharmacology of anatoxin-a, produced by the freshwater cyanophyte Anabaena flos-aquae NRC-44-1. Toxicon. 17: 229-236.
23. Carmichael, W. W., N. A. Mahmood, and E. G. Hyde. 1990. Natural toxins from cyanobacteria (blue-green algae), pp. 87-106. In S. Hall, and G. Strichartz (eds.), Marine Toxins: Origin, Structure and Molecular Pharmacology. American Chemical Society, Washington, D.C.
24. Carmichael, W. W., M.-J. Yu, J.-R. He, J.-W. He, and J.-L. Yu. 1988. Occurrence of the toxic cyanobacterium (blue-green alga) Microcystis aeruginosa in Central China. Arch. Hydrobiol. 114: 21-30.
25. Chapman, A. D. and C. L. Schelske. 1997. Recent appearance of Cylindrospermopsis (cyanobacteria) in five hyper-eutrophic Florida lakes. J. Phycol. 33: 191-195.
26. Chauhan, V. S., J. B. Marwah, and S. N. Bagchi. 1992. Effect of an antibiotic from Oscillatora sp. on phytoplankters, higher plants and mice. New Phytol. 120: 251-257.
27. Codd, G. A. and G. K. Poon, 1988. Cyanobacterial toxins. pp. 283-296. In L. J. Rogers, and J. R. Gallon (eds.), Biochemistry of the Algae and Cyanobacteria. Oxford Sci. Publ., Clarendon Press, Oxford.
28. Codd, G. A., S. G. Bell, and W. P. Brooks. 1989. Cyanobacterial toxins in water. Water Sci. Technol. 23: 1-13.
29. Cood, G. A., W. P. Brooks, I. M. Priestly, G. K. Poon, and S. G. Bell. 1989. Production, detection and quantification of Cyanobacterial toxins. Tox. Assess 4: 499-511.
30. Demott, W. R., Q. X. Zhang, and W. W. Carmichael. 1991. Effects of toxic cyanobacteria and purified toxins on the survival and feeding of a copepod and three species of Daphnia. Limnol. Oceanogr. 36: 1346-1357.
31. Devlin, J. P., O. E. Edwards, P. R. Gorham, N. R. Hunter, R. K. Pike, and B. Stavric. 1977. Anatoxin-a, a toxic alkaloid from Anabaena flos-aquae NRC-44h. Can. J. Chem. 55: 1367-1371.
32. Eriksson, J. E., J. A. O. Meriluoto, and T. Lindholm. 1986. Can Cyanobacterial peptide toxins accumulate in aquatic food chains? pp. 655-658. In F. Megusar and M. Gantar (eds.), Perspectives in Microbial Ecology. Ljublijana, Yugoslavia.
33. Eriksson, J. E., D. Toivola, J. A. O. Meriluoto, H. Karaki, Y.-G. Han, and D. Hartshorne. 1990a. Hepatocyte deformation induced by Cyanobacterial toxins reflects inhibition of protein phosphatases. Biochem. Biophys. Res. Commun. 173: 1347-1353.
34. 3H-dihydromicrocystin-LR, a cyclic peptide toxin. Biochim. Biophys. Acta 1025: 60-66.
35. Falch, B. S., G. M. Konig, A. D. Wright, O. Sticher, C. K. Angerhofer, J. M. Pezzuto, and H. Bachmann. 1995. Biological activities of cyanobacteria: evaluation of extract and pure compounds. Planta Med. 61: 321-328.
36. Falconer, I. R. 1989. Tumour promotion and liver injuries caused by oral consumption by cyanobacteria. Environ. Toxicol. Water Qual. 6: 177-184.
37. Falconer, I. R., J. V. Smith, A. R. B. Jackson, A. Jones, and M. T. C. Runnegar. 1988. Oral toxicity of a bloom of the cyanobacterium Microcystis aeruginosa administered to mice over periods up to one year. J. Toxicol. Environ. Health. 24:291-305.
38. Francis, G. 1878. Poisonous Australian lake. Nature 18: 11-12.
39. Frankmolle, W. P., L. K. Larsen, F. R. Caplan, G. M. L. Patterson, G. Knubel, I. A. Levine, and R. E. Moore. 1992. Antifungal cyclic peptides from the terrestrial blue-green alga Anabaena laxa. I. Isolation and biological properties. J. Antibiot. 45: 1451-1457.
40. Gleason, F. K. and J. M. Wood. 1987. Secondary metabolism in the cyanobacteria. pp. 437-452. In P. Fay and C. Van. Baalen (eds.), The Cyanobacteria. Elsevier, Amsterdam.
41. Gorham, P. R. and W. W. Carmichael. 1988. Hazards of freshwater blue-greens (cyanobacteria). pp. 403-431. In C. A. Lembi, and J. R. Waaland (eds.), Algae and Human Affairs. Cambridge University Press, Cambridge.
42. Grauer, F. H. and H. L. Arnold Jr. 1961. Seaweed dermatitis: First report of a dermatitis-producing marine alga.Arch. Dermatol. 84: 720-732.
43. Harada, K.-I., M. Suzuki, A. M. Dahlem, V. R. Beasley, W. W. Carmichael, and K. L. Rinehart. 1988. Improved method for purification of toxic peptides produced by cyanobacteria. Toxicon. 26: 433-439.
44. Harada, K.-I., I. Ohtani, K. Iwamoto, M. Suzuki, M. F. Watanabe, M. Watanabe, and K. Terao. 1994. Isolation of cylindrospermopsin from a cyanobacterium Umezakia natans and its screening method. Toxicon. 32: 73-84.
45. Harada, K.-I, K. Ogawa, Y. Kimura, H. Murata, M. Suzuki, P. M. Thorn, W. R. Evans, and W. W. Carmichael. 1991. Microcystins from Anabaena flos-aquae NRC 525-17. Chem. Res. Toxicol 4: 534-540.
46. Hayakawa, K. and K. Kohama, 1995. Reversible effects of okadaic acid and microcystin-LR on the ATP-dependent interaction between actin and myosin. J. Biochem. 117: 509-514.
47. Heise, H. A. 1951. Symptoms of hay fever caused by algae. II. Microcystis: Another form of algae producing allergenic reactions. Ann. Allergy. 9: 100-101.
48. Hooser, S. B., V. R. Beasley, L. L. Waite, M. S. Kuhlenschmidt, W. W. Carmichael, and W. M. Haschek. 1991. Actin filament alterations in rat hepatocytes induced in vivo and in vitro by microcystin-LR, a hepatotoxin from the blue-green alga, Microcystis aerginosa. Vet. Pathol. 28: 259-266.
49. Huber, C. S. 1972. The crystal structure and absolute configuration of 2,9,-diacetyl-9-azabicyclo(4,2,1) non-2,3-ene. Acta Crytallograph. B28: 2577-2582.
50. Humpage, A. R., J. Rositano, A. H. Bretag, R. Brown, P. D. Baker, B. C. Nichoson, and D. A. Steffensen. 1994. Paralytic shellfish poisons from Australian cyanobacterial blooms. Australian J. Mar. Freshwater Res. 45: 761-771.
51. Hyde, E. G. and W. W. Carmichael. 1991. Anatoxin-a(s), a naturally occurring organophosphate, is an irreversible active site-directed inhibitor of acetylcholinesterase (EC 3. 1.1.7) J. Biochem. Toxicol. 6: 195-201.
52. Ikawa, M., K. Wegner, T. L. Foxall, and J. J. Sasner Jr. 1982. Comparison of the toxins of the blue-green alga Aphanizomenon flos-aquae with the Gonyaulax toxins. Toxicon. 20: 747-752.
53. Ishibashi, M., R. E. Moore, G. M. L. Patterson, C. F. Xu, and J. Clardy. 1986. Scytophycins, cytotoxic and antimycotic agents from the cyanophyte Scytonema pseudohofmanni. J. Org. Chem. 51: 5300-5306.
54. Ito, E., F. Kondo, and K.-I. Harada, 1997. Hepatic necrosis in aged mice by oral administration of microcystin-LR. Toxicon. 35: 231.
55. Keijola, A. M., K. Himberg, A. L. Esala, K. Sivonen, and L. Hiisvirta. 1988. Removal of cyanobacterial toxins in water treatment processes: Laboratory and pilot-scale experiments. Tox. Assess 3: 643-656.
56. Keleti, G., J. L. Sykora, E. C. Lippy, and M. A. Shapiro. 1979. Composition and biological properties of lipopolysaccharides isolated from Schizothrix calcicola (Ag.) Gomont (cyanobacteria). Appl. Environ. Microbiol. 38: 471-477.
57. Keleti, G., J. L. Sykora, L. A. Maiolie, D. L. Doerfler, and I. M. Campbell. 1981. Isolation and characterization of endotoxin from cyanobacteria (blue-green algae), pp. 447-464. In W. W. Carmichael (ed), The Water Environment: Algal Toxins and Health. Plenum Press, New York.
58. Kiviranta, J., K. Sivonen, S. I. Niemela, and K. Huovinen. 1991. Detection of toxicity of cyanobacteria by Artemia salina bioassay. Environ. Toxicol. Water Qual. 6: 423-436.
59. Knubel, G., L. K. Larsen, R. E. Moore, I. A. Levine, and G. M. L. Patterson. 1990. Cytotoxic, antiviral indolocarbazoles from a blue-green alga belonging to the Nostocaceae. J. Antibiot. 43: 1236-1239.
60. Krishnamurthy, T., W. W. Carmichael, and E. W. Sarver. 1986. Toxic peptides from fresh water cyanobacteria (blue-green algae). I. Isolation, purification and characterization of peptides from Microcystis aeruginosa and Anabaena flosaquae. Toxicon. 24: 865-873.
61. Lahti, K., J. Ahtiainen, J. Rapala, K. Sivonen, and S. I. Niemela. 1995. Assessment of rapid bioassays for detecting cyanobacterial toxicity. Lett. Appl. Microbiol. 21: 109-114.
62. Lampert, W. 1981. Toxicity of the blue-green Microcystis aeruginosa: Effective defense mechanism against grazing pressure by Daphnia. Verh. Int. Verein Limnol. 21: 1436-1440.
63. Lawton, L. A., B. J. P. A. Cornish, and A. W. R. Macdonald. 1998. Removal of cyanobacterial toxins (microcystins) and cyanobacterial cells for drinking water using domestic water filters. Water Res. 32: 633-638.
64. Lee, E.-S. J. and F. K. Gleason. 1994. A second algicidal natural product from cyanobacterium, Scytonema hofmanni. Plant Sci. 103: 155-160.
65. Lehtimaki, J., P. Moisander, K. Sivonen, and K. Kononen. 1997. Growth, nitrogen fixation and nodularin production by two Baltic sea cyanobacteria. Appl. Environ. Microbiol. 63: 1647-1656.
66. Lippy, E. C. and J. Erb. 1976. Gastrointestinal illness at Sewickley, Pa. J. Am. Water Works Assoc. 68: 606-610.
67. Luukkainen, R., K. Sivonen, M. Namikoshi, M. Fardig, K. L. Rinehart, and S. I. Niemela. 1993. Isolation and identification of eight microcystins from thirteen Oscillatoria agardhii strains and structure of a new microcystin. Appl. Environ. Microbiol. 59: 2204-2209.
68. Mason, C. P., K. R. Edwards, E. R. Carlson, J. Pignatello, F. K. Gleason, and J. M. Wood. 1982. Isolation of chlorine-containing antibiotic from the freshwater cyanobacterium Scytonema hofmanni. Science 215: 400-402.
69. Matsunaga, S., R. E. Moore, W. P. Niemczura, and W. W. Carmichael 1989. Anatoxin-a(s), a potent anticholinesterase from Anabaena flos-aquae. J. Am. Ghent. Soc. 111: 8021-8023.
70. Matsushima, R., S. Yoshizawa, M. F. Watanabe, K.-I. Harada, M. Furusawa, W. W. Carmichael, and H. Fujiki. 1990. In vitro and in vivo effects of protein phosphatase inhibitors, microcystin and nodularin, on mouse skin and fibroblasts. Biochem. Biophys. Res. Commun. 171: 867-874.
71. Mahmood, N. A. and W. W. Carmichael. 1986. Paralytic shellfish-poisons produced by the freshwater cyanobacterium Aphanizomenon flos-aquae-NH-5. Toxicon. 24: 175-186.
72. Mittal, A., M. K. Aggarwal, and D. N. Schivpuri. 1979. Respiratory allergy to algae: Clinical aspects. Ann. Allergy 42: 253-256.
73. Moore, R. E. 1996. Cyclic peptides and depsipeptides from cyanobacteria a: A review. J. Ind. Microbiol. 16: 134-143.
74. Namikoshi, M., M. Yuan, K. Sivonen, W. W. Carmichael, K. L. Rinehart, R. Rouhiainen, F. Sun, S. Brittain, and A. Otsuki. 1998. Seven new microcystins possessing two L-glutamic acid units, isolated from Anabaena sp. strain 186. Chem. Res. Toxicol. 11: 143-148.
75. Nishiwaki-Matsushima, R., T. Ohta, S. Nishiwaki, M. Suganuma, K. Kohyama, T. Ishikawa, W. W. Carmichael, and H. Fujiki. 1992. Liver tumor promotion by the cyanobacterial cyclic peptide toxin microcystin-LR. J. Cancer Res. Clin. Oncol 118: 420-424.
76. Ohtani, I., R. E. Moore, and M. T. C. Runnegarr. 1992. Cylindrospermopsin, a potent hepatotoxin from the blue-green alga Cylindrospermopsis raciborskii. J. Am. Chem. Soc. 114: 7941-7942.
77. Patterson, G. M. L. and C. M. Bolis. 1997. Fungal cell-wall polysaccharides elicit an antifungal secondary metabolite (phytoalexin) in the cyanobacterium Scytonema ocellatum. J. Phycol. 33: 54-60.
78. Patterson, G. M. L., L. K. Larsen, and R. E. Moore. 1994. Bioactive natural products from blue-green algae. J. Appl. Phycol. 6: 151-157.
79. Patterson, G. M. L., C. L. Baldwin, C. M. Bolis, F. R. Caplan, H. Karuso, L. K. Larsen, I. A. Levine, R. E. Moore, C. S. Nelson, G. D. Tuang, E. Furusawa, S. Furusawa, T. R. Norton, and R. R. Raybourne. 1991. Antineoplastic activity of cultured blue-green algae (cyanobacteria). J. Phycol. 27: 530-536.
80. Phillips, M. J., R. J. Roberts, J. A. Stewart, and G. A. Codd. 1985. The toxicity of the cyanobacterium Microcystis aeruginosa to rainbow trout, Salmo gairdneri Richardson. J. Fish Dis. 8: 339-344.
81. Prinsep, M. R., R. E. Moore, I. A. Levine, and G. M. L. Patterson. 1992. Westiellamide, a bistratamide related cyclic peptide from the blue-green algae Westiellopsis prolifica. J. Nat. Prod. 55: 140-142.
82. Rinehart, K. L., M. Namikoshi, and B. W. Choi. 1994. Structure and biosynthesis of toxins from blue-green algae (cyanobacteria). J. Appl. Phycol. 6: 159-176.
83. Rinehart, K. L., K.-I. Harada, M. Namiloshi, C. Chen, C. A. Harvis, M. H. G. Munro, J. W. Blunt, P. E. Mulligan, V. R. Beasley, A. M. Dahlem, and W. W. Carmichael. 1988. Nodularin, microcystin and the configuration of Adda. J. Am. Chem. Soc. 110: 8557-8558.
84. Repavich, W. M., W. C. Sonzogni, J. H. Standridge, R. E. Wedepohl, and L. F. Meisner. 1990. Cyanobacteria (blue-green algae) in Wisconsin waters: acute and chronic toxicity. Water Res. 24: 225-231.
85. Runnegar, M. T. C. and I. R. Falconer 1986. The in vivo and in vitro biological effects of the peptide hepatotoxin from the blue-green alga Microcystis aeruginosa. S. Afr. J. Sci. 78: 363-366.
86. Runnegar, M. T. C., R. G. Gerdes, and I. R. Falconer. 1991. The uptake of the cyanobacterial hepatotoxin microcystin by isolated rat hepatocytes. Toxicon. 29: 43-51.
87. Santikarn, S., D. H. Williams, R. J. Smith, S. J. Hammond, D. P. Botes, A. Tuinman, P. L. Wessels, C. C. Viljoen, and H. Kruger. 1983. A partial structure for the toxin BE-4 from the blue-green alga, Microcystis aeruginosa. J. Chem. Soc. Chem. Commun. 12: 652-654.
88. Sawyer, P. J., J. H. Gentile, and J. J. Sasner Jr. 1968. Demonstration of a toxin from Aphanizomenon flosaquae(L) Ralfs. Can. J. Microbiol. 14: 1199-1204.
89. Scott, W. E. 1991. Occurrence and significance of toxic cyanobacteria in Southern Africa. Water Sci. Technol. 23: 175-180.
90. Shimizu, Y. 1996. Microalgal metabolites: A new perspective. Annu. Rev. Microbiol. 50: 431-465.
91. Shimizu, Y., M. Norte, A. Hori, A. Genenah, and M. Kobayushi. 1984. Biosynthesis of saxitoxin analogues: the unexpected pathway. J. Am. Chem. Soc. 106: 6433-6434.
92. Sivonen, K. 1990. Effects of light, temperature, nitrate, orthophosphate, and bacteria on growth of and hepatotoxin production by Oscillatoria agardhii strains. Appl. Environ. Microbiol. 56: 2658-2666.
93. Sivonen, K. 1998. Toxins produced by cyanobacteria. pp. 547-568. In M. Miraglia, H. Van Edmond, C. Brera, and J. Gilbert, (eds.), Mycotoxins and Phycotoxins: Development in Chemistry, Toxicology and Food Safety. U.S.A.
94. Sivonen, K., W. W. Carmichael, M. Namikoshi, K. L. Rinehart, A. M. Dahlem, and S. I. Niemela. 1990. Isolation and characterization of hepatotoxic microcystin homologs from the filamentous freshwater cyanobacterium Nostoc sp. strain 152. Appl. Environ. Microbiol. 56: 2650-2657.
95. Sivonen, K., K. Himberg, R. Luukkainen, S. I. Niemela, G. K. Poon, and G. A. Codd. 1989a. Preliminary characterization of neurotoxic cyanobacterial blooms and strains from Finland. Tax. Assess 4: 339-352.
96. Sivonen, K., K. Kononen, W. W. Carmichael, A. M. Dahlem, K. L. Rinehart, J. Kiviranta, and S. I. Niemela. 1989b. Occurrence of the hepatotoxic cyanobacterium Nodularia spumigena in the Baltic sea and the structure of the toxin. Appl. Environ. Microbiol. 55: 1990-1995.
97. Sivonen, K. K., M. Namikoshi, W. R. Evans, W. W. Carmichael, F. Sun, L. Rouliainen, R. Luukkainen, and K. L. Rinehart. 1992. Isolation and characterization of a variety of microcystins from seven strains of the cyanobacterial genus Anabaena. Appl. Environ. Microbiol. 58: 2495-2500.
98. Skulberg, O. M., G. A. Codd, and W. W. Carmichael. 1984. Toxic blue green algal blooms in Europe: A growing problem. Ambio. 13: 244-247.
99. Skulberg, O. M., W. W. Carmichael, R. A. Anderson, S. Matsunaga, R. E. Moore, and R. Skulberg. 1992. Investigations of a neurotoxic Oscillatorrian strain (cyanophyceae) and its toxin. Isolation and characterization of homoanatoxin-a. Environ. Toxicol. Chem. 11: 321-329.
100. Sykora, J. L., G. Keleti, R. Roche, D. R. Volk, E. C. Lippy, G. P. Kay, R. A. Burgess, and M. A. Shapiro. 1980. Endotoxins, algae and Limulus amoebocyte lysate test in drinking water. Water Res. 14: 829-839.
101. Thakur, J. K. 1996. Physiological and biochemical studies on toxin production by cyanobacteria. Ph.D. Thesis. Banaras Hindu University, Varanasi, India.
102. Theiss, W. C., W. W. Carmichael, J. Wyman, and R. Bruner. 1988. Blood pressure and hepatocellular effects of the cyclic heptapeptide toxin produced by Microcystis aeruginosa strain PCC-7820. Toxicon. 26: 603-613.
103. Toivola, D. M., J. E. Eriksson, and D. L. Brautigan 1994. Identification of protein phosphatase 2A as the primary target for microcystin-LR in rat liver homogenates. FEBS Lett. 344: 175-180.
104. Utkilen, H. and N. Gjolme. 1995. Iron-stimulated toxin production in Microcystis aeruginosa. Appl. Environ. Microbiol. 61: 797-800.
105. Watanabe, M. F. and S. Oishi. 1985. Effects of environmental factors on toxicity of a cyanobacterium (Microcystis aeruginosa) under culture conditions. Appl. Environ. Microbiol. 49: 1342-1344.
106. Watanabe, M. F., S. Oishi, K.-I. Harada, K. Matsuura, H. Kawai, and M. Suzuki. 1988. Toxins contained in Microcystis species of cyanobacteria (blue-green algae). Toxicon. 26: 1017-1025.
107. Yu, J.-L, M.-H. Chen, K.-N. Liu, and M.-J. Yu. 1987. Study on the toxicity of water bloom in the Donghu Lake Wuhan. I. Detection of the toxic effect of blue-green algae. Acta Hydrobiol. Sinica 11: 212-218.