Flow cytometry isolation and reverse transcriptase-polymerase chain reaction characterization of human round spermatids in infertile patients

Human Reproduction

Volumn 14, Issue 2, 1999, Pages 379-387

  Ziyyat, Ahmed ^a   Lassalle, Bruno ^a   Testart, Jacques ^a,b   Briot, Pascal ^b   Amar, Edouard ^b   Finaz, Catherine ^a   Lefèvre, Annick ^a  

^a INSERM   (France)

^b AMERICAN HOSPITAL OF PARIS   (France)

Author keywords

Flow cytometry; Obstructive azoospermia; Protamine; Round spermatids

Indexed keywords

CD3 ANTIGEN; PROTAMINE;

ADULT; ARTICLE; AZOOSPERMIA; CELL ISOLATION; CELL SELECTION; CELL SEPARATION; CELL SIZE; CELL VIABILITY; CLINICAL ARTICLE; CLINICAL TRIAL; DIPLOIDY; FLOW CYTOMETRY; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; LYMPHOCYTE; MALE; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPERMATID; SPERMATOZOON DENSITY; TESTIS BIOPSY;

EID: 0033015958     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/humrep/14.2.379     Document Type: Article

References (40)

1. Angelopoulos, T., Krey, L., Mc Cullough, A. et al. (1997) A simple and objective approach to identifying human round spermatids. Hum. Reprod., 12, 2208-2216.
2. Antinori, S., Versaci, C., Dani, G. et al. (1997a) Fertilization with human testicular spermatids: four successful pregnancies. Hum. Reprod., 12, 286-291.
3. Antinori, S., Versci, C., Dani, G. et al. (1997b) Successful fertilization and pregnancy after injection of frozen-thawed round spermatids into human oocytes. Hum. Reprod., 12, 554-556.
4. Araki, Y., Motoyama, M., Yoshida, A. et al. (1997) Intracytoplasmic injection with late spermatids: a successful procedure in achieving childbirth for couples in which the male partner suffers from azoospermia due to deficient spermatogenesis. Fertil. Steril., 67, 559-561.
5. Aslam, I., Robins, A., Dowell, K. et al. (1998) Isolation, purification and assessment of viability of spermatogenic cells from testicular biopsies of azoospermic men. Hum. Reprod., 13, 639-645.
6. Chen, E. Y., Cheng, A., Lee, A. et al. (1991) Sequence of human glucose-6-phosphate dehydrogenase cloned in plasmids and a yeast artificial chromosome. Genomics, 10, 792-800.
7. Clermont, Y. and Leblond, C.P. (1955) Spermiogenesis of man, monkey, ram and other mammals as shown by the periodic acid-Schiff technique. Am. J. Anat., 96, 229-254.
8. Crabbe, E., Verheyen, G., Tournaye, H. et al. (1997) The use of enzymatic procedures to recover testicular germ cells. Hum. Reprod., 12, 1682-1687.
9. Dadoune, J. P. (1997) La spermatide, cette méconnue. Andrologie, 7, 22-33.
10. Devroey, P. Liu, J., Nagy, Z. et al. (1995) Pregnancies after testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI) in non obstructive azoospermia. Hum. Reprod., 10, 1457-1460.
11. Domenjoud, L., Nussbaum, G., Adham, I. et al. (1990) Genomic sequences of human protamines whose genes, PRM1 and PRM2, are clustered. Genomics, 8, 127-133.
12. Edwards, R., Tarin, J., Dean, N. et al. (1994) Are spermatid injections into human oocytes now mandatory? Hum. Reprod., 9, 2217-2219.
13. Fishel, S., Green, S., Bishop, M. et al. (1995) Pregnancy after intracytoplasmic injection of spermatid. Lancet. 245, 1641-1642.
14. Fishel, S., Green, S., Hunter, A. et al. (1997) Human fertilization with round and elongated spermatids. Hum. Repmd., 12, 336-340.
15. Kahraman, S., Polat, G., Samli, M. et al. (1998) Multiple pregnancies obtained by testicular spermatid injection in combination with intracytoplasmic sperm injection. Hum. Reprod., 13, 104-110.
16. Kimura, Y. and Yanagimachi, R. (1995) Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring. Development, 121, 2397-2405.
17. Kolb, J.P., Abadie, A., Paul-Eugène, N. et al. (1993) Ligation of CD23 triggers cyclic AMP generation in human B lymphocytes. J. Immunol. 150. 4798-4809.
18. Lassalle, B., Ziyyat, A., Testart, J. et al. (1999) Flow cytometric method to isolate round spermatids from mouse testis. Hum. Reprod., 14, in press.
19. Meistrich, M., Longtin, J., Brock, W. et al. (19981) Purification of rat spermatogenic cells and preliminary biochemical analysis of these cells. Biol. Reprod., 25, 1065-1077.
20. Mendoza, C. and Tesarik, Y. (1996) The occurrence and identification of round spermatids in the ejaculate of men with nonobstructive azoospermia. Fertil. Steril., 66, 826-829.
21. Ogura, A., Matsuda, J. and Yanagimachi, R. (1994) Birth of normal young after electrofusion of mouse oocytes with round spermatids. Proc. Natl. Acad. Sci. USA, 91, 7460-7462.
22. Silber, S. and Johnson, L. (1998) Are spermatid injections of any clinical value? ROSNI and ROSI revisited. Hum. Reprod., 13, 509-515.
23. Silber, S., Van Steirteghem, A.C., Nagy, Z. et al. (1996) Normal pregnancies resulting from testicular sperm extraction and intracytoplasmic sperm injection for azoospermia due to maturation arrest. Fertil. Steril., 66, 110-117.
24. Sofikitis, N., Miyagawa, I. Agapitos. E. et al. (1994) Reproductive capacity of the nucleus of the male gamete after completion of meiosis. J. Assist. Reprod. Genet., 11, 325-341.
25. Sofikitis, N., Toda, T., Miyagawa, I. et al. (1996) Beneficial effects of electrical stimulation before round spermatid nuclei injections into rabbit oocytes on fertilization and subsequent embryonic development. Fertil. Steril., 65, 176-185.
26. Sofikitis, N., Yamamoto, Y., Miyagawa, I. et al. (1998) Ooplasmic injection of elongating spermatid for the treatment of non-obstuctive azoospermia. Hum. Reprod., 13, 709-714.
27. Takahashi, K., Shichijo, S., Noguchi, M. et al. (1995) Identification of MAGE-1 and MAGE-4 proteins in spermatogonia and primary spermatocytes of testis. Cancer Res., 16, 3478-3482.
28. Talbot, P. and Chacon, R.S. (1981) A triple-stain technique for evaluating normal accrosome reaction of human sperm. J. Exp. Zool., 215, 201-208.
29. Tesarik, Y. and Mendoza, C. (1996) Spermatid injection into oocytes. I. Laboratory techniques and special features of zygote development. Hum. Reprod., 11, 772-779.
30. Tesarik, J., Mendoza, C. and Testard, J. (1995) Viable embryos from injection of round spermatids into oocytes. N. Engt. J. Med., 333, 525.
31. Tesarik, J., Rolet, F., Brami, C. et al. (1996) Spermatid injection into human oocytes. II. Clinical application in the treatment of infertility due to non-obstuctive azoospermia. Hum. Reprod., 11, 780-783.
32. Tesarik, Y., Greco, E. and Mendoza, C. (1998) ROSI, instructions for use: 1997 update. Hum. Reprod., 13, 519-523.
33. Van den Elson, P., Georgopoulos, K., Shepley, B.A. et al. (1986) Exon/intron organization of the genes coding for the delta chains of the human and murine T-cell receptor/T3 complex. Proc. Natl. Acad. Sci. USA, 83, 2944-2948.
34. Van der Bruggen, P., Traversari, C., Chomez, P. et al. (1991) A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma. Science, 254, 1643-1647.
35. Vanderzwalmen, P., Lejeune, B., Nijs, M. et al. (1995) Fertilization of an oocyte microinseminated with a spermatid in an in-vitro fertilization programme. Hum. Reprod., 10, 502-503.
36. Vanderzwalmen, P., Zech, H., Birkenfeld, A. et al. (1997) Intracytoplasmic injection of spermatids retrieved from testicular tissue: influence of testicular pathology, type of selected spermatids and oocyte activation. Hum. Reprod., 12, 1203-1213.
37. Vanderzwalmen, P., Nijs, M., Schoysman, R. et al. (1998) The problems of spermatid microinjection in the human: the need for an accurate morphological appoach and selective methods for viable and normal cells. Hum. Reprod., 13, 515-519.
38. Verheyen, G., Crabbé, E., Joris. H. et al. (1998) Simple and reliable identification of the human round spermatid by inverted phase contrast microscopy. Hum. Reprod., 13, 1570-1577.
39. Yamanaka, K., Sofikitis, N., Miyagawa, I. et al. (1997) Ooplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermia. J. Assist. Reprod. Genet., 14, 55-62.
40. Yebra de, L., Ballesca, J.L., Vanrell, J. et al. (1998) Detection of P2 precursors in the sperm cells of infertile patients who have reduced protamine P2 levels. Fertil. Steril., 69, 755-759.