Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization

Applied and Environmental Microbiology

Volumn 65, Issue 7, 1999, Pages 3226-3228

  Bach, H J ^a   Errampalli, D ^b   Leung, K T ^b   Lee, H ^b   Hartmann, A ^a   Trevors, J T ^b   Munch, J C ^a  

^a INSTITUTE OF EPIDEMIOLOGY   (Germany)

^b UNIVERSITY OF GUELPH   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE;

ARTICLE; BACILLUS CEREUS; BACTERIAL GENE; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; SOUTHERN BLOTTING;

BACILLUS CEREUS; BACILLUS MYCOIDES; BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0033015226     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.7.3226-3228.1999     Document Type: Article

References (20)

1. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919-1925.
2. Ash, C., J. A. E. Farrow, M. Dorsch, E. Stackebrandt, and M. D. Collins. 1991. Comparative analysis of Bacillus anthracis, Bacillus cereus, and related species on the basis of reverse transcriptase sequencing of 16S rRNA. Int. J. Syst. Bacteriol. 41:343-346.
3. Bourque, S. N., J. R. Valero, M. C. Lavoie, and R. C. Levesque. 1995. Comparative analysis of the 16S to 23S ribosomal intergenic spacer sequences of Bacillus thuringiensis strains and subspecies and of closely related species. Appl. Environ. Microbiol. 61:1623-1626.
4. Donovan, W. P., Y. Tan, and A. C. Slaney. 1997. Cloning of the nprA gene for neutral protease A of Bacillus thuringiensis and effect of in vivo deletion of nprA on insecticidal crystal protein. Appl. Environ. Microbiol. 63:2311-2317.
5. Ewing, W. H. 1962. Enterobacteriaceae. Biochemical methods for group differentiation. Publication no. 734. U.S. Department of Health, Education, and Welfare, Public Health Service, Washington, D.C.
6. Hayano, K., M. Takeuchi, and E. Ichishima. 1987. Characterization of a metalloproteinase component extracted from soil. Biol. Fertil. Soils 4:179-183.
7. Krieg, N. R., and J. G. Holt (ed.). 1984. Bergey's manual of systematic bacteriology, vol. 1. Williams & Wilkins, Baltimore, Md.
8. Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208-218.
9. Nakamura, L. K., and M. A. Jackson. 1995. Clarification of the taxonomy of Bacillus mycoides. Int. J. Syst. Bacteriol. 45:46-49.
10. Priest, F. G., D. A. Kaji, Y. B. Rosato, and V. P. Canhos. 1994. Characterization of Bacillus thuringiensis and related bacteria by ribosomal RNA gene restriction fragment length polymorphism. Microbiology 140:1015-1022.
11. Rost, A.-K. 1995. Nonradioactive Northern blot hybridization with DIG-labeled DNA probes, p. 93-114. In T. H. Köhler, D. Laßner, A.-K. Rost, B. Thamm, B. Pustoweit, and H. Remke (ed.), Quantitation of mRNA by polymerase chain reaction. Springer-Verlag, Berlin, Germany.
12. Seki, T., C.-K. Chung, H. Mikami, and Y. Oshima. 1978. Deoxyribonucleic acid homology and taxonomy of the genus Bacillus. Int. J. Syst. Bacteriol. 28:182-189.
13. Sidler, W., E. Niederer, F. Suter, and H. Zuber. 1986. The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase. Biol. Chem. Hoppe-Seyler 367:643-657.
14. Sneath, P. H. A., N. S. Mair, M. E. Sharpe, and J. G. Holt (ed.). 1986. Bergey's manual of systematic bacteriology, vol. 2. Williams & Wilkins, Baltimore, Md.
15. Somervilie, H. J., and M. L. Jones. 1972. DNA competition studies within the Bacillus cereus group of bacilli. J. Gen. Microbiol. 73:257-265.
16. Watanabe, K., and K. Hayano. 1993. Distribution and identification of proteolytic Bacillus spp. in paddy field soil under rice cultivation. Can. J. Microbiol. 39:674-680.
17. Watanabe, K., and K. Hayano. 1993. Source of soil protease in paddy field. Can. J. Microbiol. 39:1035-1040.
18. Watanabe, K., and K. Hayano. 1994. Source of soil protease based on the splitting sites of a polypeptide. Soil Sci. Plant Nutr. 40:697-701.
19. Wetmore, D. R., S. L. Wong, and R. S. Roche. 1991. The role of the prosequence in the processing and secretion of the thermolysin-like neutral protease from Bacillus cereus. Mol. Microbiol. 6:1593-1604.
20. Wintzingerode, F., F. A. Rainey, R. M. Kroppenstedt, and E. Stackebrandt. 1997. Identification of environmental strains of Bacillus mycoides by fatty acid analysis and species-specific 16S rDNA oligonucleotide probe. FEMS Microbiol. Ecol. 24:201-209.