Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons

Journal of Bacteriology

Volumn 181, Issue 12, 1999, Pages 3803-3809

  Asai, Tsuneaki ^a,b   Condon, Ciarán ^a,c   Voulgaris, Justina ^a,d   Zaporojets, Dmitry ^a   Shen, Binghua ^a   Al Omar, Michaal ^a   Squires, Craig ^a   Squires, Catherine L ^a  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c INST DE BIOLOGIE PHYSICO CHIMIQUE   (France)

^d Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL RNA; RNA 16S; RNA 23S;

ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL GROWTH; CHROMOSOME; CONTROLLED STUDY; ESCHERICHIA COLI; GENE DELETION; NONHUMAN; OPERON; PLASMID; PRIORITY JOURNAL; RIBOSOME;

ESCHERICHIA COLI;

EID: 0033011939     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.12.3803-3809.1999     Document Type: Article

References (35)

1. Asai, T., D. Zaporojets, C. Squires, and C. L. Squires. 1999. An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria. Proc. Natl. Acad. Sci. USA 96: 1971-1976.
2. Beresford, T., and S. Condon. 1991. Cloning and partial characterization of genes for ribonucleic acid in Lactococcus lactis subsp. lactis. FEMS Microbiol. Lett. 62:319-323.
3. Blomfield, I. C., V. Vaughn, R. F. Rest, and B. I. Eisenstein. 1991. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol. Microbiol. 5:1447-1457.
4. Bott, K., G. C. Stewart, and A. G. Anderson. 1984. Genetic mapping of cloned ribosomal RNA genes, p. 19-34. In J. A. Hoch and A. T. Ganesan (ed.), Syntro Conference on Genetics and Biotechnology of Bacilli. Academic Press, New York, N.Y.
5. Bremer, H., and P. P. Dennis. 1996. Modulation of chemical composition and other parameters of the cell by growth rate, p. 1553-1569. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 2. ASM Press, Washington, D.C.
6. Clarke, L., and J. Carbon. 1976. A colony bank containing synthetic Col E1 hybrid plasmids representative of the entire E. coli genome. Cell 9:91-99.
7. Condon, C., S. French, C., Squires, and C. L. Squires. 1993. Deletion of functional ribosomal RNA operons in Escherichia coli causes increased expression of the remaining intact copies. EMBO J. 12:4305-4315.
8. Condon, C., D. Liveris, C. Squires, I. Schwartz, and C. L. Squires. 1995. rRNA operon multiplicity in Escherichia coli and the physiological implications of rrn inactivation. J. Bacteriol. 177:4152-4156.
9. Condon, C., J. Philips, Z.-Y. Fu, C. Squires, and C. L. Squires. 1992. Comparison of the expression of the seven ribosomal RNA operons in Escherichia coli. EMBO J. 11:4175-4185.
10. Condon, C., C. Squires, and C. L. Squires. 1995. Control of rRNA transcription in Escherichia coli. Microbiol. Rev. 59:623-645.
11. Dong, H., L. Nilsson, and C. G. Kurland. 1996. Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates. J. Mol. Biol. 260:649-663.
12. Ellwood, M., and M. Nomura. 1980. Deletion of a ribosomal ribonucleic acid operon in Escherichia coli. J. Bacteriol. 143:1077-1080.
13. Forchhammer, J., and L. Lindahl. 1971. Growth rate of polypeptide chains as a function of the cell growth rate in a mutant of Escherichia coli 15. J. Mol. Biol. 55:563-568.
14. Gaal, T., M. S. Bartlett, W. Ross, C. L. Turnbough, and R. L. Gourse. 1997. Transcription regulation by initiating NTP concentration: rRNA synthesis in bacteria. Science 278:2092-2097.
15. Garnier, T., B. Canard, and S. T. Cole. 1991. Cloning, mapping, and molecular characterization of the rRNA operons of Clostridium perfringens. J. Bacteriol. 173:5431-5438.
16. Gay, P., D. Le Coq, M. Steinmetz, T. Berkelman, and C. I. Kado. 1985. Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. J. Bacteriol. 164:918-921.
17. Godefroy-Colburn, T., A. D. Wolfe, J. Dondon, M. Grunberg-Manago, P. Dessen, and P. Pantaloni. 1975. Light-scattering studies showing the effect of initiation factors on the reversible dissociation of Escherichia coli ribosomes. J. Mol. Biol. 94:461-478.
18. Gourse, R. L., H. A. de Boer, and M. Nomura. 1986. DNA determinants of rRNA synthesis in E. coli: growth rate dependent regulation, feedback inhibition, upstream activation, and antitermination. Cell 44:197-205.
19. Gourse, R. L., T. Gaal, M. S. Bartlett, J. A. Appleman, and W. Ross. 1996. rRNA transcription and growth rate-dependent regulation of ribosome synthesis in Escherichia coli. Annu. Rev. Microbiol. 50:645-677.
20. Gutterson, N. I., and D. E. Koshland. 1983. Replacement and amplification of bacterial genes with sequences altered in vitro. Proc. Natl. Acad. Sci. USA 80:4894-4898.
21. Jinks-Robertson, S., R. Gourse, and M. Nomura. 1983. Expression of rRNA and tRNA genes in Escherichia coli: evidence for feedback regulation by products of rRNA operons. Cell 33:865-876.
22. Jinks-Robertson, S., and M. Nomura. 1987. Ribosomes and tRNA, p. 1358-1385. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology, vol. 2. American Society for Microbiology, Washington, D.C.
23. Keener, J., and M. Nomura. 1996. Regulation of ribosome synthesis, p. 1417-1431. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Washington, D.C.
24. Kiss, A., B. Sain, and P. Venetianer. 1977. The number of rRNA genes in Escherichia coli. FEBS Lett. 79:77-79.
25. Komine, Y., T. Adachi, H. Inokuchi, and H. Ozeki. 1990. Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12. J. Mol. Biol. 212:579-598.
26. LaFauci, G., R. L. Widom, R. L. Eisner, E. D. Jarvis, and R. Rudner. 1986. Mapping of rRNA genes with integrable plasmids in Bacillus subtilis. J. Bacteriol. 165:204-214.
27. Maaløe, O., and N. O. Kjeldgaard. 1966. Control of macromolecular synthesis. W. A. Benjamin, New York, N.Y.
28. Nomura, M., E. A. Morgan, and S. R. Jaskunas. 1977. Genetics of bacterial ribosomes. Annu. Rev. Genet. 11:297-347.
29. Sarmientos, P., J. E. Sylvester, S. Contente, and M. Cashel. 1983. Differential stringent control of the tandem E. coli ribosomal RNA promoters from the rrnA operon expressed in vivo in multicopy plasmids. Cell 32:1337-1346.
30. Singer, M., T. A. Baker, G. Schnitzler, S. M. Deischel, M. Goel, W. Dove, K. J. Jaacks, A. D. Grossman, J. W. Erickson, and C. A. Gross. 1989. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53:1-24.
31. Stater, S., and R. Maurer. 1993. Simple phagemid-based system for generating allele replacements in Escherichia coli. J. Bacteriol. 175:4260-4262.
32. Sussman, J. K., E. L. Simons, and R. W. Simons. 1996. Escherichia coli translation initiation factor 3 discriminates the initiation codon in vivo. Mol. Microbiol. 21:347-360.
33. Triman, K., E. Becker, C. Dammel, J. Katz, H. Mori, S. Douthwaite, C. Yapijakis, S. Yoast, and H. F. Noller. 1989. Isolation of temperature-sensitive mutants of 16S rRNA in Escherichia coli. J. Mol. Biol. 209:645-653.
34. Tulloch, D. L., L. R. Finch, A. J. Hillier, and B. E. Daridson. 1991. Physical map of the chromosome of Lactococcus lactis subsp. lactis DL11 and localization of six putative rRNA operons. J. Bacteriol. 173:2768-2775.
35. Uhlin, B. E., M. R. Volkert, A. J. Clark, A. Sancar, and W. D. Rupp. 1982. Nucleotide sequence of a recA operator mutation. LexA/operator-repressor binding/inducible repair. Mol. Gen. Genet. 185:251-254.