A method for the determination of glucose synthesis in isolated bovine hepatocytes

Journal of Nutritional Biochemistry

Volumn 10, Issue 4, 1999, Pages 205-209

  Azain, M J ^c   Kasser, T R ^b   Baile, C A ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b MONSANTO COMPANY   (United States)

^c NONE

Author keywords

Gluconeogenesis; Hepatocytes; Metabolism

Indexed keywords

CATION EXCHANGE RESIN; FATTY ACID; GLUCAGON; GLUCOSE OXIDASE; ION EXCHANGE RESIN; LACTIC ACID; PROPIONIC ACID;

ANIMAL CELL; ARTICLE; CATTLE; COLUMN CHROMATOGRAPHY; GLUCONEOGENESIS; IN VITRO STUDY; LIVER CELL; NONHUMAN; TECHNIQUE;

BOVINAE;

EID: 0033010760     PISSN: 09552863     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0955-2863(98)00096-5     Document Type: Article

References (19)

1. Ballard I.F.J., Hanson R.W., Kronfeld D.S. Gluconeogenesis and lipogenesis in tissue from ruminant and nonruminant animals. Fed. Proc. 28:1969;218-231.
2. Young J.W. Gluconeogenesis in cattle Significance and methodology . J. Dairy Sci. 60:1977;1-15.
3. Schmidt S.P., Smith J.A., Young J.W. Rapid determination of (carbon-14) glucose specific radioactivity for in vivo glucose kinetics. J. Dairy Sci. 58:1975;952-956.
4. Dong F.M., Freedland R.A. Effects of alanine on gluconeogenesis in isolated rat hepatocytes. J. Nutr. 110:1980;2341-2349.
5. Mills S.E., Armentano L.E., Russell R.W., Young J.W. Rapid and specific isolation of radioactive glucose from biological samples. J. Dairy Sci. 64:1981;1719-1723.
6. Rognstad R. Effects of alteration in energy supply on gluconeogenesis from L-lactate. Int. J. Biochem. 14:1982;765-770.
7. Azain M.J., Martin R.J. Effect of genetic obesity on the regulation of hepatic fatty acid metabolism. Amer. J. Physiol. 244:1983;R400-R406.
8. Forsell J.H., Jesse B.W., Shull L.R. A technique for isolation of bovine hepatocytes. J. Anim. Sci. 60:1985;1597-1609.
9. Reese J.A., Byard J.L. Isolation and culture of adult hepatocytes from liver biopsies. In vitro. 17:1981;935-940.
10. Brown J.W., Leffert H.L., Sell S., Ho R.J. Rapid radioisotopic analysis of glucose synthesis from lactate in primary hepatocyte cultures. Anal. Biochem. 109:1980;284-290.
11. Parvin R., Pande S.V. Microdetermination of (-) carnitine and carnitine acetyltransferase activity. Anal. Biochem. 79:1977;190-201.
12. Faulkner A., Pollock H.T. Effects of glucagon and α- and β-agonists on glycogenolysis and gluconeogenesis in isolated ovine hepatocytes. Biochem. Biophys. Acta. 1052:1990;229-234.
13. Donkin S.S., Armentano L.E. Preparation of extended in vitro cultures of bovine hepatocytes that are hormonally responsive. J. Anim. Sci. 71:1993;2218-2227.
14. Clark M.G., Filsell O.H., Jarrett I.G. Gluconeogenesis in isolated intact lamb liver cells. Biochem. J. 156:1976;671-680.
15. Aiello R.J., Armentano L.E. Effects of volatile fatty acids on propionate metabolism and gluconeogenesis in caprine hepatocytes. J. Dairy Sci. 70:1987;2504-2510.
16. Faulkner A., Pollack H.T. Propionate metabolism and its regulation by fatty acids in ovine hepatocytes. Comp. Biochem. Physiol. 84B:(4):1986;559-565.
17. Lepine A.J., Watford M., Boyd R.D., Ross D.A., Whitehead D.M. Relationship between hepatic fatty acid oxidation and gluconeogenesis in the fasting neonatal pig. Brit. J. Nutrition. 70:1993;81-91.
18. Duee P.H., Pegorier J.P., Peret J., Girard J. Separate effects of fatty acid oxidation and glucagon on gluconeogenesis in isolated hepatocytes from newborn pigs. Bio. Neonate. 47:1985;77-83.
19. Looney M.C., Baldwin R.L., Calvert C.C. Gluconeogenesis in isolated lamb hepatocytes. J. Anim. Sci. 64:1987;283-294.