Plasmid purification using hot Mg2+treatment and no RNase

BioTechniques

Volumn 26, Issue 2, 1999, Pages 194-198

  Ugarov, Victor I ^a   Samatov, Timur R ^a   Chetverina, Helen V ^a   Chetverin, Alexander B ^a  

^a INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

MAGNESIUM ION;

CENTRIFUGATION; ESCHERICHIA COLI; NONHUMAN; PLASMID; PURIFICATION; REVIEW; RNA ANALYSIS;

ESCHERICHIA COLI;

EID: 0033007914     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99262bm03     Document Type: Review

References (7)

1. Birnboim, H.C. and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
2. Del Sal, G., G. Manfioletti and C. Schneider. 1989. The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing. BioTechniques 7:514-520.
3. Gurevich, V.V., I.D. Pokrovskaya, T.A. Obukhova and S.A. Zozulya. 1991. Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases. Anal. Biochem. 195: 207-213.
4. Ishaq, M., B. Wolf and C. Ritter. 1990. Large-scale isolation of plasmid DNA using cetyltrimethylammonium bromide. BioTechniques 9:19-23.
5. Okayama, H. and P. Berg. 1982. High-efficiency cloning of full-length cDNA. Mol. Cell. Biol. 2:161-170.
6. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
7. 2+ improves the purification of plasmid DNA. Trends Genet. 6:140.