An ESR assay for α-amylase activity toward succinylated starch, amylose and amylopectin

Journal of Biochemical and Biophysical Methods

Volumn 38, Issue 3, 1999, Pages 191-202

  Marcazzan, Michele ^a   Vianello, Fabio ^a   Scarpa, Marina ^b   Rigo, Adelio ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b UNIVERSITY OF TRENTO   (Italy)

Author keywords

amylase activity; amylase assay; ESR; Nitroxide labeled polysaccharides; Spin labeling

Indexed keywords

AMYLASE; AMYLOPECTIN; AMYLOSE; DIMETHYL SULFOXIDE; POLYSACCHARIDE; PYRIDINE; STARCH; SUCCINIC ANHYDRIDE;

ARTICLE; COLORIMETRY; ELECTRON SPIN RESONANCE; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESTERIFICATION; INFRARED SPECTROSCOPY; PRIORITY JOURNAL;

ALPHA-AMYLASE; AMYLOPECTIN; AMYLOSE; CARBOXYLIC ACIDS; SOLANUM TUBEROSUM; SPECTROPHOTOMETRY, INFRARED; SPECTRUM ANALYSIS; SUCCINIC ANHYDRIDES; TIME FACTORS; TRITICUM;

EID: 0033005532     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(98)00044-X     Document Type: Article

References (16)

1. Radley JA. Starch and its derivatives. 4th ed. London: Chapman and Hall Ltd, 1968:354-405.
2. Greenwood CT. Starch and glycogen. In: Pigman W, Horton Herp D, editors. The carbohydrates. Chemistry and biochemistry. 2nd ed. Vol. IIB, New York and London: Academic Press, 1972:471-514.
3. Wurzburg OB. Modified starches: properties and uses, Boca Baton: CRC Press, 1986.
4. Rongved P, Fritzell TH, Strande P, Klaveness J. Polysaccharides as carriers for magnetic resonance imaging contrast agents: synthesis and stability of a new amino acid linker derivative. J Carbohydr Res 1996;287:77-89.
5. Karrasch S, Dolder M, Shabert F, Ramsden J, Engel A. Covalent binding of biological samples to solid supports for scanning probe microscopy in buffer solution. Biophys J 1993;65:2437-46.
6. Vianello F, Signor L, Pizzariello A, Di Paolo ML, Scarpa M, Hock B, Giersh T, Rigo A. Continuous flow immunosensor for atrazine detection. Biosens Bioelectron 1998;13:45-53.
7. Patel MC. Acyl derivatives of starch. J Indian Chem Soc Ind News Ed 1953;16:221-2.
8. Aihara H. Succinyl esters of starch. Nippon Shokuhin Gakkaishi 1965;12:410-5.
9. Fisher E, Stein E. α-Amylases. In: Boyer PD, Lardy H, Myrback K, editors. The enzymes. 2nd ed. Vol 4A, New York and London: Academic Press, 1960:313-343.
10. Nakatani H. Monte Carlo simulation of multiple attack mechanism of α-amylase. Biopolymers 1996;39:665-9.
11. Mazur AK, Nakatani H. Multiple attack mechanism in the porcine pancreatic α-amylase hydrolysis of amylose and amylopectin. Arch Biochem Biophys 1993;306:29-38.
12. Bergmayer HU, Grassl M, Walter HE. α-Amylase. In: Bergmeyer HU, editor. Methods of enzymatic analysis. 3rd ed., Vol. 2A, Wheinheim W. Germany - Deerfield Beach FL: VCH, 1983:151-152.
13. Jackson SK, James PE, Rowlands CC, Mile B. Binding of endotoxin to macrophages; interactions of spin-labelled saccharide residues. Biochim Biophys Acta 1992;1135:165-70.
14. Saito K, Yoshioka H, Ito N, Kazama S, Tanizawa H, Lin YJ, Watanabe H, Ogata T, Kamada H. Spatiotemporal ESR-CT study of the metabolism of the spin labelled polysaccharide in a mouse. Biol Pharm Bull 1997;20:904-9.
15. Berliner LJ. Spin labeling theory and applications, London - New York: Academic Press, 1976.
16. Smith IC. The spin label method. In: Swartz H, Bolton J, Borg D, editors. Biological applications of electron spin resonance. New York: Wiley-Interscience, 1972:490-494.