Detection of β-1,6-glucanase isozymes from Trichoderma strains in sodium dodecyl sulphate-polyacrylamide gel electrophoresis and isoelectrofocusing gels

Journal of Microbiological Methods

Volumn 35, Issue 3, 1999, Pages 245-251

  Soler, Andrés ^a   De La Cruz, Jesús ^a   Llobell, Antonio ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

Author keywords

Glucanase; Antagonism; Electrophoresis; Trichoderma

Indexed keywords

CONGO RED; GLUCAN GLUCOSIDASE; ISOENZYME;

ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PURIFICATION; TRICHODERMA;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSIDE HYDROLASES; ISOELECTRIC FOCUSING; ISOENZYMES; SODIUM DODECYL SULFATE; TRICHODERMA;

FUNGI; TRICHODERMA;

EID: 0033001727     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(99)00021-4     Document Type: Article

References (21)

1. Bartnicki-García S. Cell wall chemistry, morphogenesis and taxonomy. Annu. Rev. Microbiol. 22:1968;87-109.
2. Beguin P. Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas. Anal. Biochem. 131:1983;333-336.
3. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
4. Chet I., Benhamou N., Haran S. Trichoderma and Gliocladium. Harman G.E., Kubicek C.P. Mycoparasitism and Lytic Enzymes. 1998;153-172 Taylor and Francis, London.
5. de la Cruz J., Hidalgo-Gallego A., Lora J.M., Benítez T., Pintor-Toro J.A., Llobell A. Isolation and characterization of three chitinases from Trichoderma harzianum. Eur. J. Biochem. 206:1992;856-867.
6. de la Cruz J., Pintor-Toro J.A., Benítez T., Llobell A. Purification and characterization of an endo-β-1,6-glucanase from Trichoderma harzianum related to its mycoparasitism. J. Bacteriol. 177:1995;1864-1987.
7. de la Cruz J., Pintor-Toro J.A., Benítez T., Llobell A., Romero L.C. A novel endo-β-1,3-glucanase, BGN13.1, involved in the mycoparasitism of Trichoderma harzianum. J. Bacteriol. 177:1995;6937-6945.
8. de la Cruz J., Rey M., Lora J.M., Hidalgo-Gallego A., Domínguez F., Pintor-Toro J.A., Llobell A., Benítez T. Carbon source control on β-glucanase, chitobiase and chitinase from Trichoderma harzianum. Arch. Microbiol. 159:1993;316-322.
9. Geremía R., Goldman G.H., Jacobs D., Ardiles W., Vila S.B., van Montagu M., Herrera-Estrella A. Molecular characterization of the proteinase-encoding gene, prb1, related to mycoparasitism by Trichoderma harzianum. Mol. Microbiol. 8:1993;603-613.
10. Hrmova M., Fincher G.B. Purification and properties of three (1→3)-β-D-glucanase isoenzymes from young leaves of barley (Hordeum vulgare). Biochem. J. 289:1993;453-461.
11. Kapteyn J.C., Montijn R.C., Vink E., de la Cruz J., Llobell A., Douwes J.E., Shimoi H., Lipke P.N., Klis F.M. Retention of Saccharomyces cerevisiae cell wall proteins through a phosphodiester-linked β-1,3-/β-1,6-glucan heteropolymer. Glycobiology. 6:1996;337-345.
12. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature. 227:1970;680-685.
13. Leber T.M., Balkwill F.R. Zymography: a single-step staining method for quantification of proteolytic activity on substrate gels. Anal. Biochem. 249:1997;24-28.
14. Lorito M., Farkas V., Rebuffat S., Bodo B., Kubicek C.P. Cell wall synthesis is a major target of mycoparasitic antagonism by Trichoderma harzianum. J. Bacteriol. 178:1996;6382-6385.
15. Lorito M., Hayes C.K., di Pietro A., Woo S.L., Harman G.E. Purification, characterization, and synergistic activity of a glucan 1,3-β-glucosidase and an N-acetyl-β-glucosaminidase from Trichoderma harzianum. Phytopathology. 84:1994;398-405.
16. Lorito M., Woo S.L., D'Ambrosio M., Harman G.E., Hayes C.K., Kubicek C.P., Scala F. Synergistic interaction between cell wall degrading enzymes and membrane affecting compounds. Mol. Plant-Microbe Interactions. 9:1996;206-213.
17. McGrew B.R., Green M.D. Enhanced removal of detergent and recovery of enzymatic activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of casein in gel wash buffer. Anal. Biochem. 189:1990;68-74.
18. Pan S.Q., Ye X.S., Kuc J. Direct detection of beta-1,3-glucanase isozymes on polyacrylamide electrophoresis and isoelectrofocusing gels. Anal. Biochem. 182:1989;136-140.
19. Papavizas G.C. Trichoderma and Gliocladium: biology, ecology and potential for biocontrol. Annu. Rev. Phytopathol. 23:1985;23-54.
20. Pitson S.M., Seviour R.J., McDougall B.M., Stone B.A., Sadek M. Purification and characterization of an extracellular (1→6)-β-glucanase from the filamentous fungus Acremonium persicinum. Biochem. J. 316:1996;841-846.
21. Trudel J., Asselin A. Detection of chitinase activity after polyacrylamide gel electrophoresis. Anal. Biochem. 178:1989;362-366.